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Myeloperoxidase Peptide 1

Antibody: CPTC-MPO-1
Background

Catalog Number:

CPTC-MPO-1

RRID:

AB_2814784

Target Antigen:

Myeloperoxidase Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

01/06/2026

Antigen Recognition(s):

Peptide, Endogenous

Purchase
External Links
Publications
Characterization Data [Compare Characterization Data]
  Flow Cytometry
Click to enlarge image Flow cytometric analysis of Myeloperoxidase (MPO) expression using CPTC-MPO-1 mouse IgG2b antibody. HL-60 cells were fixed, permeabilized, and then stained with CPTC-MPO-1 (solid green) or concentration-matched mouse IgG2b isotype control (dashed green) antibodies. HeLa cells were fixed, permeabilized, and then stained with CPTC-MPO-1 (solid blue) or concentration-matched mouse IgG2b isotype control (dashed blue) antibodies. A BV421 conjugated goat anti-mouse IgG was used as a secondary antibody. All data were analyzed using FlowJo. Click image to enlarge

CPTC-MPO-1 Flow Cytometry

Result: Uncertain

Flow cytometric analysis of Myeloperoxidase (MPO) expression using CPTC-MPO-1 mouse IgG2b antibody. HL-60 cells were fixed, permeabilized, and then stained with CPTC-MPO-1 (solid green) or concentration-matched mouse IgG2b isotype control (dashed green) antibodies. HeLa cells were fixed, permeabilized, and then stained with CPTC-MPO-1 (solid blue) or concentration-matched mouse IgG2b isotype control (dashed blue) antibodies. A BV421 conjugated goat anti-mouse IgG was used as a secondary antibody. All data were analyzed using FlowJo.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (505.4 KB)

CPTC-MPO-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining using CPTC-MPO-1 as primary antibody (pink-violet) against HeLa and HL-60 cells. HL-60 cells show MPO protein subcellular localization in the lysosome. Click image to enlarge

CPTC-MPO-1 Immunofluorescence

Result: Positive

Immunofluorescence staining using CPTC-MPO-1 as primary antibody (pink-violet) against HeLa and HL-60 cells. HL-60 cells show MPO protein subcellular localization in the lysosome.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (505.4 KB)

CPTC-MPO-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MPO-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MPO-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-MPO-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot of CPTC-MPO-1 against the over-expressed lysate of MPO. Expected MW is 83.9 KDa. The antibody detected the target protein. Click image to enlarge

CPTC-MPO-1 Western Blot (Overexpressed Cell Lysate)

Result: Positive

Western Blot of CPTC-MPO-1 against the over-expressed lysate of MPO. Expected MW is 83.9 KDa. The antibody detected the target protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-MPO-1 as a primary antibody against HeLa and HL-60 cell lysates. Relative expression of total MPO (A). Percentage of cells that express MPO (B). Average expression of MPO protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-MPO-1 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-MPO-1 as a primary antibody against HeLa and HL-60 cell lysates. Relative expression of total MPO (A). Percentage of cells that express MPO (B). Average expression of MPO protein per cell (C). All data is normalized to β-tubulin expression.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MPO-1 as primary antibody against the whole lysates of HL-60, A549, HCT-116, U2OS, CAKI-1, and HeLa. The antibody detected the target protein (~83.9 KDa) in HL-60 lysate. Click image to enlarge

CPTC-MPO-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-MPO-1 as primary antibody against the whole lysates of HL-60, A549, HCT-116, U2OS, CAKI-1, and HeLa. The antibody detected the target protein (~83.9 KDa) in HL-60 lysate.


Characterization SOP Files

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Antibody: CPTC-MPO-2
Background

Catalog Number:

CPTC-MPO-2

RRID:

AB_2814785

Target Antigen:

Myeloperoxidase Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

01/05/2026

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Flow Cytometry
Click to enlarge image Flow cytometric analysis of Myeloperoxidase (MPO) expression using CPTC-MPO-2 mouse IgG2b antibody. HL-60 cells were fixed, permeabilized, and then stained with CPTC-MPO-2 (solid green) or concentration-matched mouse IgG2b isotype control (dashed green) antibodies. HeLa cells were fixed, permeabilized, and then stained with CPTC-MPO-2 (solid blue) or concentration-matched mouse IgG2b isotype control (dashed blue) antibodies. A BV421 conjugated goat anti-mouse IgG was used as a secondary antibody. All data were analyzed using FlowJo. Click image to enlarge

