Background
Catalog Number:
CPTC-LIG4-1
Target Antigen:
DNA Ligase 4 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
07/19/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-LIG4-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (540.3 KB)
CPTC-LIG4-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (540.3 KB)
CPTC-LIG4-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Background
NCI Identification Number:
00441
Antigen Name:
DNA Ligase 4 Peptide 1
CPTC Name:
CPTC-LIG4 Peptide 1
Aliases:
DNA Ligase 4; Polydeoxyribonucleotide Synthase [ATP] 4; Ligase IV, DNA, ATP-Dependent; Polynucleotide Ligase; DNA Repair Enzyme; DNA Ligase IV; DNA Joinase; EC 6.5.1.1; Sealase; LIG4S; LIG4
Function:
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.LIG4 (DNA Ligase 4) is a Protein Coding gene. Diseases associated with LIG4 include Lig4 Syndrome and Myeloma, Multiple. Among its related pathways are DNA Double Strand Break Response and Chks in Checkpoint Regulation. Gene Ontology (GO) annotations related to this gene include ligase activity and DNA ligase activity. An important paralog of this gene is LIG3.Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. Ligases are enzymes which catalyze the joining of two molecules with concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate. They belong to the E.C. 6 class of enzymes, which also includes synthases and carboxylases.
Chromosomal Localization:
13q33.3
Accession Number:
NP_001091738.1
UniProt Accession Number:
P49917
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LILPQLER
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
880
Last Updated:
09/30/2020
Links
- EnsEMBL - EnsEMBL LIG4
- Entrez - Entrez LIG4
- Uniprot - Uniprot P49917
Characterization Data
SOPs
No SOPs available.
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