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Sialic Acid Binding Ig Like Lectin 7 Peptide 1

Antibody: CPTC-SIGLEC7-1
Background

Catalog Number:

CPTC-SIGLEC7-1

Target Antigen:

Sialic Acid Binding Ig Like Lectin 7 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide, Endogenous

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External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (501.3 KB)

CPTC-SIGLEC7-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (501.3 KB)

CPTC-SIGLEC7-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-SIGLEC7-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate

CPTC-SIGLEC7-1 Western Blot Over-Expressed Lysate

Result: Negative

Western blot using CPTC-SIGLEC7-1 as primary antibody against SIGLEC7 HEK293T cell transient overexpression lysate (lane 2). Expected molecular weight - 41.1 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-SIGLEC7-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 51.1 kDa, 41.2 kDa, 16.8 kDa, and 16.7 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SIGLEC7-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-SIGLEC7-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 51.1 kDa, 41.2 kDa, 16.8 kDa, and 16.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-SIGLEC7-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 51.1 kDa, 41.2 kDa, 16.8 kDa, and 16.7 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. All other cell lines are negative. Click image to enlarge

CPTC-SIGLEC7-1 Western Blot Cell Lysate

Result: Positive

Western blot using CPTC-SIGLEC7-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 51.1 kDa, 41.2 kDa, 16.8 kDa, and 16.7 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. All other cell lines are negative.


Characterization SOP Files

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Antigen: Sialic Acid Binding Ig Like Lectin 7 Peptide 1
Background

NCI Identification Number:

00436

Antigen Name:

Sialic Acid Binding Ig Like Lectin 7 Peptide 1

CPTC Name:

CPTC-SIGLEC7 Peptide 1

Aliases:

Sialic Acid Binding Ig Like Lectin 7; Sialic Acid-Binding Ig-Like Lectin 7; QA79 Membrane Protein; P75/AIRM1; D-Siglec; SIGLEC-7; CDw328; AIRM-1; AIRM1; CD328; QA79; P75; Sialic Acid Binding Ig-Like Lectin 19, Pseudogene; Adhesion Inhibitory Receptor Molecule 1, Siglec-7; Sialic Acid Binding Ig-Like Lectin, Pseudogene 2; Sialic Acid Binding Immunoglobulin-Like Lectin 7; Adhesion Inhibitory Receptor Molecule 1; Sialic Acid Binding Ig-Like Lectin 7; CD328 Antigen; SIGLEC19P; SIGLECP2; Siglec-7; SIGLEC7

Function:

SIGLEC7 (Sialic Acid Binding Ig Like Lectin 7) is a Protein Coding gene. Diseases associated with SIGLEC7 include Congenital Disorder Of Glycosylation, Type Iic. Among its related pathways are Hematopoietic Stem Cells and Lineage-specific Markers and Innate Immune System. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC12.Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).

Chromosomal Localization:

19q13.41

Accession Number:

NP_055200.1

UniProt Accession Number:

Q9Y286

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

FHLLGDPQTK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

09/29/2020

Links

Characterization Data

SOPs

No SOPs available.

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