CPTC-MPO-2 Flow Cytometry

Result: Uncertain

Flow cytometric analysis of Myeloperoxidase (MPO) expression using CPTC-MPO-2 mouse IgG2b antibody. HL-60 cells were fixed, permeabilized, and then stained with CPTC-MPO-2 (solid green) or concentration-matched mouse IgG2b isotype control (dashed green) antibodies. HeLa cells were fixed, permeabilized, and then stained with CPTC-MPO-2 (solid blue) or concentration-matched mouse IgG2b isotype control (dashed blue) antibodies. A BV421 conjugated goat anti-mouse IgG was used as a secondary antibody. All data were analyzed using FlowJo.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (610.5 KB)

CPTC-MPO-2 IHC evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-MPO-2 . Titer: 1:1000 Click image to enlarge

CPTC-MPO-2 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-MPO-2 . Titer: 1:1000


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining using CPTC-MPO-2 as primary antibody (pink-violet) against HeLa and HL-60 cells. HL-60 cells show MPO protein subcellular localization in the lysosome. Click image to enlarge

CPTC-MPO-2 Immunofluorescence

Result: Positive

Immunofluorescence staining using CPTC-MPO-2 as primary antibody (pink-violet) against HeLa and HL-60 cells. HL-60 cells show MPO protein subcellular localization in the lysosome.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (610.5 KB)

CPTC-MPO-2 IF evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MPO-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MPO-2 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-MPO-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot of CPTC-MPO-2 against the over-expressed lysate of MPO. Expected MW is 83.9 KDa. The antibody detected the target protein. Click image to enlarge

CPTC-MPO-2 Western Blot (Overexpressed Cell Lysate)

Result: Positive

Western Blot of CPTC-MPO-2 against the over-expressed lysate of MPO. Expected MW is 83.9 KDa. The antibody detected the target protein.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-MPO-2 as primary antibody against human myeloperoxidase (MPO), nuclear gene encoding mitochondrial recombinant protein (lane 2). Expected molecular weight - 83.7 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MPO-2 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-MPO-2 as primary antibody against human myeloperoxidase (MPO), nuclear gene encoding mitochondrial recombinant protein (lane 2). Expected molecular weight - 83.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. All other cell lines are negative. Click image to enlarge

CPTC-MPO-2 Simple Western Blot (Cell Lysate)

Result: Positive

Automated western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MPO-2 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MPO-2 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 83.9 kDa, 73.9 kDa, and 87.2 kDa. Lung, spleen, and endometrium are presumed positive (MPO heavy chain). Breast and ovary are negative. Vinculin was used as a loading control. The immunoblot was conducted under reducing conditions. Click image to enlarge

CPTC-MPO-2 Western Blot (Tissue Lysates)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-MPO-2 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 83.9 kDa, 73.9 kDa, and 87.2 kDa. Lung, spleen, and endometrium are presumed positive (MPO heavy chain). Breast and ovary are negative. Vinculin was used as a loading control. The immunoblot was conducted under reducing conditions.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-MPO-2 as a primary antibody against HeLa and HL-60 cell lysates. Relative expression of total MPO (A). Percentage of cells that express MPO (B). Average expression of MPO protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-MPO-2 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-MPO-2 as a primary antibody against HeLa and HL-60 cell lysates. Relative expression of total MPO (A). Percentage of cells that express MPO (B). Average expression of MPO protein per cell (C). All data is normalized to β-tubulin expression.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MPO-2 as primary antibody against the whole lysates of HL-60, A549, HCT-116, U2OS, CAKI-1, and HeLa. The antibody detected the target protein (~83.9 KDa) in HL-60 lysate. Click image to enlarge

CPTC-MPO-2 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-MPO-2 as primary antibody against the whole lysates of HL-60, A549, HCT-116, U2OS, CAKI-1, and HeLa. The antibody detected the target protein (~83.9 KDa) in HL-60 lysate.


