
Catalog Number:
CPTC-KRAS4B-1
RRID:
AB_2722074
Target Antigen:
Kirsten Rat Sarcoma Viral Oncogene Homolog
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
10/03/2019
Antigen Recognition(s):
Recombinant Full-length
Thematic Panel(s):
Ras Pathway
SOP:
Result: Positive
Western blot of CPTC-KRAS4b-1 antibody with full length KRAS4a,KRAS4b, H-RAS and N-RAS recombinant proteins.
Result: Positive
This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-1 antibody.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Western blot of CPTC-KRAS4b-1 antibody with full length KRAS4b recombinant protein.
Catalog Number:
CPTC-KRAS4B-2
RRID:
AB_2722075
Target Antigen:
Kirsten Rat Sarcoma Viral Oncogene Homolog
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length
Thematic Panel(s):
Ras Pathway
SOP:
Result: Positive
Western blot of CPTC-KRAS4b-2 antibody with full length KRAS4a,KRAS4b, H-RAS and N-RAS recombinant proteins.
Result: Positive
This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-2 antibody.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Western blot of CPTC-KRAS4b-2 antibody with full length KRAS4b, recombinant protein.
Catalog Number:
CPTC-KRAS4B-3
RRID:
AB_2722076
Target Antigen:
Kirsten Rat Sarcoma Viral Oncogene Homolog
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length
Thematic Panel(s):
Ras Pathway
SOP:
Result: Positive
Western blot of CPTC-KRAS4b-3 antibody with full length KRAS4a,KRAS4b, H-RAS and N-RAS recombinant proteins.
Result: Positive
This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-3 antibody.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Western blot of CPTC-KRAS4b-3 antibody with full length KRAS4b recombinant protein.
Result: Positive
Western blot of CPTC-KRAS4b-3 antibody with full length KRAS4b recombinant protein.
Catalog Number:
CPTC-KRAS4B-4
RRID:
AB_2722077
Target Antigen:
Kirsten Rat Sarcoma Viral Oncogene Homolog
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length
Thematic Panel(s):
Ras Pathway
SOP:
Result: Positive
Western blot of CPTC-KRAS4b-4 antibody with full length KRAS4a,KRAS4b, H-RAS and N-RAS recombinant proteins.
Result: Positive
This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-4 antibody. (Note that this antibody does not pull down KRAS4A antigen.)
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Western blot of CPTC-KRAS4b-3 antibody with full length KRAS4b recombinant protein.
NCI Identification Number:
00125
Antigen Name:
Kirsten Rat Sarcoma Viral Oncogene Homolog
CPTC Name:
CPTC-KRAS4B
Keywords:
Kirsten Rat Sarcoma Viral Oncogene Homolog; KRAS2; RASK2; V-Ki-Ras2 Kirsten Rat Sarcoma 2 Viral Oncogene Homolog; C-Ki-Ras; K-Ras 2; C-K-RAS; KI-RAS; CFC2; NS; V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog; Cellular C-Ki-Ras2 Proto-Oncogene; Transforming Protein P21; PR310 C-K-Ras Oncogene; C-Kirsten-Ras Protein; K-Ras P21 Protein; Oncogene KRAS2; GTPase KRas; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; NS3
Function:
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
KRAS (Kirsten Rat Sarcoma Viral Oncogene Homolog) is a Protein Coding gene. Diseases associated with KRAS include noonan syndrome 3 and cardiofaciocutaneous syndrome 2. Among its related pathways are PI3K-Akt signaling pathway and PI-3K cascade. GO annotations related to this gene include GTP binding and GDP binding. An important paralog of this gene is HRAS.
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation.
Chromosomal Localization:
12p12.1
Expression System:
E. coli
NCBI Accession Number:
NP_004976.2
UniProt Accession Number:
P01116
DNA Source:
R999-X117-566
Immunogen:
Recombinant Full Length Protein
Vector Name:
pDest-566
Extinction Coefficient:
Buffers:
Expressed Sequence:
GGSGTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVID
GETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYR
EQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAK
TRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
21552
Last Updated:
11/17/2015
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