PRINT

RTF1 Peptide 1

Antibody: CPTC-RTF1-1
Background

Catalog Number:

CPTC-RTF1-1

RRID:

AB_2877666

Target Antigen:

RTF1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

09/06/2023

Antigen Recognition(s):

Peptide, Phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (495.6 KB)

CPTC-RTF1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Download Immuno-histochemistry of CPTC-RTF1-1 for NCI60 Cell Line Array with titer 1:1250 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) (66.8 KB)

CPTC-RTF1-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-RTF1-1 for NCI60 Cell Line Array with titer 1:1250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-RTF1-1. Titer: 1:6250 Click image to enlarge

CPTC-RTF1-1 IHC TISSUE

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-RTF1-1. Titer: 1:6250


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-RTF1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line. Click image to enlarge

CPTC-RTF1-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-RTF1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line.


Characterization SOP Files

[top]

Antibody: CPTC-RTF1-2
Background

Catalog Number:

CPTC-RTF1-2

Target Antigen:

RTF1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (241.9 KB)

CPTC-RTF1-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Download Immuno-histochemistry of CPTC-RTF1-2for NCI60 Cell Line Array at titer 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) (98.6 KB)

CPTC-RTF1-2 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-RTF1-2for NCI60 Cell Line Array at titer 0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC NCI60
Download Immuno-histochemistry of CPTC-RTF1-2 for NCI60 Cell Line Array with titer 1:50 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) (98.6 KB)

CPTC-RTF1-2 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-RTF1-2 for NCI60 Cell Line Array with titer 1:50
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-RTF1-1. Titer: 1:6250 Click image to enlarge

CPTC-RTF1-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-RTF1-1. Titer: 1:6250


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-RTF1-2. Titer: 1:50 Click image to enlarge

CPTC-RTF1-2 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-RTF1-2. Titer: 1:50


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-RTF1-2 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line. Click image to enlarge

CPTC-RTF1-2 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-RTF1-2 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line.


Characterization SOP Files

[top]

Antigen: RTF1 Peptide 1
Background

NCI Identification Number:

00286

Antigen Name:

RTF1 Peptide 1

CPTC Name:

CPTC-RTF1 Peptide 1

Aliases:

RTF1 Homolog; Paf1/RNA Polymerase II Complex Component; KIAA0252; RNA Polymerase-Associated Protein RTF1 Homolog RTF1; Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) Rtf1; Ortholog Of Mouse Gene Trap Locus 7; GTL7

Function:

This locus may represent a gene involved in regulation of transcription elongation and chromatin remodeling, based on studies of similar proteins in other organisms. The encoded protein may bind single-stranded DNA.RTF1 (RTF1 Homolog, Paf1/RNA Polymerase II Complex Component) is a Protein Coding gene. Among its related pathways are Protein ubiquitination and Mesodermal Commitment Pathway. Gene Ontology (GO) annotations related to this gene include single-stranded DNA binding.Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex (By similarity).

Chromosomal Localization:

15q15.1

Accession Number:

NP_055953.3

UniProt Accession Number:

Q92541

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CSASDLS(ph)EDLFK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]