PRINT

PAX3 And PAX7 Binding Protein 1 Peptide 1

Antibody: CPTC-PAXBP1-1
Background

Catalog Number:

CPTC-PAXBP1-1

RRID:

NA

Target Antigen:

PAX3 And PAX7 Binding Protein 1 Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

02/02/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-PAXBP1-1 and BSA-conjugated peptide “LKPRKRPRENKEVPC" were measured using biolayer interferometry. BSA conjugated peptide was immobilized onto AR2G biosensors using standard amine coupling. CPTC-PAXBP1-1 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 1 nM, and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. Data was analyzed globally. Click image to enlarge

CPTC-PAXBP1-1 Affinity and Kinetics (Bio-Layer Interferometry)

Result: High Binding

The affinity and binding kinetics of CPTC-PAXBP1-1 and BSA-conjugated peptide “LKPRKRPRENKEVPC" were measured using biolayer interferometry. BSA conjugated peptide was immobilized onto AR2G biosensors using standard amine coupling. CPTC-PAXBP1-1 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 1 nM, and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. Data was analyzed globally.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-PAXBP1-1 and BSA-conjugated peptide, “LKPRKRPRENKEVPC”, were measured using surface plasmon resonance. BSA conjugated peptide was amine coupled onto a Series S CM5 biosensor chip and CPTC-PAXBP1-1 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, and 1.0 nM was used as analyte. Kinetic constant Kd is outside the limits that can be measured by the instrument. The kinetic constants cannot be uniquely determined. Try to extend the dissociation time. Binding data were double-referenced and analyzed globally. Click image to enlarge

CPTC-PAXBP1-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: High Binding

Affinity and binding kinetics of CPTC-PAXBP1-1 and BSA-conjugated peptide, “LKPRKRPRENKEVPC”, were measured using surface plasmon resonance. BSA conjugated peptide was amine coupled onto a Series S CM5 biosensor chip and CPTC-PAXBP1-1 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, and 1.0 nM was used as analyte. Kinetic constant Kd is outside the limits that can be measured by the instrument. The kinetic constants cannot be uniquely determined. Try to extend the dissociation time. Binding data were double-referenced and analyzed globally.


Characterization SOP Files

  IHC Tissue

CPTC-PAXBP1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immunofluorescence

CPTC-PAXBP1-1 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-PAXBP1-1 as primary antibody against EL4 and Jurkat cells shows no localization of PAXBP1 protein.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-PAXBP1-1 against BSA-conjugated peptide, "LKPRKRPRENKEVPC". Click image to enlarge

CPTC-PAXBP1-1 Indirect ELISA 

Result: High Binding

Indirect ELISA using CPTC-PAXBP1-1 against BSA-conjugated peptide, "LKPRKRPRENKEVPC".


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PAXBP1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PAXBP1-1 as primary antibody against human PAXBP1 recombinant protein (expected MW is about 70.6 KDa). The antibody CPTC-PAXBP1-1 recognizes the human recombinant protein. Click image to enlarge

CPTC-PAXBP1-1 Simple Western (recombinant protein)

Result: Positive

Western Blot using CPTC-PAXBP1-1 as primary antibody against human PAXBP1 recombinant protein (expected MW is about 70.6 KDa). The antibody CPTC-PAXBP1-1 recognizes the human recombinant protein.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-PAXBP1-1 as primary antibody against human PAXBP1 recombinant protein. Expected MW is about 70.6 kDa. The antibody CPTC-PAXBP1-1 recognizes the human recombinant protein. Click image to enlarge

CPTC-PAXBP1-1 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-PAXBP1-1 as primary antibody against human PAXBP1 recombinant protein. Expected MW is about 70.6 kDa. The antibody CPTC-PAXBP1-1 recognizes the human recombinant protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-PAXBP1-1 as a primary antibody against cell lysates. Relative expression of total PAXBP1 in EL4 cells (A). Percentage of cells that express PAXBP1 (B). Average expression of PAXBP1 protein per cell (C). All data is normalized to β-tubulin expression. PAXBP1 protein expression was not detected in Jurkat cell lysate. Click image to enlarge

CPTC-PAXBP1-1 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-PAXBP1-1 as a primary antibody against cell lysates. Relative expression of total PAXBP1 in EL4 cells (A). Percentage of cells that express PAXBP1 (B). Average expression of PAXBP1 protein per cell (C). All data is normalized to β-tubulin expression. PAXBP1 protein expression was not detected in Jurkat cell lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-PAXBP1-1 as primary antibody against human cell lysates CCRF-CEM, K-52, MOLT-, SR, Jurkat and mouse cell lysates CHI2.LX and EL4 (expected MW is about 105 KDa). The antibody CPTC-PAXBP1-1 does not recognize the endogenous protein in any of the tested lysates. Cell lines were also tested for GAPDH. Click image to enlarge

