Background
Catalog Number:
CPTC-UBE2C-1
RRID:
AB_2241131
Target Antigen:
Ubiquitin-Conjugating Enzyme E2C
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
SOP:
Purchase
- DSHB CPTC-UBE2C-1
External Links
- Human Protein Atlas CPTC-UBE2C-1
Characterization Data [Compare Characterization Data]
CyTOF
CPTC-UBE2C-1 Immuno-Mass Cytometry
Result: Positive
Imaging mass cytometry on colon cancer tissue core using CPTC-UBE2C-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, colon, pancreas, lung testis, and endometrium, appendix, and kidney) and cancer tissues (breast and colon).
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (172.4 KB)
CPTC-UBE2C-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-UBE2C-1 IHC NCI60
Result: Positive
Immuno-histochemistry of CPTC-UBE2C-1 for NCI60 Cell Line Array at titer 1:250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Characterization SOP Files
IHC Tissue
CPTC-UBE2C-1 IHC Tissue
Result: Positive
Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-UBE2C-1. Titer: 1:250
Characterization SOP Files
Immunofluorescence - HPA
CPTC-UBE2C-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Human assay: A-431 fixed with PFA, dilution: 1:30
Human assay: U-2 OS fixed with PFA, dilution: 1:30
Human assay: U-251 MG fixed with PFA, dilution: 1:30
Characterization SOP Files
Indirect ELISA
CPTC-UBE2C-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-UBE2C-1 NCI60 Protein Array
Result: Positive
Protein Array in which CPTC-UBE2C-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-UBE2C-1 Western Blot
Result: Positive
Western Blot using CPTC-UBE2C-1 as primary Ab against Ag 10213 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10213
Antigen Name:
Ubiquitin-Conjugating Enzyme E2C
CPTC Name:
CPTC-UBE2C
Aliases:
UBE2C; EC 6.3.2.19; UBCH10; dJ447F3.2; OTTHUMP00000031653; OTTHUMP00000031655
Function:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Chromosomal Localization:
20q13.12
Expression System:
E. Coli
Accession Number:
NM_007019
UniProt Accession Number:
O00762
DNA Source:
HIP:HsCD00001519
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
28483
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMASQNRDPAATSVAAARKGAEPSGGAARGPVGKRLQQELMTLMMSGD
KGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVK
FLTPCYHPNVDTQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNIDSP
LNTHAAELWKNPTAFKKYLQETYSKQVTSQEP
Native Sequence:
ASQNRDPAATSVAAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGIS
AFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTP
CYHPNVDTQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNIDSPLNTH
AAELWKNPTAFKKYLQETYSKQVTSQEP
Calculated Isoelectric Point:
6.8
Molecular Weight:
19925
Last Updated:
08/22/2020
Links
- EnsEMBL - EnsEMBL UBE2C
- Uniprot - Uniprot O00762
Characterization Data
Gel
Click image to enlarge
Ag 10213
PAGE of Ag 10213 (with molecular weight standards in lane 1)
Characterization SOP Files
SOPs
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