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Prostaglandin-Endoperoxide Synthase 2 Peptide 1

Antibody: CPTC-PTGS2-1
Background

Catalog Number:

CPTC-PTGS2-1

RRID:

AB_2889060

Target Antigen:

Prostaglandin-Endoperoxide Synthase 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (288.6 KB)

CPTC-PTGS2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PTGS2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5960) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-PTGS2-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PTGS2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5960)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence

CPTC-PTGS2-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-PTGS2-1 Ab shows no localization of PTGS2 protein.


Characterization SOP Files

  NCI 60 Protein Array

CTPC-PTGS2-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-PTGS2-1 as primary antibody against human COX-2 recombinant protein (lane 2). Expected molecular weight - 95.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-PTGS2-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-PTGS2-1 as primary antibody against human COX-2 recombinant protein (lane 2). Expected molecular weight - 95.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-PTGS2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 95 kDa. Molecular weight standards are also included (lane 1). Data is positive for Jurkat and H226 cell lines. Data is negative/inconclusive for remaining cell lines. Click image to enlarge

CPTC-PTGS2-1 Automated Western Blot (Cell Lysate)

Result: Positive

Automated western blot using CPTC-PTGS2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 95 kDa. Molecular weight standards are also included (lane 1). Data is positive for Jurkat and H226 cell lines. Data is negative/inconclusive for remaining cell lines.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PTGS2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 69 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell lines HeLa, Jurkat, A549 (weak) and NCI H226. Inconclusive data for MCF7. Click image to enlarge

CPTC-PTGS2-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-PTGS2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 69 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell lines HeLa, Jurkat, A549 (weak) and NCI H226. Inconclusive data for MCF7.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PTGS2-1 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-PTGS2-1 as a primary antibody against MCF7, NCI H226 and Jurkat cell lysates. PTGS2 protein expression was not detected.


Characterization SOP Files

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Antigen: Prostaglandin-Endoperoxide Synthase 2 Peptide 1
Background

NCI Identification Number:

00389

Antigen Name:

Prostaglandin-Endoperoxide Synthase 2 Peptide 1

CPTC Name:

CPTC-PTGS2 Peptide 1

Aliases:

Prostaglandin-Endoperoxide Synthase 2; Prostaglandin G/H Synthase 2; COX2; Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase And Cyclooxygenase); Prostaglandin H2 Synthase 2; Cyclooxygenase 2; PGH Synthase 2; EC 1.14.99.1; PGHS-2; PHS II; COX-2; Cyclooxygenase 2b; Cyclooxygenase-2; EC 1.14.99; GRIPGHS; PGG/HS; HCox-2; PHS-2; PTGS2

Function:

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.PTGS2 (Prostaglandin-Endoperoxide Synthase 2) is a Protein Coding gene. Diseases associated with PTGS2 include Gastric Ulcer and Bursitis. Among its related pathways are Neuroscience and GABAergic synapse. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and lipid binding. An important paralog of this gene is PTGS1.Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response (PubMed:7947975, PubMed:7592599, PubMed:9261177, PubMed:16373578, PubMed:22942274, PubMed:26859324, PubMed:27226593, PubMed:11939906, PubMed:19540099). The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes (PubMed:7947975, PubMed:7592599, PubMed:9261177, PubMed:16373578, PubMed:22942274, PubMed:26859324, PubMed:27226593). This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed:7947975, PubMed:7592599, PubMed:9261177, PubMed:16373578, PubMed:22942274, PubMed:26859324, PubMed:27226593). Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins (PubMed:11939906, PubMed:19540099). In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids (PubMed:27642067). Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response (PubMed:22942274). Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols (PubMed:11034610, PubMed:11192938, PubMed:9048568, PubMed:9261177). Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation (PubMed:12391014). Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs) (PubMed:12391014). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2 (PubMed:21206090). In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection (PubMed:26236990). In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE) (PubMed:22068350, PubMed:26282205). During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia (By similarity).

Chromosomal Localization:

1q31.1

Accession Number:

NP_000954.1

UniProt Accession Number:

P35354

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ALPPVPDDCPTPLGVK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1760

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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