Background
Catalog Number:
CPTC-IL1A-1
Target Antigen:
Interleukin 1 Alpha Peptide 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
02/15/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-IL1A-1
Characterization Data [Compare Characterization Data]
IHC Tissue
CPTC-IL1A-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
NCI 60 Protein Array
CPTC-IL1A-1 NCI Protein Array
Result: Positive
Protein Array in which CPTC-IL1A-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-IL1A-1 Western Blot Recombinant Protein
Result: Positive
Western blot using CPTC-IL1A-1 as primary antibody against human interleukin 1, alpha (IL1A) recombinant protein (lane 2) domain (Ser 113 - Ala 271). Expected molecular weight - 18.0 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IL1A-1 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-IL1A-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 30.6 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IL1A-1 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-IL1A-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 30.6 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IL1A-1 Western Blot (Tissue Lysate)
Result: Negative
Western blot using CPTC-IL1A-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 30.6 kDa. Vinculin was used as a loading control.
Characterization SOP Files
Background
Catalog Number:
CPTC-IL1A-2
Target Antigen:
Interleukin 1 Alpha Peptide 1
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
08/10/2022
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-IL1A-3
Target Antigen:
Interleukin 1 Alpha Peptide 1
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
08/10/2022
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
Background
NCI Identification Number:
00477
Antigen Name:
Interleukin 1 Alpha Peptide 1
CPTC Name:
CPTC-IL1A Peptide 1
Aliases:
Interleukin 1 Alpha; Hematopoietin-1; IL1F1; Pro-Interleukin-1-Alpha; Preinterleukin 1 Alpha; Interleukin-1 Alpha; IL-1 Alpha; IL1-ALPHA; IL-1A; IL1A; IL1
Function:
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. IL1A (Interleukin 1 Alpha) is a Protein Coding gene. Diseases associated with IL1A include Arthritis and Cholesteatoma Of Middle Ear. Among its related pathways are NF-KappaB Family Pathway and Diseases associated with the TLR signaling cascade. Gene Ontology (GO) annotations related to this gene include cytokine activity and interleukin-1 receptor binding.Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes.
Chromosomal Localization:
2q14.1
Accession Number:
NP_000566.3
UniProt Accession Number:
P01583
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
ANDQYLTAAALHNLDEAVK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2090
Last Updated:
12/17/2020
Links
- EnsEMBL - EnsEMBL IL1A
- Entrez - Entrez IL1A
- Uniprot - Uniprot P01583
Characterization Data
SOPs
No SOPs available.
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