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Plexin B2 Peptide 1

Antibody: CPTC-PLXNB2-1
Background

Catalog Number:

CPTC-PLXNB2-1

Target Antigen:

Plexin B2 Peptide 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-PLXNB2-1 and BSA-conjugated peptide (CRDIFYKPFHGDIQCGGHAPGSSKS) were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled over AR2G bio- sensors. Mouse antibody CPTC-PLXNB2-1 was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data was analyzed globally using a 1:2 bivalent fitting model. Click image to enlarge

CPTC-PLXNB2-1 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: High Binding

Affinity and binding kinetics of CPTC-PLXNB2-1 and BSA-conjugated peptide (CRDIFYKPFHGDIQCGGHAPGSSKS) were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled over AR2G bio- sensors. Mouse antibody CPTC-PLXNB2-1 was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data was analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-PLXNB2-1 antibody and BSA-conjugated peptide, PLXNB2-4 (CRDIFYKPFHGDIQCGGHAPGSSKS) were measured using surface plasmon resonance. BSA-peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-PLXNB2-1, mouse antibody, was used as analyte and was titrated at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1 nM. Click image to enlarge

CPTC-PLXNB2-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: High Binding

Affinity and binding kinetics of CPTC-PLXNB2-1 antibody and BSA-conjugated peptide, PLXNB2-4 (CRDIFYKPFHGDIQCGGHAPGSSKS) were measured using surface plasmon resonance. BSA-peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-PLXNB2-1, mouse antibody, was used as analyte and was titrated at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1 nM.


Characterization SOP Files

  Flow Cytometry
Click to enlarge image Flow cytometric analysis of human PLXNB2 cell surface expression on human peripheral blood mononuclear cells (PBMC). PBMC cells were stained with purified CPTC-PLXNB2-1 monoclonal antibody (solid green) and then fixed. PBMC cells were stained with concentration-matched mouse IgG2a isotype control antibody (dashed green) and then fixed. A BV421 conjugated goat anti-mouse IgG (minimal x-reactivity) was used as a secondary antibody. The fluorescence histograms were derived from gated events with forward (FSC) and side (SSC) scatter characteristics of viable monocytes. All data were analyzed using FlowJo. Click image to enlarge

CPTC-PLXNB2-1 (Flow Cytometry)

Result: Positive

Flow cytometric analysis of human PLXNB2 cell surface expression on human peripheral blood mononuclear cells (PBMC). PBMC cells were stained with purified CPTC-PLXNB2-1 monoclonal antibody (solid green) and then fixed. PBMC cells were stained with concentration-matched mouse IgG2a isotype control antibody (dashed green) and then fixed. A BV421 conjugated goat anti-mouse IgG (minimal x-reactivity) was used as a secondary antibody. The fluorescence histograms were derived from gated events with forward (FSC) and side (SSC) scatter characteristics of viable monocytes. All data were analyzed using FlowJo.


Characterization SOP Files

  Immunofluorescence

CPTC-PLXNB2-1 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-PLXNB2-1 as primary antibody against PBMC (human mononuclear cells). CPTC-PLXNB2-1 ab shows no PLXNB2 localization in PBMC.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-PLXNB2-1 as primary mouse antibody against BSA-conjugated PLXNB2-3 (TDSREAFEAYTDHATYC, red) and BSA- conjugated PLXNB2-4 (CRDIFYKPFHGDIQCGGHAPGSSKS, blue) coated on the plate and detected using goat anti-mouse antibody and TMB. Click image to enlarge

CPTC-PLXNB2-1 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-PLXNB2-1 as primary mouse antibody against BSA-conjugated PLXNB2-3 (TDSREAFEAYTDHATYC, red) and BSA- conjugated PLXNB2-4 (CRDIFYKPFHGDIQCGGHAPGSSKS, blue) coated on the plate and detected using goat anti-mouse antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PLXNB2-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image CPTC-PLNXNB2-1 was tested in Simple Western against three different proteins: PLXNB1, PLNXB2 and PLNXB3. In the upper panel, the antibody recognized specifically PLXNB2. In the bottom panel, the same His-tagged recombinant protein were tested using an anti-His tag antibody. and all three proteins were detected. Click image to enlarge

