Background
Catalog Number:
CPTC-RAC1-1
Target Antigen:
Ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
02/25/2013
Antigen Recognition(s):
recombinant full-length
External Links
- Human Protein Atlas Ras-related C3 Botulinum Toxin Substrate 1 -1
Purchase
- DSHB CPTC-RAC1-1
Characterization Data [Compare Characterization Data]
Western Blot
CPTC-RAC1-1 Western Blot
Western Blot Using CPTC-RAC1-1 as primary Ab against Ag 10295(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Indirect ELISA
CPTC-RAC1-1 Indirect ELISA
Indirect ELISA of CPTC-RAC1-1
Characterization SOP Files
NCI 60 Protein Array
CPTC-RAC1-1 NCI 60 Protein Array
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
IHC NCI60
CPTC-RAC1-1 IHC NCI60
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-RAC1-1 IHC Tissue
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Background
Catalog Number:
CPTC-RAC1-2
Target Antigen:
Ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1)
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
03/07/2013
Antigen Recognition(s):
Purchase
- DSHB CPTC-RAC1-2
Characterization Data [Compare Characterization Data]
Western Blot
CPTC-RAC1-2 Western Blot
Western Blot Using CPTC-RAC1-2 as primary Ab against Ag 10295(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Indirect ELISA
CPTC-RAC1-2 Indirect ELISA
Indirect ELISA of CPTC-RAC1-2
Characterization SOP Files
NCI 60 Protein Array
CPTC-RAC1-2 NCI 60 Protein Array
Protein Array in which CPTC-RAC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Characterization SOP Files
IHC NCI60
CPTC-RAC1-2 IHC NCI60
Immuno-histochemistry of CPTC-RAC1-2 for NCI60 Cell Line Array at titer 1:50
0=NEGATIVE
1=WEAK
2=MODERATE
3=STRONG
Characterization SOP Files
Background
NCI Identification Number:
10295
Antigen Name:
Ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1)
CPTC Name:
CPTC-RAC1
Keywords:
RAC1; MGC111543; MIG5; PAK1; TC-25; p21-Rac1
Function:
Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction
Chromosomal Localization:
7p22
Expression System:
E. Coli
NCBI Accession Number:
BC004247
Swiss Prot Accession Number:
P63000
DNA Source:
HIP:HsCD00003506
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
23108
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVD
GKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKW
YPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAK
EIGAVKYLECSAL TQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL
Native Sequence:
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKP
VNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPE
VRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAKEIG
AVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL
Calculated Isoelectric Point:
8.77
Molecular Weight:
21734
Last Updated:
11/14/2012
Links
- Uniprot - Uniprot RAC1
- EnsEMBL - EnsEMBL RAC1
- Entrez - Entrez RAC1
Characterization Data
Gel
Click image to enlarge
Ag 10295
PAGE of Ag 10295 with molecular weight standards
SOPs
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