Ubiquitin Conjugating Enzyme E2C Peptide 2


Background

Catalog Number:

CPTC-UBE2C-3

Target Antigen:

Ubiquitin Conjugating Enzyme E2C Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/26/2019

Antigen Recognition(s):

Peptide, Recombinant Full-length

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

SOP:

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-UBE2C-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3260) Click image to enlarge

CPTC-UBE2C-3 IMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-UBE2C-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3260)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2C-3 peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBE2C-3 as primary Ab against full-length recombinant Ag 10213 (lane 2). Also included are molecular wt. standards (lane 1) and the UBE2C-3 Ab as positive control (lane 3). Click image to enlarge

CPTC-UBE2C-3 Western blot

Result: Positive

Western Blot using CPTC-UBE2C-3 as primary Ab against full-length recombinant Ag 10213 (lane 2). Also included are molecular wt. standards (lane 1) and the UBE2C-3 Ab as positive control (lane 3).


Background

NCI Identification Number:

00051

Antigen Name:

Ubiquitin Conjugating Enzyme E2C Peptide 2

CPTC Name:

CPTC-UBE2C Peptide 2

Keywords:

UBCH10; Ubiquitin Carrier Protein C; Ubiquitin-Protein Ligase C; EC 6.3.2.19; Cyclin-Selective Ubiquitin Carrier Protein; DJ447F3.2; Mitotic-Specific Ubiquitin-Conjugating Enzyme; Ubiquitin Carrier Protein E2-C; Ubiquitin-Conjugating Enzyme E2 C; UbcH10

Function:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or
E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the
E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene.

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro
catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

Chromosomal Localization:

20pq13.12

NCBI Accession Number:

NP_008950.1

Swiss Prot Accession Number:

O00762

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LSLEFPSGYPYNAPTVK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1870

Last Updated:

10/24/2019

Links

Characterization Data

SOPs

No SOPs available.

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