Background
Catalog Number:
CPTC-SNAI2-1
RRID:
AB_2827864
Target Antigen:
Snail Family Zinc Finger 2 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length
Thematic Panel(s):
Epithelial Mesenchymal Transition (EMT)
Purchase
- DSHB CPTC-SNAI2-1
Publications
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (659.4 KB)
CPTC-SNAI2-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IP
Download Immuno-precipitation data obtained incubating CPTC-SNAI2-1 and the recombinant SNAI2 (aa1-268, expected, MW ~32KDa) (145.5 KB)
CPTC-SNAI2-1 IPMS
Result: Negative
Immuno-precipitation data obtained incubating CPTC-SNAI2-1 and the recombinant SNAI2 (aa1-268, expected, MW ~32KDa)
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (659.4 KB)
CPTC-SNAI2-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Indirect ELISA
CPTC-SNAI2-1 Indirect ELISA
Result: Positive
Indirect ELISA using CPTC-SNAI2-1 as primary antibody against full length SLUG protein.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-SNAI2-1 Western Blot (Recombinant Protein)
Result: Presumed Positive (with additional bands)
Western blot using CPTC-SNAI2-1 as primary antibody against full length SLUG protein (lane 2) with expected MW of 32.7KDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-SNAI2-1 Endogenous Western Blot
Result: Negative
Western Blot using CPTC-SNAI2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.0 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-SNAI2-1 Endogenous Automated Western Blot
Result: Negative
Automated Western Blot using CPTC-SNAI2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.0 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00070
Antigen Name:
Snail Family Zinc Finger 2 Peptide 1
CPTC Name:
CPTC-SNAI2 Peptide 1
Aliases:
SLUG; Neural Crest Transcription Factor Slug; Protein Snail Homolog 2; WS2D; Slug Homolog, Zinc Finger Protein; Snail Homolog 2 (Drosophila); SLUGH1; SNAIL2; Slug (Chicken Homolog), Zinc Finger Protein; Snail Homolog 2; Zinc Finger Protein SNAI2; SLUGH
Function:
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.
SNAI2 (snail family zinc finger 2) is a protein-coding gene. Diseases associated with SNAI2 include waardenburg syndrome type iid and piebald trait, snai2-related and among its related super pathways are MAPK Signaling and Kit Receptor Signaling Pathway. GO annotations related to this gene include RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and sequence-specific DNA binding. An important paralog of this gene is SNAI1.
Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis
Chromosomal Localization:
8q11.21
Accession Number:
NP_003059.1
UniProt Accession Number:
O43623
DNA Source:
The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
cPPSDTSSKDHSGSES
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1760
Last Updated:
09/16/2019
Links
- EnsEMBL - EnsEMBL SNAI2
- Entrez - Entrez SNAI2
- Uniprot - Uniprot O43623
Characterization Data
SOPs
No SOPs available.
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