PRINT

Secretory Carrier Membrane Protein 3 Peptide 1

Antibody: CPTC-SCAMP3-1
Background

Catalog Number:

CPTC-SCAMP3-1

Target Antigen:

Secretory Carrier Membrane Protein 3 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Phosphorylation

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (479.6 KB)

CPTC-SCAMP3-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screenign of CPTC-SCAMP#-1 against synthetic peptide NYGS-pY-STQASAAAATAELLK. THe antibody is not able to pull down the peptide NYGSYSTQASAAAATAELLK. Click image to enlarge

CPTC-SCAMP3-1 iMRM

Result: Positive

iMRM screenign of CPTC-SCAMP#-1 against synthetic peptide NYGS-pY-STQASAAAATAELLK. THe antibody is not able to pull down the peptide NYGSYSTQASAAAATAELLK.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM data obtaiend form the incubation of the cell lysate H3255 digest with clone CPTC-SCAMP3-1. Data were acquired on the nano-chip-LC using a 1260 Infinity Series HPLC-Chip cube interface (Agilent, Palo Alto, CA) coupled to a 6495-triple quadrupole mass spectrometer (Agilent). A large capacity chip system (G4240-62010) consisting of a 160 nl enrichment column and a 150 mm*75 um analytical column (Zorbax 300SB-C18, 5 um, 30 A pore size) was used. The clone is able to capture the endogenous target peptide NYGSpYSTQASAAAATAELLK (CPTC-SCAMP3 Peptide1). Click image to enlarge

CPTC-SCAMP3-1 iMRM (endogenous peptide)

Result: Positive

iMRM data obtaiend form the incubation of the cell lysate H3255 digest with clone CPTC-SCAMP3-1. Data were acquired on the nano-chip-LC using a 1260 Infinity Series HPLC-Chip cube interface (Agilent, Palo Alto, CA) coupled to a 6495-triple quadrupole mass spectrometer (Agilent). A large capacity chip system (G4240-62010) consisting of a 160 nl enrichment column and a 150 mm*75 um analytical column (Zorbax 300SB-C18, 5 um, 30 A pore size) was used. The clone is able to capture the endogenous target peptide NYGSpYSTQASAAAATAELLK (CPTC-SCAMP3 Peptide1).


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (479.6 KB)

CPTC-SCAMP3-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-SCAMP3-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-SCAMP#-1 against SCAMP3 recombinant protein. Click image to enlarge

CPTC-SCAMP3-1 Recombinant Western Blot

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-SCAMP#-1 against SCAMP3 recombinant protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-SCAMP3-1 as primary antibody against cell lysates OVCAR3, HCT116, PC-3, MBA-MD-231 and A549 (lane 2) with expected MW of 38 KDa. Inconclusive/negative for all of the screened cell lysates. Molecular weight standards are also included (lane 1). ECL detection. Click image to enlarge

CPTC-SCAMP3-1 Cell Lysate Western Blot

Result: Negative

Western Blot using CPTC-SCAMP3-1 as primary antibody against cell lysates OVCAR3, HCT116, PC-3, MBA-MD-231 and A549 (lane 2) with expected MW of 38 KDa. Inconclusive/negative for all of the screened cell lysates. Molecular weight standards are also included (lane 1). ECL detection.


Characterization SOP Files

[top]

Antigen: Secretory Carrier Membrane Protein 3 Peptide 1
Background

NCI Identification Number:

00342

Antigen Name:

Secretory Carrier Membrane Protein 3 Peptide 1

CPTC Name:

CPTC-SCAMP3 Peptide 1

Aliases:

Secretory Carrier Membrane Protein 3; Secretory Carrier-Associated Membrane Protein 3; Propin 1; C1orf3; PROPIN1; SCAMP3

Function:

This gene encodes an integral membrane protein that belongs to the secretory carrier membrane protein family. The encoded protein functions as a carrier to the cell surface in post-golgi recycling pathways. This protein is also involved in protein trafficking in endosomal pathways. Two transcript variants encoding different isoforms have been found for this gene.SCAMP3 (Secretory Carrier Membrane Protein 3) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include ubiquitin protein ligase binding. An important paralog of this gene is SCAMP1.Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.

Chromosomal Localization:

1q22

Accession Number:

NP_005689.2

UniProt Accession Number:

O14828

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NYGS-pY-STQASAAAATAELLK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2200

Last Updated:

08/25/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]