CPTC-PAXBP1-1 Simple Western (lysates)

Result: Negative

Western Blot using CPTC-PAXBP1-1 as primary antibody against human cell lysates CCRF-CEM, K-52, MOLT-, SR, Jurkat and mouse cell lysates CHI2.LX and EL4 (expected MW is about 105 KDa). The antibody CPTC-PAXBP1-1 does not recognize the endogenous protein in any of the tested lysates. Cell lines were also tested for GAPDH.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PAXBP1-1 as primary antibody against human cell lysates CCRF-CEM, K-52, MOLT-, SR and Jurkat whole cell lysates. Expected MW is about 105 kDa. The antibody CPTC-PAXBP1-1 does not recognize the endogenous protein in any of the tested lysates. Click image to enlarge

CPTC-PAXBP1-1 Western Blot (Cell Lysates)

Result: Negative

Western blot using CPTC-PAXBP1-1 as primary antibody against human cell lysates CCRF-CEM, K-52, MOLT-, SR and Jurkat whole cell lysates. Expected MW is about 105 kDa. The antibody CPTC-PAXBP1-1 does not recognize the endogenous protein in any of the tested lysates.


Characterization SOP Files

[top]

Antibody: CPTC-PAXBP1-2
Background

Catalog Number:

CPTC-PAXBP1-2

RRID:

NA

Target Antigen:

PAX3 And PAX7 Binding Protein 1 Peptide 1

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

02/02/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-PAXBP1-2 and BSA-conjugated peptide “LKPRKRPRENKEVPC" were measured using biolayer interferometry. CPTC-PAXBP1-2 antibody was captured onto Protein G biosensors. BSA-conjugated peptide, 1024 nM, 256 nM, 64 nM, 16 nM, and 1 nM, were used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. Click image to enlarge

CPTC-PAXBP1-2 Affinity and Kinetics (BioLayer Interferometry)

Result: High Binding

The affinity and binding kinetics of CPTC-PAXBP1-2 and BSA-conjugated peptide “LKPRKRPRENKEVPC" were measured using biolayer interferometry. CPTC-PAXBP1-2 antibody was captured onto Protein G biosensors. BSA-conjugated peptide, 1024 nM, 256 nM, 64 nM, 16 nM, and 1 nM, were used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-PAXBP1-2 and BSA-conjugated peptide, “LKPRKRPRENKEVPC“, using surface plasmon resonance. CPTC-PAXBP1-2 antibody was captured onto a Series S Protein G biosensor chip and BSA-conjugated peptide, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, and 1.0 nM was used as analyte. Binding data were double-referenced and analyzed globally. Click image to enlarge

CPTC-PAXBP1-2 Affinity and Kinetics (Surface Plasmon Resonance)

Result: High Binding

Affinity and binding kinetics of CPTC-PAXBP1-2 and BSA-conjugated peptide, “LKPRKRPRENKEVPC“, using surface plasmon resonance. CPTC-PAXBP1-2 antibody was captured onto a Series S Protein G biosensor chip and BSA-conjugated peptide, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, and 1.0 nM was used as analyte. Binding data were double-referenced and analyzed globally.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-PAXBP1-2 and BSA-conjugated peptide, “LKPRKRPRENKEVPC“, were measured using surface plasmon resonance. CPTC-PAXBP1-2 antibody was captured onto a Series S Protein G biosensor chip and BSA-conjugated peptide, 256 nM, 64 nM, 16 nM, 4 nM, and 1.0 nM, was used as analyte. Binding data were double-referenced and analyzed globally. Click image to enlarge

CPTC-PAXBP1-2 Affinity and Kinetics (Surface Plasmon Resonance)

Result: High Binding

Affinity and binding kinetics of CPTC-PAXBP1-2 and BSA-conjugated peptide, “LKPRKRPRENKEVPC“, were measured using surface plasmon resonance. CPTC-PAXBP1-2 antibody was captured onto a Series S Protein G biosensor chip and BSA-conjugated peptide, 256 nM, 64 nM, 16 nM, 4 nM, and 1.0 nM, was used as analyte. Binding data were double-referenced and analyzed globally.


Characterization SOP Files

  IHC Tissue

CPTC-PAXBP1-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immunofluorescence

CPTC-PAXBP1-2 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-PAXBP1-2 as primary antibody against EL4 and Jurkat cells shows no localization of PAXBP1 protein.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-PAXBP1-2 against BSA-conjugated peptide, "LKPRKRPRENKEVPC". Click image to enlarge

CPTC-PAXBP1-2 Indirect ELISA 

Result: High Binding

Indirect ELISA using CPTC-PAXBP1-2 against BSA-conjugated peptide, "LKPRKRPRENKEVPC".


