Background
Catalog Number:
CPTC-KRAS4B-1
RRID:
AB_2722074
Target Antigen:
KRAS Proto-Oncogene, GTPase
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
10/20/2023
Antigen Recognition(s):
Recombinant Full-length
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-KRAS4B-1
Publications
Characterization Data [Compare Characterization Data]
Cross Reactivity Data
CPTC-KRAS4B-1 Cross Reactivity
Result: Positive
Western blot of CPTC-KRAS4b-1 antibody with full length KRAS4a,KRAS4b, H-RAS and N-RAS recombinant proteins.
Characterization SOP Files
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (460.8 KB)
CPTC-KRAS4B-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IP
Download This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-1 antibody. (1.4 MB)
CPTC-KRAS4B-1 IPMS
Result: Positive
This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-1 antibody.
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (460.8 KB)
CPTC-KRAS4B-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Indirect ELISA
CPTC-KRAS4B-1 Indirect ELISA
Result: High Binding
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KRAS4B-1 Western blot
Result: Positive
Western blot of CPTC-KRAS4b-1 antibody with full length KRAS4b recombinant protein.
Characterization SOP Files
Background
Catalog Number:
CPTC-KRAS4B-2
RRID:
AB_2722075
Target Antigen:
KRAS Proto-Oncogene, GTPase
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
10/20/2023
Antigen Recognition(s):
Recombinant Full-length
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-KRAS4B-2
Publications
Characterization Data [Compare Characterization Data]
Cross Reactivity Data
CPTC-KRAS4B-2 Cross Reactivity
Result: Positive
Western blot of CPTC-KRAS4b-2 antibody with full length KRAS4a,KRAS4b, H-RAS and N-RAS recombinant proteins.
Characterization SOP Files
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (548.5 KB)
CPTC-KRAS4B-2 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IP
Download This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-2 antibody. (1.4 MB)
CPTC-KRAS4B-2 IPMS
Result: Positive
This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-2 antibody.
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (548.5 KB)
CPTC-KRAS4B-2 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Indirect ELISA
CPTC-KRAS4B-2 Indirect ELISA
Result: High Binding
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KRAS4B-2 Western blot
Result: Positive
Western blot of CPTC-KRAS4b-2 antibody with full length KRAS4b, recombinant protein.
Characterization SOP Files
Background
Catalog Number:
CPTC-KRAS4B-3
RRID:
AB_2722076
Target Antigen:
KRAS Proto-Oncogene, GTPase
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
10/20/2023
Antigen Recognition(s):
Recombinant Full-length
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-KRAS4B-3
Publications
Characterization Data [Compare Characterization Data]
Cross Reactivity Data
CPTC-KRAS4B-3 Cross Reactivity
Result: Positive
Western blot of CPTC-KRAS4b-3 antibody with full length KRAS4a,KRAS4b, H-RAS and N-RAS recombinant proteins.
Characterization SOP Files
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (447.2 KB)
CPTC-KRAS4B-3 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IP
Download This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-3 antibody. (1.3 MB)
CPTC-KRAS4B-3 IPMS
Result: Positive
This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-3 antibody.
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (447.2 KB)
CPTC-KRAS4B-3 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Indirect ELISA
CPTC-KRAS4B-3 Indirect ELISA
Result: High Binding
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KRAS4B-3 Western blot
Result: Positive
Western blot of CPTC-KRAS4b-3 antibody with full length KRAS4b recombinant protein.
Characterization SOP Files
Background
Catalog Number:
CPTC-KRAS4B-4
RRID:
AB_2722077
Target Antigen:
KRAS Proto-Oncogene, GTPase
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
10/20/2023
Antigen Recognition(s):
Recombinant Full-length
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-KRAS4B-4
Publications
Characterization Data [Compare Characterization Data]
Cross Reactivity Data
CPTC-KRAS4B-4 Cross Reactivity
Result: Positive
Western blot of CPTC-KRAS4b-4 antibody with full length KRAS4a,KRAS4b, H-RAS and N-RAS recombinant proteins.