CPTC-PLXNB2-1 - Simple Western (Recombinant Protein)

Result: Positive

CPTC-PLNXNB2-1 was tested in Simple Western against three different proteins: PLXNB1, PLNXB2 and PLNXB3. In the upper panel, the antibody recognized specifically PLXNB2. In the bottom panel, the same His-tagged recombinant protein were tested using an anti-His tag antibody. and all three proteins were detected.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image CPTC-PLXNB2- 1 was tested in Western Blot against three different His-tagged recombinant proteins: PLXNB1, PLXNB2 and PLXNB3. The antibody specifically recognizes PLXNB2 (orange color, as results of overlapping pink and green signal). The same blot was probed with an anti-His tag antibody, and all the three protein were detected (green signal). Click image to enlarge

CPTC-PLXNB2-1 Western Blot (Recombinant Protein)

Result: Positive

CPTC-PLXNB2- 1 was tested in Western Blot against three different His-tagged recombinant proteins: PLXNB1, PLXNB2 and PLXNB3. The antibody specifically recognizes PLXNB2 (orange color, as results of overlapping pink and green signal). The same blot was probed with an anti-His tag antibody, and all the three protein were detected (green signal).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image CPTC-PLNXB2-1 was used to probe PBMCs lysate in Simple Western. The target protein was presumably detected in the tested lysate. The same lysate was probe for the housekeeping protein Vinculin, and the housekeeping protein was detected. CPTC-PLNXB2-1 was also used to probe 7 different cell lysates in Simple Western. The tested lysates were: 293T, HCC-2998, NCI-H322M, SF-539, HCT-15, MOLT-4, and CCRF-CEM. The target protein was not detected in any of the tested lysates. The same lysates were probe for the housekeeping protein Vinculin, and all of them expressed it at different level. Click image to enlarge

CPTC-PLXNB2-1 Simple Western (Cell Lysates)

Result: Presumed Positive (with additional bands)

CPTC-PLNXB2-1 was used to probe PBMCs lysate in Simple Western. The target protein was presumably detected in the tested lysate. The same lysate was probe for the housekeeping protein Vinculin, and the housekeeping protein was detected.
CPTC-PLNXB2-1 was also used to probe 7 different cell lysates in Simple Western. The tested lysates were: 293T, HCC-2998, NCI-H322M, SF-539, HCT-15, MOLT-4, and CCRF-CEM. The target protein was not detected in any of the tested lysates. The same lysates were probe for the housekeeping protein Vinculin, and all of them expressed it at different level.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image CPTC-PLNXB2-1 was used to probe 4 different tissue lysates in Simple Western. The tested lysates were: Brain, Kidney, Liver, and Plasma. The target protein was presumably detected in the tested Kidney and Liver lysates. Click image to enlarge

CPTC-PLXNB2-1 Simple Western (Tissue Lysates)

Result: Presumed Positive (with additional bands)

CPTC-PLNXB2-1 was used to probe 4 different tissue lysates in Simple Western. The tested lysates were: Brain, Kidney, Liver, and Plasma. The target protein was presumably detected in the tested Kidney and Liver lysates.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image CPTC-PLXNB2-1 was used to probe four different tissue lysates. The tissues were brain, kidney, liver and plasma. The target protein was detected in brain, (weak signal), and kidney. It was presumably detected in liver, but not in plasma. The same blot was probed with an anti-Vinculin antibody, and the housekeeping protein was detected only in brain, kidney and liver. Click image to enlarge

CPTC-PLXNB2-1 Western Blot (Tissue Lysates)

Result: Positive

CPTC-PLXNB2-1 was used to probe four different tissue lysates. The tissues were brain, kidney, liver and plasma. The target protein was detected in brain, (weak signal), and kidney. It was presumably detected in liver, but not in plasma. The same blot was probed with an anti-Vinculin antibody, and the housekeeping protein was detected only in brain, kidney and liver.


