PRINT

Kinetochore Scaffold 1 Peptide 1

Antibody: CPTC-KNL1-1
Background

Catalog Number:

CPTC-KNL1-1

Target Antigen:

Kinetochore Scaffold 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

04/19/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (288.5 KB)

CPTC-KNL1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue

CTPC-KNL1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-KNL1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5923 for non-phosphorylated peptide and https://assays.cancer.gov/CPTAC-5924 for phosphorylated peptide) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-KNL1-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-KNL1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5923 for non-phosphorylated peptide and https://assays.cancer.gov/CPTAC-5924 for phosphorylated peptide)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-KNL1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line. Click image to enlarge

CPTC-KNL1-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-KNL1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line.


Characterization SOP Files

[top]

Antigen: Kinetochore Scaffold 1 Peptide 1
Background

NCI Identification Number:

00297

Antigen Name:

Kinetochore Scaffold 1 Peptide 1

CPTC Name:

CPTC-KNL1 Peptide 1

Aliases:

Kinetochore Scaffold 1; Cancer/Testis Antigen 29; Cancer Susceptibility Candidate Gene 5 Protein; ALL1-Fused Gene From Chromosome 15q14 Protein; Protein Phosphatase 1, Regulatory Subunit 55; Microcephaly, Primary Autosomal Recessive 4; Blinkin, Bub-Linking Kinetochore Protein; Cancer Susceptibility Candidate 5; Kinetochore-Null Protein 1; CASC5; CT29; Kinetochore Null 1 Homolog (C. Elegans); Bub-Linking Kinetochore Protein; Kinetochore Null 1 Homolog; Protein D40/AF15q14; Protein CASC5; KIAA1570; PPP1R55; HSpc105; AF15q14; Blinkin; HKNL-1; MCPH4; Spc7; D40

Function:

The protein encoded by this gene is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. The encoded protein functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues. This gene was originally identified as a fusion partner with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14). Mutations in this gene cause autosomal recessive primary microcephaly-4 (MCPH4). Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their biological validity has not been confirmed. KNL1 (Kinetochore Scaffold 1) is a Protein Coding gene. Diseases associated with KNL1 include Microcephaly 4, Primary, Autosomal Recessive and Primary Microcephaly. Among its related pathways are Signaling by Rho GTPases and Mitotic Metaphase and Anaphase.Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.

Chromosomal Localization:

15q15.1

Accession Number:

NP_733468.3

UniProt Accession Number:

Q8NG31

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CSLS(ph)NPTPDYC(Cam)HDK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1540

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]