Background
Catalog Number:
CPTC-NF1-1
RRID:
AB_2920914
Target Antigen:
Neurofibromin 1
Isotype:
IgG3
Species:
Mouse Monoclonal Antibody
Last Updated:
10/20/2023
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-NF1-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (455.6 KB)
CPTC-NF1-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IP
CPTC-NF1-1 IP-WB
Result: Negative
IP-WB performed with CPTC-NF1-1 as capture antibody against recombinant protein and MCF7 lysate. Eluates were screened in WB using antibody CPTC-NF1-1 as detection antibody. This antibody is not suitable for use in this application.
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (455.6 KB)
CPTC-NF1-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Background
Catalog Number:
CPTC-NF1-2
RRID:
AB_2920915
Target Antigen:
Neurofibromin 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
10/19/2023
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-NF1-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (390.6 KB)
CPTC-NF1-2 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IP
CPTC-NF1-2 IP-WB
Result: Negative
IP-WB performed with CPTC-NF1-2 as capture antibody against recombinant protein and MCF7 lysate. Eluates were screened in WB using antibody CPTC-NF1-1 as detection antibody. This antibody is not suitable for use in this application.
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (390.6 KB)
CPTC-NF1-2 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Background
Catalog Number:
CPTC-NF1-3
RRID:
AB_2920916
Target Antigen:
Neurofibromin 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
10/20/2023
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-NF1-3
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (379.1 KB)
CPTC-NF1-3 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (379.1 KB)
CPTC-NF1-3 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Background
NCI Identification Number:
00489
Antigen Name:
Neurofibromin 1
CPTC Name:
CPTC-NF1
Aliases:
Neurofibromin 1; Neurofibromatosis-Related Protein NF-1; Neurofibromin; Von Recklinghausen Disease; Truncated Neurofibromin 1; Neurofibromatosis 1; Neurofibromatosis; Watson Disease; NFNS; VRNF; WSS; NF1
Function:
This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.NF1 (Neurofibromin 1) is a Protein Coding gene. Diseases associated with NF1 include Neurofibromatosis-Noonan Syndrome and Neurofibromatosis, Type I. Among its related pathways are RET signaling and Signaling by GPCR. Gene Ontology (GO) annotations related to this gene include binding and phosphatidylcholine binding. An important paralog of this gene is RASA3.Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
Chromosomal Localization:
17q11.2
Expression System:
Baculovirus
Accession Number:
NP_001035957.1
UniProt Accession Number:
P21359
DNA Source:
N/A
Immunogen:
Recombinant Full Length Protein
Vector Name:
xx
Extinction Coefficient:
Buffers:
Expressed Sequence:
MGSGSHHHHHHRSSITSLYKKVGENLYFQGAAHRPVEWVQAVVSRFDEQL
PIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIF
GEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFL
HTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDN
VDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFW
NWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQI
ILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAA
IACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPA
DVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNS
ALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEK
VTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGS
TAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFW
EISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSS
CHFLLFYGVGCDIPSSGNTSQMSMDHEELLRTPGASLRKGKGNSSMDSAA
GCSGTPPICRQAQTKLEVALYMFLWNPDTEAVLVAMSCFRHLCEEADIRC
GVDEVSVHNLLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTA
GNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVS
GGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVS
ERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLV
GLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNL
LDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEV
MMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQA
SMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVE
DESAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHK
DLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGEL
PIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSM
QTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEV
DPTRLEPSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQV
VSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLM
SKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDAV
NHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATL
LAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSI
FYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIV
VDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKY
HERLLTGLKGSKRLVFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHN
ALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEIC
LVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQH
TKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQL
LETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELK
HLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSI
QAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGN
VKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNN
SLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFS
EETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSY
ERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNP
SLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIE
ATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGTALLEQNL
HTLDSLRIFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVG
HLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAAL
LTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKETQ
PWSSPKGSEGYLAATYPTVGQTSPRARKSMSLDMGQPSQANTKKLLGTRK
SFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPH
LRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEY
LAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHE
ESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTY
LPGIDEETSEESLLTPTSPYPPALQSQLSITANLNLSNSMTSLATSQHSP
GIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV
Native Sequence:
Calculated Isoelectric Point:
7.02
Molecular Weight:
320316
Last Updated:
06/23/2021
Links
- EnsEMBL - EnsEMBL NF1
- Entrez - Entrez NF1
- Uniprot - Uniprot P21359
Characterization Data
SOPs
No SOPs available.
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