Background
Catalog Number:
CPTC-RAF1-3
RRID:
AB_2827856
Target Antigen:
Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 6
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
10/04/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-RAF1-3
Publications
- A dataset describing a suite of novel antibody reagents for the RAS signaling network (PMID: 31467290)
- Integrated Proteogenomic Characterization across Major Histological Types of Pediatric Brain Cancer (PMID: 33242424)
- Targeted mass-spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP kinase, and AKT signaling (PMID: 34671754)
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (540.6 KB)
CPTC-RAF1-3 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IHC Tissue
CPTC-RAF1-3 IHC
Result: Positive
Tissue Microarray core of lung cancer immunohistochemically stained with antibody CPTC-RAF1-3
Characterization SOP Files
Immunofluorescence
CPTC-RAF1-3 Immunofluorescence
Result: Negative
Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-RAF1-3 antibody. RAF1 protein expression was not detected.
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (540.6 KB)
CPTC-RAF1-3 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
NCI 60 Protein Array
CPTC-RAF1-3 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-RAF1-3 Western Blot
Result: Positive
Western Blot using CPTC-RAF1-3 as primary Ab against recombinant RAF1 (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-RAF1-3 Cell Lysate Western Blot
Result: Positive
Western Blot using CPTC-RAF1-3 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Background
Catalog Number:
CPTC-RAF1-7
Target Antigen:
Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 6
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
10/18/2023
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous, Phosphorylation
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (470.6 KB)
CPTC-RAF1-7 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (470.6 KB)
CPTC-RAF1-7 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Western Blot - Over-expressed transient protein in cell lysate
CPTC-RAF1-7 Western Blot Over-Expressed Cell Lysate
Result: Presumed Positive (with additional bands)
Western blot using CPTC-RAF1-7 as primary antibody against transient overexpression lysate of v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) (lane 2). Molecular weight standards are also included (lane 1). Expected molecular weight – 73.1 kDa.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-RAF1-7 Western Blot Cell Lysates
Result: Positive
Western Blot using CPTC-RAF1-7 as primary antibody against cell lysates A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF-7 (lane 5), MOLT-4 (lane 6), and U87 (lane 7). Molecular weight standards are also included (lane 1). U87 cell line is positive. All other cell lines are negative. Expected molecular weights – 73.0 kDa and 75.4 kDa.
Characterization SOP Files
Background
Catalog Number:
CPTC-RAF1-8
Target Antigen:
Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 6
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
09/11/2024
Antigen Recognition(s):
Peptide, Phosphorylation
Purchase
- DSHB CPTC-RAF1-8
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (440.8 KB)
CPTC-RAF1-8 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (440.8 KB)
CPTC-RAF1-8 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-RAF1-8 Western Blot - Cell Lysate
Result: Negative
Western Blot using CPTC-RAF1-8 as primary antibody against the cell lysates of CCRF-CEM, T-47D, MOLT-4, SN12C, HT-29 and A498. The antibody does not recognize the target protein in the tested lysates.
Characterization SOP Files
Background
NCI Identification Number:
00284
Antigen Name:
Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 6
CPTC Name:
CPTC-RAF1 Peptide 6
Aliases:
Raf-1 Proto-Oncogene, Serine/Threonine Kinase; V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1; C-Raf Proto-Oncogene, Serine/Threonine Kinase; Proto-Oncogene C-RAF; EC 2.7.11.1; Raf-1; CRAF; V-Raf-1 Murine Leukemia Viral Oncogene-Like Protein 1; RAF Proto-Oncogene Serine/Threonine-Protein Kinase; Raf Proto-Oncogene Serine/Threonine Protein Kinase; Oncogene RAF1; EC 2.7.11; CMD1NN; C-Raf; NS5; RAF
Function:
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at Ser-75. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.
Chromosomal Localization:
3p25
Accession Number:
NP_002871.1
UniProt Accession Number:
P04049
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
ST(pS)TPNVHMVSTTLPVDSR
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
2090
Last Updated:
01/03/2019
Links
- Uniprot - Uniprot P04049
Characterization Data
SOPs
No SOPs available.
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