Catalog Number:
CPTC-SCAMP3-1
Target Antigen:
Secretory Carrier Membrane Protein 3 Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Phosphorylation
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
iMRM screenign of CPTC-SCAMP#-1 against synthetic peptide NYGS-pY-STQASAAAATAELLK. THe antibody is not able to pull down the peptide NYGSYSTQASAAAATAELLK.
Result: Positive
iMRM data obtaiend form the incubation of the cell lysate H3255 digest with clone CPTC-SCAMP3-1. Data were acquired on the nano-chip-LC using a 1260 Infinity Series HPLC-Chip cube interface (Agilent, Palo Alto, CA) coupled to a 6495-triple quadrupole mass spectrometer (Agilent). A large capacity chip system (G4240-62010) consisting of a 160 nl enrichment column and a 150 mm*75 um analytical column (Zorbax 300SB-C18, 5 um, 30 A pore size) was used. The clone is able to capture the endogenous target peptide NYGSpYSTQASAAAATAELLK (CPTC-SCAMP3 Peptide1).
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Presumed Positive (with additional bands)
Western Blot using CPTC-SCAMP#-1 against SCAMP3 recombinant protein.
Result: Negative
Western Blot using CPTC-SCAMP3-1 as primary antibody against cell lysates OVCAR3, HCT116, PC-3, MBA-MD-231 and A549 (lane 2) with expected MW of 38 KDa. Inconclusive/negative for all of the screened cell lysates. Molecular weight standards are also included (lane 1). ECL detection.
NCI Identification Number:
00342
Antigen Name:
Secretory Carrier Membrane Protein 3 Peptide 1
CPTC Name:
CPTC-SCAMP3 Peptide 1
Aliases:
Secretory Carrier Membrane Protein 3; Secretory Carrier-Associated Membrane Protein 3; Propin 1; C1orf3; PROPIN1; SCAMP3
Function:
This gene encodes an integral membrane protein that belongs to the secretory carrier membrane protein family. The encoded protein functions as a carrier to the cell surface in post-golgi recycling pathways. This protein is also involved in protein trafficking in endosomal pathways. Two transcript variants encoding different isoforms have been found for this gene.SCAMP3 (Secretory Carrier Membrane Protein 3) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include ubiquitin protein ligase binding. An important paralog of this gene is SCAMP1.Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.
Chromosomal Localization:
1q22
Accession Number:
NP_005689.2
UniProt Accession Number:
O14828
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
NYGS-pY-STQASAAAATAELLK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2200
Last Updated:
08/25/2020
No SOPs available.
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