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DNA Ligase 1 Peptide 1

Antibody: CPTC-LIG1-1
Background

Catalog Number:

CPTC-LIG1-1

Target Antigen:

DNA Ligase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC Tissue

CPTC-LIG1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IP

CPTC-LIG1-1 Immunoprecipitation

Result: Negative

Immuno-precipitation performed using CPTC-LIG1-1 as capture antibody against the over-expressed lysate of LIG1 (Myc-tagged). Eluates were tested in automated western blot using the an anti-Myc tag as detection antibody. The target protein was not pulled down.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-LIG1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6217) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-LIG1-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-LIG1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6217)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CPTC-LIG1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-LIG1-1 as primary antibody against human ligase I, DNA, ATP-dependent (LIG1) recombinant protein (lane 2). Expected molecular weight - 101.6 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-LIG1-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-LIG1-1 as primary antibody against human ligase I, DNA, ATP-dependent (LIG1) recombinant protein (lane 2). Expected molecular weight - 101.6 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-LIG1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 101.7 kDa, 88.5 kDa, and 98.2 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-LIG1-1 Western Blot Cell Lysate

Result: Positive

Western blot using CPTC-LIG1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 101.7 kDa, 88.5 kDa, and 98.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-LIG1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 101.7 kDa, 88.5 kDa, and 98.2 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-LIG1-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-LIG1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 101.7 kDa, 88.5 kDa, and 98.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-LIG1-1 Western Blot (Tissue Lysates)

Result: Negative

Western blot using CPTC-LIG1-1 as primary antibody against human breast (2), ovary (3), spleen (4), endometrium (5), and lung (6) tissue lysates. The expected molecular weight is 101.7 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

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Antigen: DNA Ligase 1 Peptide 1
Background

NCI Identification Number:

00377

Antigen Name:

DNA Ligase 1 Peptide 1

CPTC Name:

CPTC-LIG1 Peptide 1

Aliases:

DNA Ligase 1; Polydeoxyribonucleotide Synthase [ATP] 1; Ligase I, DNA, ATP-Dependent; EC 6.5.1.1; DNA Ligase I; LIG1

Function:

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.LIG1 (DNA Ligase 1) is a Protein Coding gene. Diseases associated with LIG1 include Bloom Syndrome and Xeroderma Pigmentosum, Variant Type. Among its related pathways are Transcription-Coupled Nucleotide Excision Repair (TC-NER) and Telomere C-strand (Lagging Strand) Synthesis. Gene Ontology (GO) annotations related to this gene include DNA ligase activity and DNA ligase (ATP) activity. An important paralog of this gene is LIG3.DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Ligases are enzymes which catalyze the joining of two molecules with concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate. They belong to the E.C. 6 class of enzymes, which also includes synthases and carboxylases.

Chromosomal Localization:

19q13.33

Accession Number:

NP_000225.1

UniProt Accession Number:

P18858

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

AQIHALEGGEVK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

09/02/2020

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Characterization Data

SOPs

No SOPs available.

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