Background
Catalog Number:
CPTC-KRT Group A-1
Target Antigen:
Keratin Group A Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
07/30/2021
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (341.7 KB)
CPTC-KRT Group A-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC Tissue
CTPC-KRT Group A-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Immunofluorescence
CPTC-KRT Group A-1
Result: Positive
Immunofluorescence staining of human cell line HeLa with CPTC-KRT Group A-1 Ab shows localization to the nucleus, other.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KRT Group A-1 Western Blot (Recombinat Protein)
Result: Positive
Western Blot using CPTC-KRT Group A-1 as primary antibody against KRT19 recombinant protein (lane 2). Also included are molecular weight standard (lane 1)
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-KRT Group A-1 Western Blot (Cell Lysates)
Result: Positive
Western Blot using CPTC-KRT Group A-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standard (lane 1)
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-KRT Group A-1 Single Cell Western Blot (Cell Lysate)
Result: Positive
Single cell western blot using CPTC-KRT Group A-1 as a primary antibody against cell lysates. Relative expression of total KRT Group A in HeLa and MCF10A cells (A). Percentage of cells that express KRT Group A (B). Average expression of KRT Group A protein per cell (C). All data is normalized to β-tubulin expression.
Characterization SOP Files
Background
NCI Identification Number:
00318
Antigen Name:
Keratin Group A Peptide 1
CPTC Name:
CPTC-KRT Group A Peptide 1
Aliases:
KRT24; Keratin 24; Keratin, Type I Cytoskeletal 24; Keratin 24, Type I; Type I Keratin-24; Cytokeratin-24; CK-24; KA24; K24; Keratin-24; KRT36; Keratin 36; Keratin, Type I Cuticular Ha6; Hair Keratin, Type I Ha6; Keratin, Hair, Acidic, 6; Keratin 36, Type I; KRTHA6; K36; Keratin-36; HHa6; HHA6; HKA6; HA6; KRT10; Keratin 10; Keratin, Type I Cytoskeletal 10; Keratin 10, Type I; Cytokeratin 10; CK-10; KPP; K10; Keratosis Palmaris Et Plantaris; Epidermolytic Hyperkeratosis; Cytokeratin-10; Keratin-10; BCIE; CK10; BIE; EHK; KRT13; Keratin 13; Keratin, Type I Cytoskeletal 13; Keratin 13, Type I; Cytokeratin 13; CK-13; K13; Cytokeratin-13; Keratin-13; CK13; WSN2; KRT33B; Keratin 33B; Keratin, Type I Cuticular Ha3-II; Hair Keratin, Type I Ha3-II; Keratin, Hair, Acidic, 3B; Keratin 33B, Type I; KRTHA3B; K33B; Hard Keratin, Type I, 3II; Hard Keratin Type I 3II; Type I Hair Keratin 3B; Keratin-33B; KRTHA3A; HHa3-II; HHA3-II; Ha-3II; HA3II; HKA3B; KRT31; Keratin 31; Keratin, Type I Cuticular Ha1; Hair Keratin, Type I Ha1; Keratin 31, Type I; KRTHA1; K31; Keratin, Hair, Acidic, 1; Hard Keratin, Type I, 1; Keratin, Hair, Acidic,1; Hard Keratin Type I 1; Keratin-31; Ha-1; HHa1; HHA1; HKA1; HA1; KRT16; Keratin 16; Focal Non-Epidermolytic Palmoplantar Keratoderma; Keratin, Type I Cytoskeletal 16; Keratin 16, Type I; KRT16A; K16; Cytokeratin 16; Cytokeratin-16; Keratin-16; FNEPPK; NEPPK; CK-16; CK16; K1CP; PC1; KRT15; Keratin 15; Keratin, Type I Cytoskeletal 15; Type I Cytoskeletal 15; Keratin 15, Type I; Keratin-15, Basic; Keratin-15, Beta; Cytokeratin 15; CK-15; K15; Cytokeratin-15; Keratin-15; CK15; K1CO; KRTB; KRT32; Keratin 32; Keratin, Type I Cuticular Ha2; Hair Keratin, Type I Ha2; Keratin, Hair, Acidic, 2; Keratin 32, Type I; KRTHA2; HKA2; K32; Type I Cuticular Hair Keratin; Hard Keratin, Type I, 2; Hard Keratin Type I; Keratin-32; HHa2; HHA2; HA2; KRT38; Keratin 38; Keratin, Type I Cuticular Ha8; Hair Keratin, Type I Ha8; Keratin, Hair, Acidic, 8; Keratin 38, Type I; KRTHA8; K38; Keratin-38; HHa8; HHA8; HKA8; HA8; KRT37; Keratin 37; Keratin, Type I Cuticular Ha7; Hair Keratin, Type I Ha7; Keratin, Hair, Acidic, 7; Keratin 37, Type I; KRTHA7; K37; Keratin-37; HHA7; HKA7; KRT14
Function:
A peptide homologous to a group of Keratin Group A proteins (Keratin Group A: KRT24, KRT36, KRT10, KRT13, KRT33B, KRT31, KRT16, KRT15, KRT32, KRT38, KRT37, KRT14 KRT28 KRT17 KRT19 KRT18 - JUP KRT35 KRT17;KRT13 MGC102966) was used for antibody generation. KRT24: This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. KRT36: The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. KRT10: This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. KRT13: The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. KRT33B: This gene encodes a member of the keratin gene family. This gene is one of multiple type I hair keratin genes that are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, the encoded protein is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, keratin 33A and keratin 33B. KRT31: The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. KRT16: The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. KRT15: The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. KRT32: he protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. KRT38: The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. KRT37: The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. KRT14:
Chromosomal Localization:
KRT24 - 17q21.2; KRT36 - 17q21.2; KRT10 - 17q21.2; KRT13 - 17q21.2; KRT33B - 17q21.2; KRT31 - 17q21.2; KRT16 - 17q21.2; KRT15 - 17q21.2; KRT32 - 17q21.2; KRT38 - 17q21.2: KRT37 - 17q21.2; KRT14 -
Accession Number:
N/A
UniProt Accession Number:
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
CLAADDFR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
880
Last Updated:
10/02/2020
Links
Characterization Data
SOPs
No SOPs available.
Don't have Adobe Reader™?
Get it for free at Adobe.com