Background
Catalog Number:
CPTC-MUC16-2
Target Antigen:
Mucin 16 Peptide 2
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/02/2024
Antigen Recognition(s):
Peptide, Endogenous
Purchase
- DSHB CPTC-MUC16-2
External Links
- Human Protein Atlas CPTC-MUC16-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (385.7 KB)
CPTC-MUC16-2 evaluation by the Human Protein Atlas
Result: Positive
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immuno-MRM
CPTC-MUC16-2 iMRM screening (synthetic peptide)
Result: Positive
iMRM screening of CPTC-MUC16-2 against synthetic peptide NTSVGPLYSGC[+57]R (Mucin 16 Peptide 1)
Data provided by the Carr Lab, Broad Institute
https://www.broadinstitute.org/proteomics/protocols
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (385.7 KB)
CPTC-MUC16-2 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
NCI 60 Protein Array
CPTC-MUC16-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-MUC16-2 Western Blot Cell Lysates and Cell Culture Supernatants
Result: Negative
Western blot using CPTC-MUC16-2 as primary antibody against cell culture supernatants OVCAR-3 (lane 2), OVCAR-4 (lane 3), OVCAR-8 (lane 4), and SK-OV-3 (lane 5) (Panel A) and against cell lysates OVCAR-3 (lane 2), OVCAR-4 (lane 3), OVCAR-8 (lane 4), and SK-OV-3 (lane 5) (Panel B). Molecular weight standards are also included in each panel (lane 1). Expected molecular weight > 250 kDa.
Characterization SOP Files
Background
NCI Identification Number:
00438
Antigen Name:
Mucin 16 Peptide 2
CPTC Name:
CPTC-MUC16 Peptide 2
Aliases:
Mucin 16, Cell Surface Associated; CA125; Ovarian Cancer-Related Tumor Marker CA125; Ovarian Carcinoma Antigen CA125; Mucin-16; CA125 Ovarian Cancer Antigen; Mucin-16 Short Isoform; Mucin-16 Long Isoform; FLJ14303; MUC-16; CA-125; MUC16
Function:
This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes.MUC16 (Mucin 16, Cell Surface Associated) is a Protein Coding gene. Diseases associated with MUC16 include Ovarian Cyst and Clear Cell Adenocarcinoma. Among its related pathways are Defective C1GALT1C1 causes Tn polyagglutination syndrome (TNPS) and Metabolism of proteins.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Chromosomal Localization:
19p13.2
Accession Number:
NP_078966.2
UniProt Accession Number:
Q8WXI7
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
NTSVGPLYSGCR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1320
Last Updated:
03/10/2021
Links
- EnsEMBL - EnsEMBL MUC16
- Entrez - Entrez MUC16
- Uniprot - Uniprot Q8WXI7
Characterization Data
SOPs
No SOPs available.
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