Catalog Number:
CPTC-Ly6K-1
Target Antigen:
Lymphocyte Antigen 6 Family Member K
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Recombinant Full-length
Result: Negative
Affinity and binding kinetics of CPTC-LY6K-1 and full length LY6K recombinant protein were measured using surface plasmon resonance. LY6K recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6K-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Catalog Number:
CPTC-Ly6K-2
Target Antigen:
Lymphocyte Antigen 6 Family Member K
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Recombinant Full-length
Result: Negative
Affinity and binding kinetics of CPTC-LY6K-2 and full length LY6K recombinant protein were measured using surface plasmon resonance. LY6K recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6K-2 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Catalog Number:
CPTC-Ly6K-3
Target Antigen:
Lymphocyte Antigen 6 Family Member K
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Recombinant Full-length
Result: Positive
Affinity and binding kinetics of CPTC-LY6K-3 and full length LY6K recombinant protein were measured using surface plasmon resonance. LY6K recombinant protein was amine coupled onto a Series S CM5 biosensor chip. CPTC-LY6K-3 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a 1:2 bivalent fitting model.
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA using CPTC-LY6K-3 as primary mouse antibody against full length LY6K recombinant protein. Recombinant protein was coated on the plate and CPTC-LY6K-3 antibody was titrated and detected using the goat anti-mouse antibody and TMB.
Result: Positive
Protein Array in which CPTC-Ly6K-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
NCI Identification Number:
00235
Antigen Name:
Lymphocyte Antigen 6 Family Member K
CPTC Name:
CPTC-Ly6K
Aliases:
Lymphocyte Antigen 6 Family Member K; Lymphocyte Antigen 6 Complex, Locus K; Cancer/Testis Antigen 97; Ly-6K; Up-Regulated In Lung Cancer 10; Lymphocyte Antigen 6K; HSJ001348; URLC10; CT97; CO16; FLJ35226
Function:
Predicted to be involved in binding activity of sperm to zona pellucida. Predicted to act upstream of or within flagellated sperm motility. Predicted to be located in cell surface; cytoplasm; and plasma membrane. Predicted to be active in acrosomal vesicle. [provided by Alliance of Genome Resources, Apr 2022]LY6K (Lymphocyte Antigen 6 Family Member K) is a Protein Coding gene. Diseases associated with LY6K include Lung Cancer. Among its related pathways are Metabolism of proteins and Post-translational modification: synthesis of GPI-anchored proteins. An important paralog of this gene is GML.Required for sperm migration into the oviduct and male fertility by controlling binding of sperm to zona pellucida (By similarity). May play a role in cell growth.
Chromosomal Localization:
8q24.3
Expression System:
E.coli
Accession Number:
NP_001153826.1
UniProt Accession Number:
Q17RY6
DNA Source:
N/A
Immunogen:
Recombinant Full Length Protein
Vector Name:
pET 28 HA tag
Extinction Coefficient:
Buffers:
0.5% Triton buffer
Expressed Sequence:
MRLQRPRQAPAGGRRAPRGGRGSPYRPDPGRGARRLRRFQKGGEGAPRAD
PPWAPLGTMALLALLLVVALPRVWTDANLTARQRDPEDSQRTDEGDNRVW
CHVCERENTFECQNPRRCKWTEPYCVIAAVKIFPRFFMVAKQCSAGCAAM
ERPKPEEKRFLLEEPMPFFYLKCCKIRYCNLEGPPINSSVFKEYAGSMGE
SCGGLWLAIL LLLASIAAGLSLS
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
15400
Last Updated:
03/06/2023
No SOPs available.
Get it for free at Adobe.com