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Regulatory Associated Protein Of MTOR Complex 1 Peptide 3

Antibody: CPTC-RPTOR-2
Background

Catalog Number:

CPTC-RPTOR-2

RRID:

AB_2722104

Target Antigen:

Regulatory Associated Protein Of MTOR Complex 1 Peptide 3

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/23/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (290.5 KB)

CPTC-RPTOR-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Microarray core of colon cancer immunohistochemically stained with antibody CPTC-RPTOR-2 Click image to enlarge

CPTC-RPTOR-2 IHC

Result: Positive

Tissue Microarray core of colon cancer immunohistochemically stained with antibody CPTC-RPTOR-2


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RPTOR-2 antibody with CPTC-RPTOR peptide 3 (NCI ID#137) as target Click image to enlarge

CPTC-RPTOR-2 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RPTOR-2 antibody with CPTC-RPTOR peptide 3 (NCI ID#137) as target


Characterization SOP Files

  Immunofluorescence

CPTC-RPTOR-2 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-RPTOR-2 antibody. RPTOR protein expression was not detected.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-RPTOR-2 as primary Ab against recombinant RPTOR (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-RPTOR-2 Western Blot

Result: Positive

Western Blot using CPTC-RPTOR-2 as primary Ab against recombinant RPTOR (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-RPTOR-2 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-RPTOR-2 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-RPTOR-2 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Regulatory Associated Protein Of MTOR Complex 1 Peptide 3
Background

NCI Identification Number:

00137

Antigen Name:

Regulatory Associated Protein Of MTOR Complex 1 Peptide 3

CPTC Name:

CPTC-RPTOR Peptide 3

Aliases:

Regulatory Associated Protein Of MTOR Complex 1; Regulatory Associated Protein Of MTOR, Complex 1; Raptor; P150 Target Of Rapamycin (TOR)-Scaffold Protein Containing WD-Repeats; P150 Target Of Rapamycin (TOR)-Scaffold Protein; Regulatory Associated Protein Of MTOR; KIAA1303; MIP1; KOG1

Function:

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.

RPTOR (Regulatory Associated Protein Of MTOR Complex 1) is a Protein Coding gene. Diseases associated with RPTOR include aqueous misdirection and moyamoya disease. Among its related pathways are Gene Expression and Signaling by GPCR. GO annotations related to this gene include binding and protein complex binding.

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at Thr-389, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.

Chromosomal Localization:

17q25.3

Accession Number:

NP_001156506.1

UniProt Accession Number:

Q8N122

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ALETIGANLQK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1210

Last Updated:

07/12/2016

Links

Characterization Data

SOPs

No SOPs available.

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