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PAXBP1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-PAXBP1-2 as primary antibody against human PAXBP1 recombinant protein. Expected MW is about 70.6 kDa. The antibody CPTC-PAXBP1-2 recognizes the human recombinant protein. Click image to enlarge

CPTC-PAXBP1-2 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-PAXBP1-2 as primary antibody against human PAXBP1 recombinant protein.
Expected MW is about 70.6 kDa. The antibody CPTC-PAXBP1-2 recognizes the human recombinant protein.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PAXBP1-2 as primary antibody against human PAXBP1 recombinant protein (expected MW is about 70.6 KDa). The antibody CPTC-PAXBP1-2 recognizes the human recombinant protein. Click image to enlarge

CPTC-PAXBP1-2 Simple Western (recombinant protein)

Result: Negative

Western Blot using CPTC-PAXBP1-2 as primary antibody against human PAXBP1 recombinant protein (expected MW is about 70.6 KDa). The antibody CPTC-PAXBP1-2 recognizes the human recombinant protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PAXBP1-2 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-PAXBP1-2 as a primary antibody against EL4 and Jurkat cell lysates. PAXBP1 protein expression was not detected.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-PAXBP1-1 as primary antibody against human cell lysates CCRF-CEM, K-52, MOLT-, SR, Jurkat and mouse cell lysates CHI2.LX and EL4 (expected MW is about 105 KDa). The antibody CPTC-PAXBP1-1 does not recognize the endogenous protein in any of the tested lysates. Cell lines were also tested for GAPDH. Click image to enlarge

CPTC-PAXBP1-2 Simple Western (lysates)

Result: Negative

Western Blot using CPTC-PAXBP1-1 as primary antibody against human cell lysates CCRF-CEM, K-52, MOLT-, SR, Jurkat and mouse cell lysates CHI2.LX and EL4 (expected MW is about 105 KDa). The antibody CPTC-PAXBP1-1 does not recognize the endogenous protein in any of the tested lysates. Cell lines were also tested for GAPDH.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PAXBP1-2 as primary antibody against human cell lysates CCRF-CEM, K-52, MOLT-, SR and Jurkat whole cell lysates. Expected MW is about 105 kDa. The antibody CPTC-PAXBP1-2 does not recognize the endogenous protein in any of the tested lysates. Click image to enlarge

CPTC-PAXBP1-2 Western Blot (Cell Lysates)

Result: Negative

Western blot using CPTC-PAXBP1-2 as primary antibody against human cell lysates CCRF-CEM, K-52, MOLT-, SR and Jurkat whole cell lysates. Expected MW is about 105 kDa. The antibody CPTC-PAXBP1-2 does not recognize the endogenous protein in any of the tested lysates.


Characterization SOP Files

[top]

Antigen: PAX3 And PAX7 Binding Protein 1 Peptide 1
Background

NCI Identification Number:

00515

Antigen Name:

PAX3 And PAX7 Binding Protein 1 Peptide 1

CPTC Name:

CPTC-PAXBP1 Peptide 1

Aliases:

PAX3 And PAX7 Binding Protein 1; GCFC; GC-Rich Sequence DNA-Binding Factor 1; C21orf66; FSAP105; GCFC1; Functional Spliceosome-Associated Protein 105; GC-Rich Sequence DNA-Binding Factor Candidate; PAX3- And PAX7-Binding Protein 1; Chromosome 21 Open Reading Frame 66; BM020

Function:

This gene encodes a protein that may bind to GC-rich DNA sequences, which suggests its involvement in the regulation of transcription. Alternative splicing of this gene results in multiple transcript variants.PAXBP1 (PAX3 And PAX7 Binding Protein 1) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and RNA polymerase II cis-regulatory region sequence-specific DNA binding. An important paralog of this gene is GCFC2.Adapter protein linking the transcription factors PAX3 and PAX7 to the histone methylation machinery and involved in myogenesis. Associates with a histone methyltransferase complex that specifically mediates dimethylation and trimethylation of 'Lys-4' of histone H3. Mediates the recruitment of that complex to the transcription factors PAX3 and PAX7 on chromatin to regulate the expression of genes involved in muscle progenitor cells proliferation including ID3 and CDC20 (By similarity).

Chromosomal Localization:

21q22.11

Accession Number:

NP_057715.2

UniProt Accession Number:

Q9Y5B6

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LKPRKRPRENKEVP

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1540

Last Updated:

03/29/2022

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]