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ubiquitin-conjugating enzyme E2E 1

Antibody: CPTC-UBE2E1-1
Background

Catalog Number:

CPTC-UBE2E1-1

RRID:

AB_2617369

Target Antigen:

ubiquitin-conjugating enzyme E2E 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (648.9 KB)

CPTC-UBE2E1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (648.9 KB)

CPTC-UBE2E1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2E1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-UBE2E1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBE2E1-1 as primary Ab against UBE2E1 (rAg 00055) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-UBE2E1 Western blot

Result: Positive

Western Blot using CPTC-UBE2E1-1 as primary Ab against UBE2E1 (rAg 00055) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-UBE2E1-2
Background

Catalog Number:

CPTC-UBE2E1-2

RRID:

AB_2617370

Target Antigen:

ubiquitin-conjugating enzyme E2E 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (927.8 KB)

CPTC-UBE2E1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (927.8 KB)

CPTC-UBE2E1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2E1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-UBE2E1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBE2E1-2 as primary Ab against UBE2E1-2 (rAg 00055) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-UBE2E1-2 Western blot

Result: Positive

Western Blot using CPTC-UBE2E1-2 as primary Ab against UBE2E1-2 (rAg 00055) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-UBE2E1-3
Background

Catalog Number:

CPTC-UBE2E1-3

RRID:

AB_2617371

Target Antigen:

ubiquitin-conjugating enzyme E2E 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (867.3 KB)

CPTC-UBE2E1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (867.3 KB)

CPTC-UBE2E1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2E1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-UBE2E1-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBE2E1-3 as primary Ab against UBE2E1 (rAg 00055) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-UBE2E1-3 Western blot

Result: Positive

Western Blot using CPTC-UBE2E1-3 as primary Ab against UBE2E1 (rAg 00055) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: ubiquitin-conjugating enzyme E2E 1
Background

NCI Identification Number:

00055

Antigen Name:

ubiquitin-conjugating enzyme E2E 1

CPTC Name:

CPTC-UBE2E1

Aliases:

Ubiquitin-Conjugating Enzyme E2E 1; Ubiquitin-Conjugating Enzyme E2E 1; UbcH6; Ubiquitin Carrier Protein E1; Ubiquitin-Protein Ligase E1; UBCH6; EC 6.3.2.19; Ubiquitin-Conjugating Enzyme E2 E1

Function:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.

Chromosomal Localization:

3p24.2

Expression System:

E.coli

Accession Number:

XP_005265488.1

UniProt Accession Number:

P51965

DNA Source:

Imagenes

Immunogen:

Recombinant Full Length Protein

Vector Name:

pNIC28-Bsa4

Extinction Coefficient:

Buffers:

NaP

Expressed Sequence:

MHHHHHHSSGVDLGTENLYFQSMMSDDDSRASTSSSSSSSSNQQTEKETN
TPKKKESKVSMSKNSKLLSTSAKRIQKELADITLDPPPNCSAGPKGDNIY
EWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQ
GVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNR
AEHDRMARQWTKRYAT

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

24088

Last Updated:

10/23/2013

Links

Characterization Data

Gel

Click to enlarge image PAGE of UBE2E1 (rAg 00055) with molecular weight standards in lane 1
Click image to enlarge

CPTC-UBE2E1 PAGE

PAGE of UBE2E1 (rAg 00055) with molecular weight standards in lane 1

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SOPs

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