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SUV39H1 Histone Lysine Methyltransferase Peptide 1

Antibody: CPTC-SUV39H1-1
Background

Catalog Number:

CPTC-SUV39H1-1

Target Antigen:

SUV39H1 Histone Lysine Methyltransferase Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

12/06/2024

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-SUV39H1-1 and BSA conjugated peptide, CLAGLPG-pS-PKKRVR, were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled to the AR2G biosensors. The CPTC-SUV39H1-1 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data were analyzed globally using a 1:2 (bivalent) fitting model. Click image to enlarge

CPTC-SUV39H1-1 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: High Binding

Affinity and binding kinetics of CPTC-SUV39H1-1 and BSA conjugated peptide, CLAGLPG-pS-PKKRVR, were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled to the AR2G biosensors. The CPTC-SUV39H1-1 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data were analyzed globally using a 1:2 (bivalent) fitting model.


Characterization SOP Files

  Flow Cytometry

CPTC-SUV39H1-1 (Flow Cytometry)

Result: Negative

Flow cytometric analysis of thyroid medullary TT cells, asynchronous (blue) or synchronous (serum starved 24 hours, green) using CPTC-SUV39H1-1 rabbit antibody (solid lines) or concentration matched rabbit isotype control antibody (dashed lines). TT cells were permeabilized, fixed, and then stained with either CPTC-SUV39H1-1 or rabbit isotype control antibody. A BV421 conjugated goat anti-rabbit IgG (H+L) was used as a secondary antibody. All data were analyzed using FlowJo.


Characterization SOP Files

  IHC Tissue

CPTC-SUV39H1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-SUV39H1-1 as primary antibody against BSA-conjugated peptide, CLAGLPG-pS-PKKRVR, coated on the plate and detected using the goat anti-rabbit antibody and TMB. Click image to enlarge

CPTC-SUV39H1-1 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-SUV39H1-1 as primary antibody against BSA-conjugated peptide, CLAGLPG-pS-PKKRVR, coated on the plate and detected using the goat anti-rabbit antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-SUV39H1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-SUV39H1-1 as primary antibody against the over-expressed lysate of SUV39H1. The antibody is able to recognize the target protein in the tested lysate (pink signal). The same membrane was probed with an anti CytC antibody and the housekeeping protein was detected (green signal) Click image to enlarge

CPTC-SUV39H1-1 WB (Over-expressed lysates)

Result: Positive

Western Blot using CPTC-SUV39H1-1 as primary antibody against the over-expressed lysate of SUV39H1. The antibody is able to recognize the target protein in the tested lysate (pink signal). The same membrane was probed with an anti CytC antibody and the housekeeping protein was detected (green signal)


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-SUV39H1-1 WB (Cell lysates)

Result: Negative

Western blot using CPTC-SUV39H1-1 as primary antibody against the cell lysates of TT, H146 and H1184 cells, asynchronous (+) or synchronous - serum starved 24hours (-). The antibody was not able to detect the target protein in any of the tested cell lysates. The same membrane was probed with an anti CytC antibody, and all the cell lysates show the presence of the housekeeping protein.


Characterization SOP Files

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Antigen: SUV39H1 Histone Lysine Methyltransferase Peptide 1
Background

NCI Identification Number:

00538

Antigen Name:

SUV39H1 Histone Lysine Methyltransferase Peptide 1

CPTC Name:

CPTC-SUV39H1 Peptide 1

Aliases:

SUV39H1 Histone Lysine Methyltransferase; KMT1A; Suppressor Of Variegation 3-9 Homolog 1; SUV39H; Histone-Lysine N-Methyltransferase SUV39H1; Position-Effect Variegation 3-9 Homolog; Histone H3-K9 Methyltransferase 1; Lysine N-Methyltransferase 1A; Su(Var)3-9 Homolog 1; H3-K9-HMTase 1; Histone-Lysine N-Methyltransferase, H3 Lysine-9 Specific 1; Suppressor Of Variegation 3-9 (Drosophila) Homolog 1; Suppressor Of Variegation 3-9 Homolog 1 (Drosophila); EC 2.1.1.355; EC 2.1.1.43; MG44

Function:

This gene encodes an evolutionarily-conserved protein containing an N-terminal chromodomain and a C-terminal SET domain. The encoded protein is a histone methyltransferase that trimethylates lysine 9 of histone H3, which results in transcriptional gene silencing. Loss of function of this gene disrupts heterochromatin formation and may cause chromosome instability. Alternative splicing results in multiple transcript variants.SUV39H1 (SUV39H1 Histone Lysine Methyltransferase) is a Protein Coding gene. Diseases associated with SUV39H1 include Hutchinson-Gilford Progeria Syndrome and Hyperoxaluria, Primary, Type I. Among its related pathways are RNA Polymerase I Promoter Opening and PKMTs methylate histone lysines. Gene Ontology (GO) annotations related to this gene include chromatin binding and transcription cis-regulatory region binding. An important paralog of this gene is SUV39H2.Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation. Lysine methyltransferases are enyzmes that catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to the lysine residues on histones, particularly histones H3 and H4. The dysregulation of this methylation is critical in the development of cancer.

Chromosomal Localization:

Xp11.23

Accession Number:

UniProt Accession Number:

O43463

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CLAGLPG-pS-PKKRVR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1540

Last Updated:

11/18/2022

Links

Characterization Data

SOPs

No SOPs available.

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