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Nanog Homeobox Peptide 2

Antibody: CPTC-NANOG-2
Background

Catalog Number:

CPTC-NANOG-2

RRID:

AB_2827838

Target Antigen:

Nanog Homeobox Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

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Characterization Data [Compare Characterization Data]
  IP
Download Immuno-precipitation data obtained incubating CPTC-NANOG-2 and the recombinant NANOG (aa1-305, expected, MW ~38KDa) (152.5 KB)

CPTC-NANOG-2 IPMS

Result: Positive

Immuno-precipitation data obtained incubating CPTC-NANOG-2 and the recombinant NANOG (aa1-305, expected, MW ~38KDa)


Characterization SOP Files

  Immunofluorescence

CPTC-NANOG-2 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF7 cells using CPTC-NANOG-2 antibody. NANOG protein expression was not detected.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-NANOG-2 as primary antibody against recombinant NANOG protein (amino acids 1-305). Click image to enlarge

CPTC-NANOG-2 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-NANOG-2 as primary antibody against recombinant NANOG protein (amino acids 1-305).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NANOG-2 as primary antibody against recombinant NANOG protein (lane 2) with expected MW of 37.3 KDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-NANOG-2 recombinant protein WB

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-NANOG-2 as primary antibody against recombinant NANOG protein (lane 2) with expected MW of 37.3 KDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-NANOG-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 34.6 KDa. Presumed positive for A549, H226, Jurkat and MCF7. Negative for HeLa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-NANOG-2 Endogenous Western Blot

Result: Positive

Western Blot using CPTC-NANOG-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 34.6 KDa. Presumed positive for A549, H226, Jurkat and MCF7. Negative for HeLa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-NANOG-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 34.6 KDa. Presumed positive for A549, H226, Jurkat and MCF7. Negative for HeLa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-NANOG-2 Endogenous Automated Western Blot

Result: Positive

Automated Western Blot using CPTC-NANOG-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 34.6 KDa. Presumed positive for A549, H226, Jurkat and MCF7. Negative for HeLa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Nanog Homeobox Peptide 2
Background

NCI Identification Number:

00262

Antigen Name:

Nanog Homeobox Peptide 2

CPTC Name:

CPTC-NANOG Peptide 2

Aliases:

Nanog Homeobox; Homeobox Transcription Factor Nanog; Homeobox Transcription Factor Nanog-Delta 48; Homeobox Protein NANOG; HNanog

Function:

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene.

NANOG (Nanog Homeobox) is a Protein Coding gene. Diseases associated with NANOG include Teratocarcinoma and Ovarian Serous Cystadenocarcinoma. Among its related pathways are Transcriptional Regulatory Network in Embryonic Stem Cell and Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and chromatin binding. An important paralog of this gene is NANOGP8.

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5-TAAT[GT][GT]-3 or 5-[CG][GA][CG]C[GC]ATTAN[GC]-3. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Chromosomal Localization:

12p13.31

Accession Number:

NP_079141.2

UniProt Accession Number:

Q9H9S0

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

MSSAEMPHTETVSPLPSSMDLc

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2310

Last Updated:

09/16/2019

Links

Characterization Data

SOPs

No SOPs available.

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