Characterization SOP Files

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Antibody: CPTC-PLXNB2-2
Background

Catalog Number:

CPTC-PLXNB2-2

Target Antigen:

Plexin B2 Peptide 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-PLXNB2-2 and BSA-conjugated PLXNB2-4- (CRDIFYKPFHGDIQCGGHAPGSSKS) peptide were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled over AR2G bio- sensors. The mouse antibody CPTC- PLXNB2-2 was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data was analyzed globally using a 1:2 bivalent fitting model. Click image to enlarge

CPTC-PLXNB2-2 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: High Binding

Affinity and binding kinetics of CPTC-PLXNB2-2 and BSA-conjugated PLXNB2-4- (CRDIFYKPFHGDIQCGGHAPGSSKS) peptide were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled over AR2G bio- sensors. The mouse antibody CPTC- PLXNB2-2 was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data was analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-PLXNB2-2 antibody and BSA-conjugated peptide, PLXNB2-4 (CRDIFYKPFHGDIQCGGHAPGSSKS) were measured using surface plasmon resonance. BSA-peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-PLXNB2-2, mouse antibody, was used as analyte and was titrated at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1 nM. Click image to enlarge

CPTC-PLXNB2-2 Affinity and Kinetics (Surface Plasmon Resonance)

Result: High Binding

Affinity and binding kinetics of CPTC-PLXNB2-2 antibody and BSA-conjugated peptide, PLXNB2-4 (CRDIFYKPFHGDIQCGGHAPGSSKS) were measured using surface plasmon resonance. BSA-peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-PLXNB2-2, mouse antibody, was used as analyte and was titrated at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1 nM.


Characterization SOP Files

  Flow Cytometry
Click to enlarge image Flow cytometric analysis of human PLXNB2 cell surface expression on human peripheral blood mononuclear cells (PBMC). PBMC cells were stained with purified CPTC-PLXNB2-2 monoclonal antibody (solid green) and then fixed. PBMC cells were stained with concentration-matched mouse IgG2a isotype control antibody (dashed green) and then fixed. A BV421 conjugated goat anti-mouse IgG (minimal x-reactivity) was used as a secondary antibody. The fluorescence histograms were derived from gated events with forward (FSC) and side (SSC) scatter characteristics of viable monocytes. All data were analyzed using FlowJo. Click image to enlarge

CPTC-PLXNB2-2 (Flow Cytometry)

Result: Positive

Flow cytometric analysis of human PLXNB2 cell surface expression on human peripheral blood mononuclear cells (PBMC). PBMC cells were stained with purified CPTC-PLXNB2-2 monoclonal antibody (solid green) and then fixed. PBMC cells were stained with concentration-matched mouse IgG2a isotype control antibody (dashed green) and then fixed. A BV421 conjugated goat anti-mouse IgG (minimal x-reactivity) was used as a secondary antibody. The fluorescence histograms were derived from gated events with forward (FSC) and side (SSC) scatter characteristics of viable monocytes. All data were analyzed using FlowJo.


