Retinoblastoma 1 Peptide 1


Background

Catalog Number:

CPTC-RB1-1

Target Antigen:

Retinoblastoma 1 Peptide 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

05/05/2015

Antigen Recognition(s):

Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-RB1-1 peptide ELISA

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-RB1-1 phosho-peptide ELISA

Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Background

Catalog Number:

CPTC-RB1-2

Target Antigen:

Retinoblastoma 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/26/2018

Antigen Recognition(s):

peptide, phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RB1-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3288) Click image to enlarge

CPTC-RB1-2 iMRM

Immuno-MRM chromatogram of CPTC-RB1-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3288)

Background

Catalog Number:

CPTC-RB1-3

Target Antigen:

Retinoblastoma 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/26/2018

Antigen Recognition(s):

peptide, phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RB1-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3251) Click image to enlarge

CPTC-RB1-3 iMRM

Immuno-MRM chromatogram of CPTC-RB1-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3251)

Background

NCI Identification Number:

00099

Antigen Name:

Retinoblastoma 1 Peptide 1

CPTC Name:

CPTC-RB1 Peptide 1

Keywords:

OSRC; Prepro-Retinoblastoma-Associated Protein; p105-Rb; pRb; pp110; Osteosarcoma; RB; Retinoblastoma Suspectibility Protein; Retinoblastoma-Associated Protein

Function:

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

RB1 (retinoblastoma 1) is a protein-coding gene. Diseases associated with RB1 include trilateral retinoblastoma, and familial retinoblastoma. GO annotations related to this gene include transcription factor binding and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is RBL2.

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression.

Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS
promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Chromosomal Localization:

13q14.2

Expression System:

NCBI Accession Number:

NP_000312.2

Swiss Prot Accession Number:

P06400

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

IPGGNIYI(pS)PLK (pS807)

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1320

Last Updated:

03/08/2018

Links

Characterization Data

SOPs

No SOPs available.

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