RB Transcriptional Corepressor 1 Peptide 1


Background

Catalog Number:

CPTC-RB1-1

RRID:

AB_2722102

Target Antigen:

RB Transcriptional Corepressor 1 Peptide 1

Isotype:

IgG2c

Species:

Mouse Monoclonal Antibody

Last Updated:

11/13/2019

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

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Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-RB1-1 peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-RB1-1 phosho-peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Background

Catalog Number:

CPTC-RB1-2

RRID:

AB_2820275

Target Antigen:

RB Transcriptional Corepressor 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

10/23/2021

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RB1-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3288) Click image to enlarge

CPTC-RB1-2 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RB1-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3288)


Background

Catalog Number:

CPTC-RB1-3

RRID:

AB_2820276

Target Antigen:

RB Transcriptional Corepressor 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

10/23/2021

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RB1-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3251) Click image to enlarge

CPTC-RB1-3 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RB1-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3251)


Background

NCI Identification Number:

00099

Antigen Name:

RB Transcriptional Corepressor 1 Peptide 1

CPTC Name:

CPTC-RB1 Peptide 1

Keywords:

OSRC; RB Transcriptional Corepressor 1; PPP1R130; RB; Protein Phosphatase 1, Regulatory Subunit 130; Prepro-Retinoblastoma-Associated Protein; Retinoblastoma-Associated Protein; Retinoblastoma 1; P110-RB1; P105-Rb; Pp110; OSRC; PRb; Exon 17 Tumor GOS561 Substitution Mutation Causes Premature Stop; GOS563 Exon 17 Substitution Mutation Causes Premature Stop; Retinoblastoma Suspectibility Protein; Osteosarcoma; Rb

Function:

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]RB1 (RB Transcriptional Corepressor 1) is a Protein Coding gene. Diseases associated with RB1 include Retinoblastoma and Small Cell Cancer Of The Lung. Among its related pathways are 4-1BB Pathway and Aberrant regulation of mitotic G1/S transition in cancer due to RB1 defects. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and enzyme binding. An important paralog of this gene is RBL2.Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). RB_HUMAN,P06400(Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. RB_HUMAN,P06400

Chromosomal Localization:

13q14.2

Accession Number:

NP_000312.2

UniProt Accession Number:

P06400

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

IPGGNIYI(pS)PLK (pS807)

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1320

Last Updated:

06/09/2022

Links

Characterization Data

SOPs

No SOPs available.

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