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Phosphatase And Tensin Homolog Peptide 4

Antibody: CPTC-PTEN-1
Background

Catalog Number:

CPTC-PTEN-1

RRID:

AB_2617320

Target Antigen:

Phosphatase And Tensin Homolog Peptide 4

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (529.3 KB)

CPTC-PTEN-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Microarray core immunohistochemically stained with antibody CPTC-PTEN-1 Click image to enlarge

CPTC-PTEN-1 IHC

Result: Positive

Tissue Microarray core immunohistochemically stained with antibody CPTC-PTEN-1


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PTEN-1 antibody with CPTC-PTEN peptide 4 (NCI ID#110) as target Click image to enlarge

CPTC-PTEN-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PTEN-1 antibody with CPTC-PTEN peptide 4 (NCI ID#110) as target


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (529.3 KB)

CPTC-PTEN-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot of CPTC-PTEN-1 antibody with recombinant PTEN Click image to enlarge

CPTC-PTEN-1 Western blot

Result: Positive

Western blot of CPTC-PTEN-1 antibody with recombinant PTEN


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-PTEN-1 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PTEN-1 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-PTEN-1 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antibody: CPTC-PTEN-5
Background

Catalog Number:

CPTC-PTEN-5

RRID:

AB_2722097

Target Antigen:

Phosphatase And Tensin Homolog Peptide 4

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (535.8 KB)

CPTC-PTEN-5 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Microarray core immunohistochemically stained with antibody CPTC-PTEN-5 Click image to enlarge

CPTC-PTEN-5 IHC

Result: Positive

Tissue Microarray core immunohistochemically stained with antibody CPTC-PTEN-5


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PTEN-5 antibody with CPTC-PTEN peptide 4 (NCI ID#110) as target Click image to enlarge

CPTC-PTEN-5 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PTEN-5 antibody with CPTC-PTEN peptide 4 (NCI ID#110) as target


Characterization SOP Files

  Immunofluorescence

CPTC-PTEN-5 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-PTEN-5 antibody. PTEN protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (535.8 KB)

CPTC-PTEN-5 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PTEN-5 as primary Ab against recombinant PTEN (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PTEN-5 Western Blot

Result: Positive

Western Blot using CPTC-PTEN-5 as primary Ab against recombinant PTEN (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image western Blot using CPTC-PTEN-5 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PTEN-5 Cell Lysate Western Blot

Result: Positive

western Blot using CPTC-PTEN-5 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antibody: CPTC-PTEN-6
Background

Catalog Number:

CPTC-PTEN-6

RRID:

AB_2722098

Target Antigen:

Phosphatase And Tensin Homolog Peptide 4

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (477.2 KB)

CPTC-PTEN-6 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Microarray core immunohistochemically stained with antibody CPTC-PTEN-6 Click image to enlarge

CPTC-PTEN-6 IHC

Result: Positive

Tissue Microarray core immunohistochemically stained with antibody CPTC-PTEN-6


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PTEN-6 antibody with CPTC-PTEN peptide 4 (NCI ID#110) as target Click image to enlarge

CPTC-PTEN-6 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PTEN-6 antibody with CPTC-PTEN peptide 4 (NCI ID#110) as target


Characterization SOP Files

  Immunofluorescence

CPTC-PTEN-6 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC- PTEN-6 antibody. PTEN protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (477.2 KB)

CPTC-PTEN-6 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PTEN-6 as primary Ab against recombinant PTEN (lane 2). Also included are molecular wt. standards (lane Click image to enlarge

CPTC-PTEN-6 Western Blot

Result: Positive

Western Blot using CPTC-PTEN-6 as primary Ab against recombinant PTEN (lane 2). Also included are molecular wt. standards (lane


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-PTEN-6 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PTEN-6 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-PTEN-6 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antibody: CPTC-PTEN-7
Background

Catalog Number:

CPTC-PTEN-7

RRID:

AB_2722099

Target Antigen:

Phosphatase And Tensin Homolog Peptide 4

Isotype:

IgG2c

Species:

Mouse Monoclonal Antibody

Last Updated:

05/28/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on breast cancer tissue core using CPTC-PTEN-7 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (prostate, colon, pancreas, breast, lung, testis, endometrium, and kidney) and cancer tissues (breast, colon, ovarian, and lung). Click image to enlarge

CPTC-PTEN-7 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on breast cancer tissue core using CPTC-PTEN-7 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (prostate, colon, pancreas, breast, lung, testis, endometrium, and kidney) and cancer tissues (breast, colon, ovarian, and lung).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (616.5 KB)

CPTC-PTEN-7 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Microarray core immunohistochemically stained with antibody CPTC-PTEN-7 Click image to enlarge

CPTC-PTEN-7

Result: Positive

Tissue Microarray core immunohistochemically stained with antibody CPTC-PTEN-7


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PTEN-7 antibody with CPTC-PTEN peptide 4 (NCI ID#110) as target Click image to enlarge

CPTC-PTEN-7 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PTEN-7 antibody with CPTC-PTEN peptide 4 (NCI ID#110) as target


Characterization SOP Files

  Immunofluorescence

CPTC-PTEN-7 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-PTEN-7 antibody. PTEN protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (616.5 KB)

CPTC-PTEN-7 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PTEN-7 as primary Ab against recombinant PTEN (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PTEN-7 Western Blot

Result: Positive

Western Blot using CPTC-PTEN-7 as primary Ab against recombinant PTEN (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-PTEN-7 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PTEN-7 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-PTEN-7 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Phosphatase And Tensin Homolog Peptide 4
Background

NCI Identification Number:

00110

Antigen Name:

Phosphatase And Tensin Homolog Peptide 4

CPTC Name:

CPTC-PTEN Peptide 4

Aliases:

Phosphatase And Tensin Homolog; MMAC1; Mutated In Multiple Advanced Cancers 1; GLM2; CWS1; TEP1; Phosphatidylinositol 3,4,5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein; Phosphatase PTEN; Phosphatidylinositol-3,4,5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase PTEN; MMAC1 Phosphatase And Tensin Homolog Deleted On Chromosome 10; Mitochondrial Phosphatase And Tensin Protein Alpha; Phosphatase And Tensin-Like Protein; Mitochondrial PTENalpha; EC 3.1.3.16; EC 3.1.3.48; EC 3.1.3.67; PTEN1; 10q23del; MHAM; DEC; BZS

Function:

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

PTEN (Phosphatase And Tensin Homolog) is a Protein Coding gene. Diseases associated with PTEN include squamous cell carcinoma, head and neck and cowden syndrome 1. Among its related pathways are PI3K-Akt signaling pathway and PI-3K cascade. GO annotations related to this gene include protein kinase binding and magnesium ion binding. An important paralog of this gene is TNS1.



Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement

Isoform alpha: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1

Chromosomal Localization:

10q23.3

Accession Number:

NP_000305.2

UniProt Accession Number:

P60484

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

AQEALDFYGEVR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1320

Last Updated:

10/01/2019

Links

Characterization Data

SOPs

No SOPs available.

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