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La Ribonucleoprotein 6, Translational Regulator Peptide 1

Antibody: CPTC-LARP6-1
Background

Catalog Number:

CPTC-LARP6-1

Target Antigen:

La Ribonucleoprotein 6, Translational Regulator Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

05/15/2025

Antigen Recognition(s):

Peptide, Endogenous

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-LARP6-1 and BSA conjugated peptide, CALA-pT-PQKNGRV, were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled to the AR2G biosensors. The CPTC-LARP6-1 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data were analyzed globally using a 1:2 (bivalent) fitting model. Click image to enlarge

CPTC-LARP6-1 Affinity and Kinetics (Bio-Layer Interferometry)

Result: High Binding

Affinity and binding kinetics of CPTC-LARP6-1 and BSA conjugated peptide, CALA-pT-PQKNGRV, were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled to the AR2G biosensors. The CPTC-LARP6-1 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data were analyzed globally using a 1:2 (bivalent) fitting model.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-LARP6-1 and BSA-conjugated peptide, CALA-pT-PQKNGRV, were measured using surface plasmon resonance. BSA-conjugated peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-LARP6-1 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte. All data were double referenced and analyzed globally using a 1:2 (bivalent) fitting model. Click image to enlarge

CPTC-LARP6-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: High Binding

Affinity and binding kinetics of CPTC-LARP6-1 and BSA-conjugated peptide, CALA-pT-PQKNGRV, were measured using surface plasmon resonance. BSA-conjugated peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-LARP6-1 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte. All data were double referenced and analyzed globally using a 1:2 (bivalent) fitting model.


Characterization SOP Files

  CyTOF
Click to enlarge image Imaging mass cytometry on colon cancer tissue core using CPTC-LARP61-1 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (endometrium, spleen, placenta, prostate, colon, pancreas, breast, lung, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, lung, and prostate). Click image to enlarge

CPTC-LARP6-1 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on colon cancer tissue core using CPTC-LARP61-1 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (endometrium, spleen, placenta, prostate, colon, pancreas, breast, lung, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, lung, and prostate).


Characterization SOP Files

  Flow Cytometry

CPTC-LARP6-1 (Flow Cytometry)

Result: Negative

Flow cytometric analysis of thyroid medullary TT cells, asynchronous (blue) or synchronous (serum starved 24 hours, green) using CPTC-LARP6-1 rabbit antibody (solid lines) or concentration matched rabbit isotype control antibody (dashed lines). TT cells were permeabilized, fixed, and then stained with either CPTC-LARP6-1 or rabbit isotype control antibody. A BV421 conjugated goat anti-rabbit IgG (H+L) was used as a secondary antibody. All data were analyzed using FlowJo.


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast cancer showing nuclear staining using Antibody CPTC-LARP6-1. Titer: 1:6250 Click image to enlarge

CPTC-LARP6-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of breast cancer showing nuclear staining using Antibody CPTC-LARP6-1. Titer: 1:6250


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-LARP6-1 as primary antibody against BSA-conjugated peptide, CALA-pT-PQKNGRV, coated on the plate and detected using the goat anti- rabbit antibody and TMB. Click image to enlarge

CPTC-LARP6-1 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-LARP6-1 as primary antibody against BSA-conjugated peptide, CALA-pT-PQKNGRV, coated on the plate and detected using the goat anti- rabbit antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-LARP6-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-LARP6-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-LARP6-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate

CPTC-LARP6-1 WB (Over-expressed lysates)

Result: Negative

Western Blot using CPTC-LARP6-1 as primary antibody against the over-expressed lysate of LARP6. The antibody is not able to recognize the target protein in the tested lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-LARP6-1 WB (Cell lysates)

Result: Negative

Western blot using CPTC-LARP6-1 as primary antibody against the cell lysates of TT, H146 and H1184 cells, asynchronous (+) or synchronous - serum starved 24hours (-). The antibody was not able to detect the target protein in any of the tested cell lysates. The same membrane was probed with an anti CytC antibody, and all the cell lysates show the presence of the housekeeping protein.


Characterization SOP Files

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Antigen: La Ribonucleoprotein 6, Translational Regulator Peptide 1
Background

NCI Identification Number:

00537

Antigen Name:

La Ribonucleoprotein 6, Translational Regulator Peptide 1

CPTC Name:

CPTC-LARP6 Peptide 1

Aliases:

La Ribonucleoprotein 6, Translational Regulator; Acheron; La Ribonucleoprotein Domain Family Member 6; La-Related Protein 6; FLJ11196; La Ribonucleoprotein Domain Family, Member 6; Death-Associated LA Motif Protein; ACHN; Achn

Function:

Enables RNA binding activity and myosin binding activity. Involved in positive regulation of collagen biosynthetic process; positive regulation of mRNA binding activity; and positive regulation of translation. Located in nucleus. Part of polysome.LARP6 (La Ribonucleoprotein 6, Translational Regulator) is a Protein Coding gene. Diseases associated with LARP6 include Ovarian Endometrial Cancer and Retrograde Amnesia. Gene Ontology (GO) annotations related to this gene include RNA binding and nucleotide binding. An important paralog of this gene is LARP1.Regulates the coordinated translation of type I collagen alpha-1 and alpha-2 mRNAs, CO1A1 and CO1A2. Stabilizes mRNAs through high-affinity binding of a stem-loop structure in their 5' UTR. This regulation requires VIM and MYH10 filaments, and the helicase DHX9.

Chromosomal Localization:


15q23

Accession Number:

UniProt Accession Number:

Q9BRS8 Previous GeneCards

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CALA-pT-PQKNGRV

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

11/18/2022

Links

Characterization Data

SOPs

No SOPs available.

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