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MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 1

Antibody: CPTC-MRE11A-1
Background

Catalog Number:

CPTC-MRE11A-1

Target Antigen:

MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

08/03/2026

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on lung cancer tissue core using CPTC-MRE11A-1 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (prostate, liver, colon, endometrium, pancreas, bone marrow, breast, and kidney) and cancer tissues (breast, colon, prostate, lung, and ovarian). Click image to enlarge

CPTC-MRE11A-1 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on lung cancer tissue core using CPTC-MRE11A-1 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (prostate, liver, colon, endometrium, pancreas, bone marrow, breast, and kidney) and cancer tissues (breast, colon, prostate, lung, and ovarian).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (340.2 KB)

CPTC-MRE11A-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Download Immuno-histochemistry of CPTC-MRE11A-1 for NCI60 Cell Line Array with titer 1:250 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) (54.4 KB)

CPTC-MRE11A-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-MRE11A-1 for NCI60 Cell Line Array with titer 1:250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of pancreatic cancer showing cytoplasmic staining using Antibody CPTC-MRE11A-1. Titer: 1:250 Click image to enlarge

CPTC-MRE11A-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing cytoplasmic staining using Antibody CPTC-MRE11A-1. Titer: 1:250


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MRE11A-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5911) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-MRE11A-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-MRE11A-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5911)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MRE11A-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line. Click image to enlarge

CPTC-MRE11A-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-MRE11A-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line.


Characterization SOP Files

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Antigen: MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 1
Background

NCI Identification Number:

00316

Antigen Name:

MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 1

CPTC Name:

CPTC-MRE11A Peptide 1

Aliases:

MRE11 Homolog, Double Strand Break Repair Nuclease; MRE11A; ATLD; MRE11 Homolog A, Double Strand Break Repair Nuclease; Double-Strand Break Repair Protein MRE11A; Double-Strand Break Repair Protein MRE11; Meiotic Recombination 11 Homolog 1; Meiotic Recombination 11 Homolog A; AT-Like Disease; MRE11 Homolog 1; HNGS1; MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae); MRE11 Homolog, Double Strand Break Repair Nuclease A; MRE11 Meiotic Recombination 11-Like Protein A; MRE11 Double Strand Break Repair Nuclease A; MRE11 Meiotic Recombination 11 Homolog A; DNA Recombination And Repair Protein; Endo/Exonuclease Mre11; MRE11 Homolog A; EC 3.1.-.-; MRE11B

Function:

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.MRE11 (MRE11 Homolog, Double Strand Break Repair Nuclease) is a Protein Coding gene. Diseases associated with MRE11 include Ataxia-Telangiectasia-Like Disorder 1 and Ataxia, Early-Onset, With Oculomotor Apraxia And Hypoalbuminemia. Among its related pathways are Cell Cycle Checkpoints and DNA Damage.Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11 (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). RAD50 may be required to bind DNA ends and hold them in close proximity (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289, PubMed:30612738). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation.

Chromosomal Localization:

11q21

Accession Number:

NP_005582.1

UniProt Accession Number:

P49959

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CIMS(ph)QSQVSK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

08/31/2021

Links

Characterization Data

SOPs

No SOPs available.

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