
Catalog Number:
CPTC-RAC1-1
Target Antigen:
Ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
11/25/2019
Antigen Recognition(s):
Recombinant Full-length
SOP:
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA of CPTC-RAC1-1
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot Using CPTC-RAC1-1 as primary Ab against Ag 10295(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Catalog Number:
CPTC-RAC1-2
Target Antigen:
Ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1)
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
11/25/2019
Antigen Recognition(s):
Recombinant Full-length, Native Protein
SOP:
Result: Positive
Immuno-histochemistry of CPTC-RAC1-2 for NCI60 Cell Line Array at titer 1:50
0=NEGATIVE
1=WEAK
2=MODERATE
3=STRONG
Result: Positive
Indirect ELISA of CPTC-RAC1-2
Result: Positive
Protein Array in which CPTC-RAC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Result: Positive
Western Blot Using CPTC-RAC1-2 as primary Ab against Ag 10295(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
NCI Identification Number:
10295
Antigen Name:
Ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1)
CPTC Name:
CPTC-RAC1
Keywords:
RAC1; MGC111543; MIG5; PAK1; TC-25; p21-Rac1
Function:
Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction
Chromosomal Localization:
7p22
Expression System:
E. Coli
NCBI Accession Number:
BC004247
Swiss Prot Accession Number:
P63000
DNA Source:
HIP:HsCD00003506
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
23108
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVD
GKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKW
YPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAK
EIGAVKYLECSAL TQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL
Native Sequence:
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKP
VNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPE
VRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAKEIG
AVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL
Calculated Isoelectric Point:
8.77
Molecular Weight:
21734
Last Updated:
11/13/2019
PAGE of Ag 10295 with molecular weight standards
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