Characterization SOP Files

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Antibody: CPTC-MPO-3
Background

Catalog Number:

CPTC-MPO-3

Target Antigen:

Myeloperoxidase Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

01/05/2026

Antigen Recognition(s):

Peptide, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  Flow Cytometry
Click to enlarge image Flow cytometric analysis of Myeloperoxidase (MPO) expression using CPTC-MPO-3 mouse IgG1 antibody. HL-60 cells were fixed, permeabilized, and then stained with CPTC-MPO-3 (solid green) or concentration-matched mouse IgG1 isotype control (dashed green) antibodies. HeLa cells were fixed, permeabilized, and then stained with CPTC-MPO-3 (solid blue) or concentration-matched mouse IgG1 isotype control (dashed blue) antibodies. A BV421 conjugated goat anti-mouse IgG was used as a secondary antibody. All data were analyzed using FlowJo Click image to enlarge

CPTC-MPO-3 Flow Cytometry

Result: Uncertain

Flow cytometric analysis of Myeloperoxidase (MPO) expression using CPTC-MPO-3 mouse IgG1 antibody. HL-60 cells were fixed, permeabilized, and then stained with CPTC-MPO-3 (solid green) or concentration-matched mouse IgG1 isotype control (dashed green) antibodies. HeLa cells were fixed, permeabilized, and then stained with CPTC-MPO-3 (solid blue) or concentration-matched mouse IgG1 isotype control (dashed blue) antibodies. A BV421 conjugated goat anti-mouse IgG was used as a secondary antibody. All data were analyzed using FlowJo


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (493.4 KB)

CPTC-MPO-3 IHC evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining using CPTC-MPO-3 as primary antibody (pink-violet) against HeLa and HL-60 cells. HL-60 cells show MPO protein subcellular localization in the lysosome. Click image to enlarge

CPTC-MPO-3 Immunofluorescence

Result: Positive

Immunofluorescence staining using CPTC-MPO-3 as primary antibody (pink-violet) against HeLa and HL-60 cells. HL-60 cells show MPO protein subcellular localization in the lysosome.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (493.4 KB)

CPTC-MPO-3 IF evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MPO-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MPO-3 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-MPO-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot of CPTC-MPO-3 against the over-expressed lysate of MPO. Expected MW is 83.9 KDa. The antibody detected the target protein. Click image to enlarge

CPTC-MPO-3 Western Blot (Overexpressed Cell Lysate)

Result: Positive

Western Blot of CPTC-MPO-3 against the over-expressed lysate of MPO. Expected MW is 83.9 KDa. The antibody detected the target protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-MPO-3 as a primary antibody against HeLa and HL-60 cell lysates. Relative expression of total MPO (A). Percentage of cells that express MPO (B). Average expression of MPO protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-MPO-3 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-MPO-3 as a primary antibody against HeLa and HL-60 cell lysates. Relative expression of total MPO (A). Percentage of cells that express MPO (B). Average expression of MPO protein per cell (C). All data is normalized to β-tubulin expression.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MPO-3 as primary antibody against the whole lysates of HL-60, A549, HCT-116, U2OS, CAKI-1, and HeLa. The antibody detected the target protein (~83.9 KDa) in HL-60 lysate. Click image to enlarge

CPTC-MPO-3 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-MPO-3 as primary antibody against the whole lysates of HL-60, A549, HCT-116, U2OS, CAKI-1, and HeLa. The antibody detected the target protein (~83.9 KDa) in HL-60 lysate.


Characterization SOP Files

[top]

Antigen: Myeloperoxidase Peptide 1
Background

NCI Identification Number:

00478

Antigen Name:

Myeloperoxidase Peptide 1

CPTC Name:

CPTC-MPO Peptide 1

Aliases:

Myeloperoxidase; EC 1.11.1.7; EC 1.11.2.2; EC 1.11.1; MPO

Function:

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.MPO (Myeloperoxidase) is a Protein Coding gene. Diseases associated with MPO include Myeloperoxidase Deficiency and Alzheimer Disease. Among its related pathways are Transcriptional misregulation in cancer and Folate Metabolism. Gene Ontology (GO) annotations related to this gene include chromatin binding and heme binding. An important paralog of this gene is EPX.Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Chromosomal Localization:

17q22

Accession Number:

NP_000241.1

UniProt Accession Number:

P05164

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

IGLDLPALNMQR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

12/17/2020

Links

Characterization Data

SOPs

No SOPs available.

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