Characterization SOP Files

  Immunofluorescence

CPTC-PLXNB2-2 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-PLXNB2-2 as primary antibody against PBMC (human mononuclear cells). CPTC-PLXNB2-2 ab shows no PLXNB2 localization in PBMC.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-PLXNB2-2 as primary mouse antibody against BSA-conjugated PLXNB2-3 peptide (TDSREAFEAYTDHATYC, red) and BSA-conjugated PLXNB2-4 peptide (CRDIFYKPFHGDIQCGGHAPGSSKS, blue) coated on the plate and detected using goat anti-mouse antibody and TMB. Click image to enlarge

CPTC-PLXNB2-2 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-PLXNB2-2 as primary mouse antibody against BSA-conjugated PLXNB2-3 peptide (TDSREAFEAYTDHATYC, red) and BSA-conjugated PLXNB2-4 peptide (CRDIFYKPFHGDIQCGGHAPGSSKS, blue) coated on the plate and detected using goat anti-mouse antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PLXNB2-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image CPTC-PLNXNB2-2 was tested in Simple Western against three different proteins: PLXNB1, PLNXB2 and PLNXB3. In the upper panel, the antibody recognized specifically PLXNB2. In the bottom panel, the same His-tagged recombinant protein were tested using an anti-His tag antibody. and all three proteins were detected. Click image to enlarge

CPTC-PLXNB2-2 Simple Western (Recombinant Protein)

Result: Positive

CPTC-PLNXNB2-2 was tested in Simple Western against three different proteins: PLXNB1, PLNXB2 and PLNXB3. In the upper panel, the antibody recognized specifically PLXNB2. In the bottom panel, the same His-tagged recombinant protein were tested using an anti-His tag antibody. and all three proteins were detected.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image CPTC-PLXNB2-2 was tested in Western Blot against three different His-tagged recombinant proteins: PLXNB1, PLXNB2 and PLXNB3. The antibody specifically recognizes PLXNB2 (orange color, as results of overlapping pink and green signal). The same blot was probed with an anti-His tag antibody, and all the three protein were detected (green signal). Click image to enlarge

CPTC-PLXNB2-2 Western Blot (Recombinant Protein)

Result: Positive

CPTC-PLXNB2-2 was tested in Western Blot against three different His-tagged recombinant proteins: PLXNB1, PLXNB2 and PLXNB3. The antibody specifically recognizes PLXNB2 (orange color, as results of overlapping pink and green signal). The same blot was probed with an anti-His tag antibody, and all the three protein were detected (green signal).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PLXNB2-2 Western Blot (Tissue Lysates)

Result: Negative

CPTC-PLNXB2-2 was used to probe 4 different tissue lysates in Simple Western. The tested lysates were: Brain, Kidney, Liver, and Plasma. The target protein was not detected in any of the tested lysates.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image CPTC-PLXNB2-3 was used to probe four different tissue lysates. The tissues were brain, kidney, liver and plasma. The target protein was detected in brain, (weak signal), kidney and liver, but not in plasma. The same blot was probed with an anti-Vinculin antibody, and the housekeeping protein was detected only in brain, kidney and liver. Click image to enlarge

CPTC-PLXNB2-2 Western Blot (Tissue Lysates)

Result: Positive

CPTC-PLXNB2-3 was used to probe four different tissue lysates. The tissues were brain, kidney, liver and plasma. The target protein was detected in brain, (weak signal), kidney and liver, but not in plasma. The same blot was probed with an anti-Vinculin antibody, and the housekeeping protein was detected only in brain, kidney and liver.


Characterization SOP Files

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Antibody: CPTC-PLXNB2-3
Background

Catalog Number:

CPTC-PLXNB2-3

Target Antigen:

Plexin B2 Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-PLXNB2-3 and BSA-conjugated peptide PLXNB2-4, (CRDIFYKPFHGDIQCGGHAPGSSKS), were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled onto AR2G biosensors. CPTC-PLXNB2-3 mouse antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM, and 0.25 nM. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data was analyzed globally using a 1:2 bivalent fitting model. Click image to enlarge

CPTC-PLXNB2-3 Affinity and Kinetics (Bio-Layer Interferometry)

