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Lymphocyte Antigen 6 Family Member K

Antibody: CPTC-Ly6K-1
Background

Catalog Number:

CPTC-Ly6K-1

Target Antigen:

Lymphocyte Antigen 6 Family Member K

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Recombinant Full-length

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR

CPTC-LY6K-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-LY6K-1 and full length LY6K recombinant protein were measured using surface plasmon resonance. LY6K recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6K-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-Ly6K-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

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Antibody: CPTC-Ly6K-2
Background

Catalog Number:

CPTC-Ly6K-2

Target Antigen:

Lymphocyte Antigen 6 Family Member K

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR

CPTC-LY6K-2 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-LY6K-2 and full length LY6K recombinant protein were measured using surface plasmon resonance. LY6K recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6K-2 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-Ly6K-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

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Antibody: CPTC-Ly6K-3
Background

Catalog Number:

CPTC-Ly6K-3

Target Antigen:

Lymphocyte Antigen 6 Family Member K

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Recombinant Full-length

Purchase
Publications
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-LY6K-3 and full length LY6K recombinant protein were measured using surface plasmon resonance. LY6K recombinant protein was amine coupled onto a Series S CM5 biosensor chip. CPTC-LY6K-3 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a 1:2 bivalent fitting model. Click image to enlarge

CPTC-LY6K-3 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Positive

Affinity and binding kinetics of CPTC-LY6K-3 and full length LY6K recombinant protein were measured using surface plasmon resonance. LY6K recombinant protein was amine coupled onto a Series S CM5 biosensor chip. CPTC-LY6K-3 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (299.9 KB)

CPTC-Ly6K-3 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-LY6K-3 as primary mouse antibody against full length LY6K recombinant protein. Recombinant protein was coated on the plate and CPTC-LY6K-3 antibody was titrated and detected using the goat anti-mouse antibody and TMB. Click image to enlarge

CPTC-LY6K-3 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-LY6K-3 as primary mouse antibody against full length LY6K recombinant protein. Recombinant protein was coated on the plate and CPTC-LY6K-3 antibody was titrated and detected using the goat anti-mouse antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-Ly6K-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-Ly6K-3 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-Ly6K-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

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Antigen: Lymphocyte Antigen 6 Family Member K
Background

NCI Identification Number:

00235

Antigen Name:

Lymphocyte Antigen 6 Family Member K

CPTC Name:

CPTC-Ly6K

Aliases:

Lymphocyte Antigen 6 Family Member K; Lymphocyte Antigen 6 Complex, Locus K; Cancer/Testis Antigen 97; Ly-6K; Up-Regulated In Lung Cancer 10; Lymphocyte Antigen 6K; HSJ001348; URLC10; CT97; CO16; FLJ35226

Function:

Predicted to be involved in binding activity of sperm to zona pellucida. Predicted to act upstream of or within flagellated sperm motility. Predicted to be located in cell surface; cytoplasm; and plasma membrane. Predicted to be active in acrosomal vesicle. [provided by Alliance of Genome Resources, Apr 2022]LY6K (Lymphocyte Antigen 6 Family Member K) is a Protein Coding gene. Diseases associated with LY6K include Lung Cancer. Among its related pathways are Metabolism of proteins and Post-translational modification: synthesis of GPI-anchored proteins. An important paralog of this gene is GML.Required for sperm migration into the oviduct and male fertility by controlling binding of sperm to zona pellucida (By similarity). May play a role in cell growth.

Chromosomal Localization:

8q24.3

Expression System:

E.coli

Accession Number:

NP_001153826.1

UniProt Accession Number:

Q17RY6

DNA Source:

N/A

Immunogen:

Recombinant Full Length Protein

Vector Name:

pET 28 HA tag

Extinction Coefficient:

Buffers:

0.5% Triton buffer

Expressed Sequence:

MRLQRPRQAPAGGRRAPRGGRGSPYRPDPGRGARRLRRFQKGGEGAPRAD
PPWAPLGTMALLALLLVVALPRVWTDANLTARQRDPEDSQRTDEGDNRVW
CHVCERENTFECQNPRRCKWTEPYCVIAAVKIFPRFFMVAKQCSAGCAAM
ERPKPEEKRFLLEEPMPFFYLKCCKIRYCNLEGPPINSSVFKEYAGSMGE
SCGGLWLAIL LLLASIAAGLSLS

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

15400

Last Updated:

03/06/2023

Links

Characterization Data

SOPs

No SOPs available.

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