Background
Catalog Number:
CPTC-MUTYH-1
Target Antigen:
MutY DNA Glycosylase Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length
Purchase
- DSHB CPTC-MUTYH-1
Characterization Data [Compare Characterization Data]
NCI 60 Protein Array
CPTC-MUTYH-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-MUTYH-1 Western Blot Recombinant Protein
Result: Positive
Western blot using CPTC-MUTYH-1 as primary antibody against human mutY homolog (E. coli) (MUTYH / MYH), transcript variant alpha1, full length, with N-terminal HIS tag, recombinant protein expressed in E. coli (lane 2). Expected molecular weight - 59.9 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-MUTYH-1 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-MUTYH-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 60.1 kDa, 59.1 kDa, 58.4 kDa, 57.5 kDa, and 57.4 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-MUTYH-1 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-MUTYH-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 60.1 kDa, 59.1 kDa, 58.4 kDa, 57.5 kDa, and 57.4 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00383
Antigen Name:
MutY DNA Glycosylase Peptide 1
CPTC Name:
CPTC-MUTYH Peptide 1
Aliases:
MutY DNA Glycosylase; MYH; Adenine DNA Glycosylase; MutY Homolog; A/G-Specific Adenine DNA Glycosylase; MutY Homolog (E. Coli); MutY-Like Protein; EC 3.2.2.31; EC 3.2.2; MUTYH; HMYH
Function:
This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene.MUTYH (MutY DNA Glycosylase) is a Protein Coding gene. Diseases associated with MUTYH include Familial Adenomatous Polyposis 2 and Gastric Cancer. Among its related pathways are Telomere C-strand (Lagging Strand) Synthesis and Depurination. Gene Ontology (GO) annotations related to this gene include hydrolase activity and hydrolase activity, acting on glycosyl bonds. An important paralog of this gene is ENSG00000288208.Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.
Chromosomal Localization:
1p34.1
Accession Number:
NP_036354.1
UniProt Accession Number:
Q9UIF7
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
MGQQVLDNFFR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1210
Last Updated:
09/02/2020
Links
- CPTAC Assay Portal - EnsEMBL MUTYH
- CPTAC Assay Portal - Entrez MUTYH
- Uniprot - Uniprot Q9UIF7
Characterization Data
SOPs
No SOPs available.
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