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Non-SMC Condensin II Complex Subunit H2 Peptide 1

Antibody: CPTC-NCAPH2-1
Background

Catalog Number:

CPTC-NCAPH2-1

Target Antigen:

Non-SMC Condensin II Complex Subunit H2 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (226.0 KB)

CPTC-NCAPH2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Click to enlarge image Immuno-histochemistry of CPTC-NCAPH2-1 for NCI60 Cell Line Array with titer 1:50 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) Click image to enlarge

CPTC-NCAPH2-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-NCAPH2-1 for NCI60 Cell Line Array with titer 1:50
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Download Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-NCAPH2-1. Titer: 1:50 (3.5 MB)

CPTC-NCAPH2-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-NCAPH2-1. Titer: 1:50


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-NCAPH2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5922) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-NCAPH2-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-NCAPH2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5922)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-NCAPH2-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line. Click image to enlarge

CPTC-NCAPH2-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-NCAPH2-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line.


Characterization SOP Files

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Antigen: Non-SMC Condensin II Complex Subunit H2 Peptide 1
Background

NCI Identification Number:

00302

Antigen Name:

Non-SMC Condensin II Complex Subunit H2 Peptide 1

CPTC Name:

CPTC-NCAPH2 Peptide 1

Aliases:

Non-SMC Condensin II Complex Subunit H2; CAP-H2 Subunit Of The Condensin II Complex; Chromosome-Associated Protein H2; Condensin-2 Complex Subunit H2; Kleisin Beta; CAPH2; Non-SMC Condensin II Complex, Subunit H2; CTA-384D8.36; Kleisin-Beta; HCAP-H2

Function:

This gene encodes one of the non-SMC subunits of the condensin II complex. This complex plays an essential role in mitotic chromosome assembly. Alternate splicing of this gene results in multiple transcript variants.NCAPH2 (Non-SMC Condensin II Complex Subunit H2) is a Protein Coding gene. Diseases associated with NCAPH2 include Myopia 6 and Mitochondrial Dna Depletion Syndrome 1. Among its related pathways are Cell Cycle, Mitotic and Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3.Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture (PubMed:14532007). May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of chromatin bridges at anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (By similarity). Seems to have lineage-specific role in T-cell development.

Chromosomal Localization:

22q13.33

Accession Number:

NP_055366.3

UniProt Accession Number:

Q6IBW4

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CFVQETELS(ph)QR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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