Background
Catalog Number:
CPTC-RAC1-1
RRID:
AB_1553767
Target Antigen:
Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family Small GTP Binding Protein Rac1)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-RAC1-1
External Links
- Human Protein Atlas CPTC-RAC1-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (182.3 KB)
CPTC-RAC1-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-RAC1-1 IHC NCI60
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-RAC1-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Immunofluorescence - HPA
CPTC-RAC1-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nucleoli & cytosol.
Human assay: A-431 fixed with PFA, dilution: 1:20
Human assay: U-2 OS fixed with PFA, dilution: 1:20
Human assay: U-251 MG fixed with PFA, dilution: 1:20
Characterization SOP Files
Indirect ELISA
CPTC-RAC1-1 Indirect ELISA
Result: High Binding
Indirect ELISA of CPTC-RAC1-1
Characterization SOP Files
NCI 60 Protein Array
CPTC-RAC1-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-RAC1-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-RAC1-1 Western Blot
Result: Positive
Western Blot Using CPTC-RAC1-1 as primary Ab against Ag 10295(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-RAC1-2
RRID:
AB_2176121
Target Antigen:
Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family Small GTP Binding Protein Rac1)
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/24/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-RAC1-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (180.8 KB)
CPTC-RAC1-2 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-RAC1-2 IHC NCI60
Result: Positive
Immuno-histochemistry of CPTC-RAC1-2 for NCI60 Cell Line Array at titer 1:50
0=NEGATIVE
1=WEAK
2=MODERATE
3=STRONG
Characterization SOP Files
Indirect ELISA
CPTC-RAC1-2 Indirect ELISA
Result: High Binding
Indirect ELISA of CPTC-RAC1-2
Characterization SOP Files
NCI 60 Protein Array
CPTC-RAC1-2 NCI 60 Protein Array
Result: Positive
Protein Array in which CPTC-RAC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-RAC1-2 Western Blot
Result: Positive
Western Blot Using CPTC-RAC1-2 as primary Ab against Ag 10295(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10295
Antigen Name:
Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family Small GTP Binding Protein Rac1)
CPTC Name:
CPTC-RAC1
Aliases:
RAC1; MGC111543; MIG5; PAK1; TC-25; p21-Rac1
Function:
Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction
Chromosomal Localization:
7p22
Expression System:
E. Coli
Accession Number:
BC004247
UniProt Accession Number:
P63000
DNA Source:
HIP:HsCD00003506
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
23108
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVD
GKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKW
YPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAK
EIGAVKYLECSAL TQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL
Native Sequence:
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKP
VNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPE
VRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAKEIG
AVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL
Calculated Isoelectric Point:
8.77
Molecular Weight:
21734
Last Updated:
08/22/2020
Links
- EnsEMBL - EnsEMBL RAC1
- Entrez - Entrez RAC1
- Uniprot - Uniprot P63000
Characterization Data
Gel
Click image to enlarge
Ag 10295
PAGE of Ag 10295 with molecular weight standards
Characterization SOP Files
SOPs
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