PRINT

Nuclear Mitotic Apparatus Protein 1 Peptide 1

Antibody: CPTC-NUMA1-1
Background

Catalog Number:

CPTC-NUMA1-1

Target Antigen:

Nuclear Mitotic Apparatus Protein 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

04/19/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (251.9 KB)

CPTC-NUMA1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Download Immuno-histochemistry of CPTC-NUMA1-1 for NCI60 Cell Line Array with titer 1:1250 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) (87.4 KB)

CPTC-NUMA1-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-NUMA1-1 for NCI60 Cell Line Array with titer 1:1250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-NUMA1-1. Titer: 1:1250 Click image to enlarge

CPTC-NUMA1-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-NUMA1-1. Titer: 1:1250


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-NUMA1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5920) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-NUMA1-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-NUMA1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5920)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-NUMA1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line. Click image to enlarge

CPTC-NUMA1-1 automated western blot (cell lysate)

Result: Positive

Automated western blot using CPTC-NUMA1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-NUMA1-1 as primary antibody against LCL57 cell lysate. Cell lysate was irradiated with 10 Gy as shown in the ‘+’ indicated lane. Non-irradiated cell lysate was treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lane. Molecular weight standards are also included. Western blot was developed using enhanced chemiluminescence. Click image to enlarge

CPTC-NUMA1-1 western blot (cell lysate)

Result: Positive

Western blot using CPTC-NUMA1-1 as primary antibody against LCL57 cell lysate. Cell lysate was irradiated with 10 Gy as shown in the ‘+’ indicated lane. Non-irradiated cell lysate was treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lane. Molecular weight standards are also included. Western blot was developed using enhanced chemiluminescence.


Characterization SOP Files

[top]

Antigen: Nuclear Mitotic Apparatus Protein 1 Peptide 1
Background

NCI Identification Number:

00291

Antigen Name:

Nuclear Mitotic Apparatus Protein 1 Peptide 1

CPTC Name:

CPTC-NUMA1 Peptide 1

Aliases:

Nuclear Mitotic Apparatus Protein 1; Nuclear Matrix Protein-22; SP-H Antigen; NMP-22; NUMA; Nuclear Mitotic Apparatus Protein; Structural Nuclear Protein; NuMA Protein; NMP22

Function:

This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants.NUMA1 (Nuclear Mitotic Apparatus Protein 1) is a Protein Coding gene. Diseases associated with NUMA1 include Acute Promyelocytic Leukemia and Pyuria. Among its related pathways are Apoptosis and survival Caspase cascade and Cell Cycle, Mitotic. Gene Ontology (GO) annotations related to this gene include nucleotide binding and microtubule binding. An important paralog of this gene is GOLGA3.Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed:7769006, PubMed:17172455, PubMed:19255246, PubMed:24996901, PubMed:26195665, PubMed:27462074). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed:12445386, PubMed:11956313). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901, PubMed:26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed:23027904, PubMed:22327364, PubMed:23921553). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PubMed:22327364, PubMed:23921553, PubMed:24996901, PubMed:24371089). Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PubMed:24996901, PubMed:24371089). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed:21816348). Plays a role in mitotic MT aster assembly (PubMed:11163243, PubMed:11229403, PubMed:12445386). Involved in anastral spindle assembly (PubMed:25657325). Positively regulates TNKS protein localization to spindle poles in mitosis (PubMed:16076287). Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PubMed:10075938). Required for epidermal differentiation and hair follicle morphogenesis (By similarity).

Chromosomal Localization:

11q13.4

Accession Number:

na

UniProt Accession Number:

Q14980

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CLSQLEEHLS(ph)QLQDNPPQEK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2200

Last Updated:

10/22/2019

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]