Characterization SOP Files
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (473.4 KB)
CPTC-KRAS4B-4 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IP
Download This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-4 antibody. (Note that this antibody does not pull down KRAS4A antigen.) (1.3 MB)
CPTC-KRAS4B-4 IPMS
Result: Positive
This PDF shows the results of immuno-precipitation mass spectrometry (IPMS) assay of both KRAS4A and KRAS4B antigens using the CPTC-KRAS4B-4 antibody. (Note that this antibody does not pull down KRAS4A antigen.)
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (473.4 KB)
CPTC-KRAS4B-4 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Indirect ELISA
CPTC-KRAS4B-4 Indirect ELISA
Result: High Binding
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KRAS4B-4 Western blot
Result: Positive
Western blot of CPTC-KRAS4b-3 antibody with full length KRAS4b recombinant protein.
Characterization SOP Files
Background
NCI Identification Number:
00125
Antigen Name:
KRAS Proto-Oncogene, GTPase
CPTC Name:
CPTC-KRAS4B
Aliases:
Kirsten Rat Sarcoma Viral Oncogene Homolog; CFC2; KRAS1; KRAS Proto-Oncogene, GTPase; KRAS2; V-Ki-Ras2 Kirsten Rat Sarcoma 2 Viral Oncogene Homolog; GTPase KRas; C-Ki-Ras; K-Ras4B; K-Ras 2; RASK2; V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog; Kirsten Rat Sarcoma Viral Proto-Oncogene; Cellular Transforming Proto-Oncogene; Cellular C-Ki-Ras2 Proto-Oncogene; Transforming Protein P21; PR310 C-K-Ras Oncogene; C-Kirsten-Ras Protein; Proto-Oncogene GTPase; K-Ras P21 Protein; Oncogene KRAS2; EC 3.6.5.2; C-Ki-Ras2; ; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; C-K-Ras; KI-RAS; Ki-Ras; K-Ras; RALD; NS3; OES; NS
Function:
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal regionMutations in the RAS family of proteins are frequently observed across cancer types. The amino acid positions that account for the overwhelming majority of these mutations are G12, G13 and Q61. The different protein isoforms, despite their raw similarity, also behave very differently when expressed in non-native tissue types, likely due to differences in the C-terminal hyper-variable regions. Mis-regulation of isoform expression has been shown to be a driving event in cancer, as well as missense mutations at the three hotspots previously mentioned. While highly recurrent in cancer, attempts to target these RAS mutants with inhibitors have not been successful, and has not yet become common practice in the clinic. The prognostic implications for KRAS mutations vary between cancer types, but have been shown to be associated with poor outcome in colorectal cancer, non-small cell lung cancer, and others.KRAS (KRAS Proto-Oncogene, GTPase) is a Protein Coding gene. Diseases associated with KRAS include Oculoectodermal Syndrome and Schimmelpenning-Feuerstein-Mims Syndrome. Among its related pathways are Envelope proteins and their potential roles in EDMD physiopathology and MET promotes cell motility. Gene Ontology (GO) annotations related to this gene include GTP binding and protein-containing complex binding. An important paralog of this gene is HRAS.Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:20949621). Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).
Chromosomal Localization:
12p12.1
Expression System:
E. coli
Accession Number:
NP_004976.2
UniProt Accession Number:
P01116
DNA Source:
R999-X117-566
Immunogen:
Recombinant Full Length Protein
Vector Name:
pDest-566
Extinction Coefficient:
Buffers:
Expressed Sequence:
GGSGTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVID
GETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYR
EQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAK
TRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
21552
Last Updated:
07/06/2022
Links
- Entrez - Entrez KRAS 4B
- EnsEMBL - EnsEMBL KRAS 4B
- Uniprot - Uniprot P01116
Characterization Data
SOPs
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