PRINT

Janus Kinase 1 Peptide 1

Antibody: CPTC-JAK1-1
Background

Catalog Number:

CPTC-JAK1-1

Target Antigen:

Janus Kinase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

External Links
Purchase
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-JAK1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6216) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-JAK1-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-JAK1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6216)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CPTC-JAK1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-JAK-1 as primary antibody against human Janus kinase 1 (a protein tyrosine kinase) (JAK1) recombinant protein (lane 2). Expected molecular weight - 133.1 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-JAK1-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-JAK-1 as primary antibody against human Janus kinase 1 (a protein tyrosine kinase) (JAK1) recombinant protein (lane 2). Expected molecular weight - 133.1 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-JAK1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 133.3 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-JAK1-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-JAK1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 133.3 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-JAK1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 133.3 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-JAK1-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-JAK1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 133.3 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

[top]

Antigen: Janus Kinase 1 Peptide 1
Background

NCI Identification Number:

00376

Antigen Name:

Janus Kinase 1 Peptide 1

CPTC Name:

CPTC-JAK1 Peptide 1

Aliases:

Janus Kinase 1; JAK1A; Tyrosine-Protein Kinase JAK1; EC 2.7.10.2; JAK1B; JTK3; EC 2.7.10; JAK-1; JAK1

Function:

This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants.AK1 (Janus Kinase 1) is a Protein Coding gene. Diseases associated with JAK1 include Breast Implant-Associated Anaplastic Large Cell Lymphoma and Polycythemia Vera. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and RET signaling. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is TYK2.Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:7615558). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529) as well as interleukin (IL)-10 receptor (PubMed:12133952).

Chromosomal Localization:

1p31.3

Accession Number:

NP_001307852.1

UniProt Accession Number:

P23458

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LSDPGIPITVLSR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]