Result: High Binding

Affinity and binding kinetics of CPTC-PLXNB2-3 and BSA-conjugated peptide PLXNB2-4, (CRDIFYKPFHGDIQCGGHAPGSSKS), were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled onto AR2G biosensors. CPTC-PLXNB2-3 mouse antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM, and 0.25 nM. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data was analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-PLXNB2-3 antibody and BSA-conjugated peptide, PLXNB2-4 (CRDIFYKPFHGDIQCGGHAPGSSKS) were measured using surface plasmon resonance. BSA-peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-PLXNB2-3, mouse antibody, was used as analyte and was titrated at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1 nM. Click image to enlarge

CPTC-PLXNB2-3 Affinity and Kinetics (Surface Plasmon Resonance)

Result: High Binding

Affinity and binding kinetics of CPTC-PLXNB2-3 antibody and BSA-conjugated peptide, PLXNB2-4 (CRDIFYKPFHGDIQCGGHAPGSSKS) were measured using surface plasmon resonance. BSA-peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-PLXNB2-3, mouse antibody, was used as analyte and was titrated at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1 nM.


Characterization SOP Files

  Flow Cytometry
Click to enlarge image Flow cytometric analysis of human PLXNB2 cell surface expression on human peripheral blood mononuclear cells (PBMC). PBMC cells were stained with purified CPTC-PLXNB2-3 monoclonal antibody (solid green) and then fixed. PBMC cells were stained with concentration-matched mouse IgG1 isotype control antibody (dashed green) and then fixed. A BV421 conjugated goat anti-mouse IgG (minimal x-reactivity) was used as a secondary antibody. The fluorescence histograms were derived from gated events with forward (FSC) and side (SSC) scatter characteristics of viable monocytes. All data were analyzed using FlowJo. Click image to enlarge

CPTC-PLXNB2-3 (Flow Cytometry)

Result: Negative

Flow cytometric analysis of human PLXNB2 cell surface expression on human peripheral blood mononuclear cells (PBMC). PBMC cells were stained with purified CPTC-PLXNB2-3 monoclonal antibody (solid green) and then fixed. PBMC cells were stained with concentration-matched mouse IgG1 isotype control antibody (dashed green) and then fixed. A BV421 conjugated goat anti-mouse IgG (minimal x-reactivity) was used as a secondary antibody. The fluorescence histograms were derived from gated events with forward (FSC) and side (SSC) scatter characteristics of viable monocytes. All data were analyzed using FlowJo.


Characterization SOP Files

  Immunofluorescence

CPTC-PLXNB2-3 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-PLXNB2-3 as primary antibody against PBMC (human mononuclear cells). CPTC-PLXNB2-3 ab shows no PLXNB2 localization in PBMC.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-PLXNB2-3 as primary mouse antibody against BSA-conjugated PLXNB2-3 peptide (TDSREAFEAYTDHATYC, red) and BSA-conjugated PLXNB2-4 peptide (CRDIFYKPFHGDIQCGGHAPGSSKS, blue) coated on the plate and detected using goat anti-mouse antibody and TMB. Click image to enlarge

CPTC-PLXNB2-3 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-PLXNB2-3 as primary mouse antibody against BSA-conjugated PLXNB2-3 peptide (TDSREAFEAYTDHATYC, red) and BSA-conjugated PLXNB2-4 peptide (CRDIFYKPFHGDIQCGGHAPGSSKS, blue) coated on the plate and detected using goat anti-mouse antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PLXNB2-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image CPTC-PLNXNB2-3 was tested in Simple Western against three different proteins: PLXNB1, PLNXB2 and PLNXB3. In the upper panel, the antibody recognized specifically PLXNB2. In the bottom panel, the same His-tagged recombinant protein were tested using an anti-His tag antibody. and all three proteins were detected. Click image to enlarge

CPTC-PLNXNB2-3 Simple Western (recombinant Protein)

