Snail Family Zinc Finger 1 Peptide 1


Background

Catalog Number:

CPTC-SNAI1-1

Target Antigen:

Snail Family Zinc Finger 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/05/2021

Antigen Recognition(s):

Peptide, Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

SOP:

Publications
Characterization Data [Compare Characterization Data]
  IPMS
Download Immuno-precipitation data obtained incubating CPTC-SNAI1-1 and the recombinant SNAI1 (aa1-264, expected, MW ~32KDa) (141.7 KB)

CPTC-SNAI1-1 IPMS

Result: Negative

Immuno-precipitation data obtained incubating CPTC-SNAI1-1 and the recombinant SNAI1 (aa1-264, expected, MW ~32KDa)


  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-SNAI1-1 as primary antibody against full length SNAIL protein. Click image to enlarge

CPTC-SNAI1-1 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-SNAI1-1 as primary antibody against full length SNAIL protein.


Characterization SOP Files

  Western Blot - Cell Lysate
Click to enlarge image Western Blot using CPTC-SNAI1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 29.1 KDa. All cell lysates negative.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SNAI1-1 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-SNAI1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 29.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-SNAI1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 29.1 KDa. All cell lysates negative.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SNAI1-1 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-SNAI1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 29.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-SNAI1-1 as primary antibody against full length SNAIL protein (lane 2) with expected MW of 31.8 KDa.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SNAI1-1 Western Blot (Recombinant Protein)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-SNAI1-1 as primary antibody against full length SNAIL protein (lane 2) with expected MW of 31.8 KDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

Background

NCI Identification Number:

00069

Antigen Name:

Snail Family Zinc Finger 1 Peptide 1

CPTC Name:

CPTC-SNAI1 Peptide 1

Keywords:

Protein Sna; Protein Snail Homolog 1; SNAH; Snail 1 (Drosophila Homolog), Zinc Finger Protein; Snail Homolog 1; SLUGH2; SNA; SNAIL; SNAIL1; dJ710H13.1; Snail 1 Homolog; Snail 1 Zinc Finger Protein; Zinc Finger Protein SNAI1

Function:

Chromosomal Localization:

20q13.2

NCBI Accession Number:

NP_005976.2

UniProt Accession Number:

O95863

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LQDSNPEFTFQQPYDQAHc

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2090

Last Updated:

08/23/2020

Links

Characterization Data

SOPs

No SOPs available.

Don't have Adobe Reader™?

Get it for free at Adobe.com