PRINT

Myeloperoxidase Peptide 1

Antibody: CPTC-MPO-1
Background

Catalog Number:

CPTC-MPO-1

RRID:

AB_2814784

Target Antigen:

Myeloperoxidase Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

02/15/2024

Antigen Recognition(s):

Peptide

Purchase
External Links
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (505.4 KB)

CPTC-MPO-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (505.4 KB)

CPTC-MPO-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

[top]

Antibody: CPTC-MPO-2
Background

Catalog Number:

CPTC-MPO-2

RRID:

AB_2814785

Target Antigen:

Myeloperoxidase Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

02/15/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (610.5 KB)

CPTC-MPO-2 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-MPO-2 . Titer: 1:1000 Click image to enlarge

CPTC-MPO-2 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-MPO-2 . Titer: 1:1000


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (610.5 KB)

CPTC-MPO-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MPO-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-MPO-2 as primary antibody against human myeloperoxidase (MPO), nuclear gene encoding mitochondrial recombinant protein (lane 2). Expected molecular weight - 83.7 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MPO-2 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-MPO-2 as primary antibody against human myeloperoxidase (MPO), nuclear gene encoding mitochondrial recombinant protein (lane 2). Expected molecular weight - 83.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. All other cell lines are negative. Click image to enlarge

CPTC-MPO-2 Automated Western Blot Cell Lysate

Result: Positive

Automated western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MPO-2 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MPO-2 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 83.9 kDa, 73.9 kDa, and 87.2 kDa. Lung, spleen, and endometrium are presumed positive (MPO heavy chain). Breast and ovary are negative. Vinculin was used as a loading control. The immunoblot was conducted under reducing conditions. Click image to enlarge

CPTC-MPO-2 Western Blot (Tissue Lysates)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-MPO-2 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 83.9 kDa, 73.9 kDa, and 87.2 kDa. Lung, spleen, and endometrium are presumed positive (MPO heavy chain). Breast and ovary are negative. Vinculin was used as a loading control. The immunoblot was conducted under reducing conditions.


Characterization SOP Files

[top]

Antibody: CPTC-MPO-3
Background

Catalog Number:

CPTC-MPO-3

Target Antigen:

Myeloperoxidase Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

10/18/2023

Antigen Recognition(s):

Peptide

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (493.4 KB)

CPTC-MPO-3 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (493.4 KB)

CPTC-MPO-3 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

[top]

Antigen: Myeloperoxidase Peptide 1
Background

NCI Identification Number:

00478

Antigen Name:

Myeloperoxidase Peptide 1

CPTC Name:

CPTC-MPO Peptide 1

Aliases:

Myeloperoxidase; EC 1.11.1.7; EC 1.11.2.2; EC 1.11.1; MPO

Function:

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.MPO (Myeloperoxidase) is a Protein Coding gene. Diseases associated with MPO include Myeloperoxidase Deficiency and Alzheimer Disease. Among its related pathways are Transcriptional misregulation in cancer and Folate Metabolism. Gene Ontology (GO) annotations related to this gene include chromatin binding and heme binding. An important paralog of this gene is EPX.Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Chromosomal Localization:

17q22

Accession Number:

NP_000241.1

UniProt Accession Number:

P05164

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

IGLDLPALNMQR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

12/17/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]