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Methyl CpG Binding Protein 1

Antibody: CPTC-MBD1-1
Background

Catalog Number:

CPTC-MBD1-1

RRID:

AB_1553730

Target Antigen:

Methyl CpG Binding Protein 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

05/28/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on ovarian cancer tissue core using CPTC-MBD1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, placenta, prostate, pancreas, breast, lung, endometrium, appendix, and spleen) and cancer tissues (breast, prostate, colon, ovarian, and lung). Click image to enlarge

CPTC-MBD1-1 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on ovarian cancer tissue core using CPTC-MBD1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, placenta, prostate, pancreas, breast, lung, endometrium, appendix, and spleen) and cancer tissues (breast, prostate, colon, ovarian, and lung).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (180.1 KB)

CPTC-MBD1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-MBD1-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-MBD1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-MBD1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MBD1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MBD1-1 as primary Ab against Ag 10436 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MBD1-1 Western Blot

Result: Positive

Western Blot using CPTC-MBD1-1 as primary Ab against Ag 10436 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-MBD1-2
Background

Catalog Number:

CPTC-MBD1-2

RRID:

AB_1553732

Target Antigen:

Methyl CpG Binding Protein 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2021

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (256.3 KB)

CPTC-MBD1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-MBD1-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-MBD1-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-MBD1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MBD1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MBD1-2 as primary Ab against Ag 10436 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MBD1-2 Western Blot

Result: Positive

Western Blot using CPTC-MBD1-2 as primary Ab against Ag 10436 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-MBD1-3
Background

Catalog Number:

CPTC-MBD1-3

RRID:

AB_2089800

Target Antigen:

Methyl CpG Binding Protein 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2021

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (256.3 KB)

CPTC-MBD1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-MBD1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MBD1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MBD1-3 Protein Array

Result: Positive

Protein Array in which CPTC-MBD1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MBD1-3 as primary Ab against Ag 10436 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MBD1-3 Western Blot

Result: Positive

Western Blot using CPTC-MBD1-3 as primary Ab against Ag 10436 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Methyl CpG Binding Protein 1
Background

NCI Identification Number:

10436

Antigen Name:

Methyl CpG Binding Protein 1

CPTC Name:

CPTC-MBD1

Aliases:

MBD1; CXXC3; OTTHUMP00000163504; OTTHUMP00000163506; OTTHUMP00000163507; PCM1; RFT

Function:

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in
mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of
these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five
transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from
methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor
and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as
the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1
and isoform 2 can also repress transcription from unmethylated promoters.

Chromosomal Localization:

18q21

Expression System:

E. Coli

Accession Number:

BC000453

UniProt Accession Number:

Q9UIS9

DNA Source:

HIP:HsCD00022173

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

17023

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEK
NKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQ
AELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQ
INTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGE
PAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLK
EQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEAS
DTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQH
KAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQP
PAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQ
KMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGES
NSLTSSVPYPTASLVSQNESENEGHLNPSEKLQ

Native Sequence:

MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKK
KFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAEL
EKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINT
NKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAM
ESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQE
KSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTT
ARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAE
QAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAV
PDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMD
KLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSL
TSSVPYPTASLVSQNESENEGHLNPSEKLQ

Calculated Isoelectric Point:

5.61

Molecular Weight:

59471

Last Updated:

09/01/2008

Links

Characterization Data

Gel

Click to enlarge image SDS-PAGE of Ag 10295 with molecular weight standards
Click image to enlarge

Ag 10436

SDS-PAGE of Ag 10295 with molecular weight standards

No SOPs available.

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SOPs

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