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Interleukin 6 Receptor Peptide 1

Antibody: CPTC-IL6R-1
Background

Catalog Number:

CPTC-IL6R-1

Target Antigen:

Interleukin 6 Receptor Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (384.1 KB)

CPTC-IL6R-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-IL6R-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-IL6R-1 Western Blot Recombinant Protein

Result: Negative

Western blot using CPTC-IL6R-1 as primary antibody against human interleukin 6 receptor (IL6R), transcript variant 2, recombinant protein (lane 2). Expected molecular weight - 38.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-IL6R-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 51.5 kDa and 40.2 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-IL6R-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-IL6R-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 51.5 kDa and 40.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-IL6R-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 51.5 kDa and 40.2 kDa. Molecular weight standards are also included (lane 1). Jurkat are positive. All other cell lines are negative. Click image to enlarge

CPTC-IL6R-1 Western Blot Cell Lysate

Result: Positive

Western blot using CPTC-IL6R-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 51.5 kDa and 40.2 kDa. Molecular weight standards are also included (lane 1). Jurkat are positive. All other cell lines are negative.


Characterization SOP Files

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Antigen: Interleukin 6 Receptor Peptide 1
Background

NCI Identification Number:

00434

Antigen Name:

Interleukin 6 Receptor Peptide 1

CPTC Name:

CPTC-IL6R Peptide 1

Aliases:

Interleukin 6 Receptor; Interleukin-6 Receptor Subunit Alpha; IL-6 Receptor Subunit Alpha; Membrane Glycoprotein 80; CD126 Antigen; IL-6RA; CD126; Gp80; IL-6R Subunit Alpha; IL-6R-Alpha; IL-6R-1; IL-6R 1; IL6RA; IL6RQ; IL6Q; IL6R

Function:

This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.IL6R (Interleukin 6 Receptor) is a Protein Coding gene. Diseases associated with IL6R include Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus and Interleukin 6, Serum Level Of, Quantitative Trait Locus. Among its related pathways are Signaling by GPCR and RAF-independent MAPK1/3 activation. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and interleukin-6 receptor binding. An important paralog of this gene is CSF3R.Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal (PubMed:28265003). Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.

Chromosomal Localization:

1q21.3

Accession Number:

NP_000556.1

UniProt Accession Number:

P08887

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SPLSNVVCEWGPR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

09/28/2020

Links

Characterization Data

SOPs

No SOPs available.

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