PRINT

Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 3

Antibody: CPTC-RAF1-1
Background

Catalog Number:

CPTC-RAF1-1

RRID:

AB_2877663

Target Antigen:

Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 3

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

10/04/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (446.6 KB)

CPTC-RAF1-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Microarray core of breast cancer immunohistochemically stained with antibody CPTC-RAF-1. Click image to enlarge

CPTC-RAF1-1 IHC

Result: Positive

Tissue Microarray core of breast cancer immunohistochemically stained with antibody CPTC-RAF-1.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RAF1-1 antibody with CPTC-RAF1 peptide 3 (NCI ID#184) as target Click image to enlarge

CPTC-RAF1-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RAF1-1 antibody with CPTC-RAF1 peptide 3 (NCI ID#184) as target


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (446.6 KB)

CPTC-RAF1-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-RAF1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-RAF1-1 as primary Ab against recombinant RAF1 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-RAF1-1 Western Blot

Result: Positive

Western Blot using CPTC-RAF1-1 as primary Ab against recombinant RAF1 (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-RAF1-1 Western Blot - Cell Lysate

Result: Negative

Western Blot using CPTC-RAF1-1 as primary antibody against the cell lysates of CCRF-CEM, T-47D, MOLT-4, SN12C, HT-29 and A498. The antibody does not recognize the target protein in the tested lysates.


Characterization SOP Files

[top]

Antibody: CPTC-RAF1-2
Background

Catalog Number:

CPTC-RAF1-2

RRID:

AB_2868551

Target Antigen:

Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 3

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

10/04/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (497.3 KB)

CPTC-RAF1-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Microarray core of breast cancer immunohistochemically stained with antibody CPTC-RAF1-2 Click image to enlarge

CPTC-RAF1-2

Result: Positive

Tissue Microarray core of breast cancer immunohistochemically stained with antibody CPTC-RAF1-2


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RAF1-2 antibody with CPTC-RAF1 peptide 3 (NCI ID#184) as target Click image to enlarge

CPTC-RAF1-2 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RAF1-2 antibody with CPTC-RAF1 peptide 3 (NCI ID#184) as target


Characterization SOP Files

  Immunofluorescence

CPTC-RAF1-2 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-RAF1-2 antibody. RAF1 protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (497.3 KB)

CPTC-RAF1-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-RAF1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-RAF1-2 as primary Ab against recombinant RAF1 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-RAF1-2 Western Blot

Result: Positive

Western Blot using CPTC-RAF1-2 as primary Ab against recombinant RAF1 (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-RAF1-2 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-RAF1-2 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-RAF1-2 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-RAF1-2 - Cell Lysate

Result: Negative

Western Blot using CPTC-RAF1-2 as primary antibody against the cell lysates of CCRF-CEM, T-47D, MOLT-4, SN12C, HT-29 and A498. The antibody does not recognize the target protein in the tested lysates.


Characterization SOP Files

[top]

Antigen: Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 3
Background

NCI Identification Number:

00184

Antigen Name:

Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 3

CPTC Name:

CPTC-RAF1 Peptide 3

Aliases:

Raf-1 Proto-Oncogene, Serine/Threonine Kinase; V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1; C-Raf Proto-Oncogene, Serine/Threonine Kinase; Proto-Oncogene C-RAF; EC 2.7.11.1; Raf-1; CRAF; V-Raf-1 Murine Leukemia Viral Oncogene-Like Protein 1; RAF Proto-Oncogene Serine/Threonine-Protein Kinase; Raf Proto-Oncogene Serine/Threonine Protein Kinase; Oncogene RAF1; EC 2.7.11; CMD1NN; C-Raf; NS5; RAF

Function:

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at Ser-75. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.

Chromosomal Localization:

3p25

Accession Number:

NP_002871.1

UniProt Accession Number:

P04049

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VVDPTPEQFQAFR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1430

Last Updated:

12/22/2016

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]