Result: Positive

CPTC-PLNXNB2-3 was tested in Simple Western against three different proteins: PLXNB1, PLNXB2 and PLNXB3. In the upper panel, the antibody recognized specifically PLXNB2. In the bottom panel, the same His-tagged recombinant protein were tested using an anti-His tag antibody. and all three proteins were detected.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image CPTC-PLXNB2-3 was tested in Western Blot against three different His-tagged recombinant proteins: PLXNB1, PLXNB2 and PLXNB3. The antibody specifically recognizes PLXNB2 (orange color, as results of overlapping pink and green signal). The same blot was probed with an anti-His tag antibody, and all the three protein were detected (green signal). Click image to enlarge

CPTC-PLXNB2-3 Western Blot (Recombinant Protein)

Result: Positive

CPTC-PLXNB2-3 was tested in Western Blot against three different His-tagged recombinant proteins: PLXNB1, PLXNB2 and PLXNB3. The antibody specifically recognizes PLXNB2 (orange color, as results of overlapping pink and green signal). The same blot was probed with an anti-His tag antibody, and all the three protein were detected (green signal).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PLXNB2-3 Simple Western (Tissue Lysates)

Result: Negative

CPTC-PLNXB2-3 was used to probe 4 different tissue lysates in Simple Western. The tested lysates were: Brain, Kidney, Liver, and Plasma. The target protein was not detected in any of the tested lysates.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PLXNB2-3 Western Blot (Cell Lysates)

Result: Negative

CPTC-PLXNB2-3 was used to probe six different cell lysates. The cell lysates were HCC2998, NCI H322M, SF-539, HCT-15, MOLT-4 and CCRF-CEM. The target was not detected in any of the tested cell lysates. The same blot was probed with an anti-Vinculin antibody, and all the cell lysates showed expression of the housekeeping protein at different levels.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image CPTC-PLXNB2-3 was used to probe four different tissue lysates. The tissues were brain, kidney, liver and plasma. The target protein was detected in brain, (weak signal), and kidney. It was presumably detected in liver, but not in plasma. The same blot was probed with an anti-Vinculin antibody, and the housekeeping protein was detected only in brain, kidney and liver. Click image to enlarge

CPTC-PLXNB2-3 Western Blot (Tissue Lysates)

Result: Positive

CPTC-PLXNB2-3 was used to probe four different tissue lysates. The tissues were brain, kidney, liver and plasma. The target protein was detected in brain, (weak signal), and kidney. It was presumably detected in liver, but not in plasma. The same blot was probed with an anti-Vinculin antibody, and the housekeeping protein was detected only in brain, kidney and liver.


Characterization SOP Files

[top]

Antigen: Plexin B2 Peptide 1

Background

NCI Identification Number:

00552

Antigen Name:

Plexin B2 Peptide 1

CPTC Name:

CPTC-PLXNB2 Peptide 1

Aliases:

Plexin B2; MM1; KIAA0315; PLEXB2; Ferroptosis-Associated LncRNA; Plexin-B2; LncFAL; DJ402G11.3; Nbla00445

Function:

Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.PLXNB2 (Plexin B2) is a Protein Coding gene. Diseases associated with PLXNB2 include Spinocerebellar Ataxia, X-Linked 5 and Walker-Warburg Syndrome. Gene Ontology (GO) annotations related to this gene include signaling receptor activity and semaphorin receptor activity. An important paralog of this gene is PLXNB1.Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling (By similarity). Plays a role in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development (By similarity). Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248' (By similarity). Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development (By similarity). Regulates the migration of cerebellar granule cells in the developing brain (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12183458). Plays a role in axon guidance, invasive growth and cell migration (PubMed:15184888). May modulate the activity of RAC1 and CDC42.

Chromosomal Localization:

22q13.33

Accession Number:

UniProt Accession Number:

B2RXS4

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TDSREAFEAYTDHATYC and CRDIFYKPFHGDIQ(Cam)GGHAPGSSK
S

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1870

Last Updated:

12/08/2023

Links

Characterization Data

SOPs

No SOPs available.

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