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SRY (Sex Determining Region Y)-Box 9 Peptide 2

Antibody: CPTC-SOX9-2
Background

Catalog Number:

CPTC-SOX9-2

RRID:

AB_2827868

Target Antigen:

SRY (Sex Determining Region Y)-Box 9 Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

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Characterization Data [Compare Characterization Data]
  IP
Download Immuno-precipitation data obtained incubating CPTC-SOX9-2 and the recombinant domain of SOX9 (aa375-509, expected, MW ~18KDa) (143.1 KB)

CPTC-SOX9-2 IPMS

Result: Positive

Immuno-precipitation data obtained incubating CPTC-SOX9-2 and the recombinant domain of SOX9 (aa375-509, expected, MW ~18KDa)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-SOX9-2 as primary antibody against SOX9 domain comprising amino acids 375-509. Click image to enlarge

CPTC-SOX9-2 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-SOX9-2 as primary antibody against SOX9 domain comprising amino acids 375-509.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-SOX9-2 as primary antibody against recombinant SOX9 protein (lane 2) with expected MW of 56 KDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTTC-SOX9-2 recombinant protein WB

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-SOX9-2 as primary antibody against recombinant SOX9 protein (lane 2) with expected MW of 56 KDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-SOX9-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 56.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SOX9-2 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-SOX9-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 56.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-SOX9-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 56.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SOX9-2 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-SOX9-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 56.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: SRY (Sex Determining Region Y)-Box 9 Peptide 2
Background

NCI Identification Number:

00078

Antigen Name:

SRY (Sex Determining Region Y)-Box 9 Peptide 2

CPTC Name:

CPTC-SOX9 Peptide 2

Aliases:

CMD1; CMPD1; SRA1; Campomelic Dysplasia, Autosomal Sex-Reversal; SRY (Sex-Determining Region Y)-Box 9 Protein; SRY-Related HMG-Box, Gene 9; Transcription Factor SOX-9

Function:

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates
transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

SOX9 (SRY (sex determining region Y)-box 9) is a protein-coding gene. Diseases associated with SOX9 include campomelic dysplasia, and campomelic dysplasia with autosomal sex reversal, and among its related super-pathways are Rho Family GTPases and Wnt signaling pathway. GO annotations related to this gene include protein kinase activity and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is SOX13.

Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

Chromosomal Localization:

17q23

Accession Number:

NP_000337.1

UniProt Accession Number:

P48436

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

cRSQYDYTDHQNSSSYYSHA 

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2200

Last Updated:

09/16/2019

Links

Characterization Data

SOPs

No SOPs available.

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