Background
Catalog Number:
CPTC-IL18-1
RRID:
AB_1553714
Target Antigen:
Interleukin 18
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/23/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-IL18-1
External Links
- Human Protein Atlas CPTC-IL18-1
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC IL-18-1 HPA Evaluation
Result: Negative
This PDF describes the results of the evaluation of this antibody by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IHC NCI60
CPTC IL-18-1 IHC NCI60
Result: Positive
Immunohistochemistry of CPTC IL-18-1 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer:1:1000
Characterization SOP Files
IHC Tissue
CPTC IL-18-1 IHC Tissue
Result: Positive
Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC IL-18-1. Titer: 1:1000
Characterization SOP Files
Indirect ELISA
CPTC-IL18-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC IL-18-1 Protein Array
Result: Positive
Protein Array in which CPTC IL-18-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-IL18-1 Western Blot
Result: Positive
Western Blot using CPTC-IL18-1 as primary Ab against Ag 10190 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-IL18-2
RRID:
AB_1553716
Target Antigen:
Interleukin 18
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
03/09/2023
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-IL18-2
Publications
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (568.5 KB)
CPTC-IL18-2 evaluation by the Human Protein Atlas
Result: Negative
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IHC NCI60
CPTC-IL18-2 IHC NCI60
Result: Positive
Immunohistochemistry of CPTC-IL18-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:50
Characterization SOP Files
Indirect ELISA
CPTC-IL18-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-IL18-2 Protein Array
Result: Positive
Protein Array in which CPTC-IL18-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-IL18-2 Western Blot
Result: Positive
Western Blot using CPTC-IL18-2 as primary Ab against Ag 10190 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-IL18-3
RRID:
AB_2123661
Target Antigen:
Interleukin 18
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-IL18-3
Characterization Data [Compare Characterization Data]
CyTOF
CPTC-IL18-3 Immuno-Mass Cytometry
Result: Positive
Imaging mass cytometry on breast cancer tissue core using CPTC-IL18-3 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, pancreas, breast, lung, testis, endometrium, and appendix) and cancer tissues (breast, ovarian, lung, and prostate).
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (95.9 KB)
CPTC-IL18-3
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-IL18-3 IHC NCI60
Result: Positive
Immuno-histochemistry of CPTC-IL18-3 for NCI60 Cell Line Array at titer 1:3000
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Characterization SOP Files
IHC Tissue
CPTC-IL18-3 IHC Tissue
Result: Positive
Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-IL18-3.
Characterization SOP Files
Indirect ELISA
CPTC-IL18-3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-IL18-3 NCI 60 Protein Array
Result: Positive
Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-IL18-3 Western Blot
Result: Positive
Western Blot using CPTC-IL18-3 as primary Ab against Ag 10190 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10190
Antigen Name:
Interleukin 18
CPTC Name:
CPTC-IL18
Aliases:
IL-18; IG1F; IL-1g; IL1F4; MGC12320; Interferon gamma inducing factor
Function:
Interleukin-18 (IL-18) is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works together with IL-12 to induce cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon gamma or type II interferon that plays an important role in activating the macrophages or other cells. Apart from its physiological role, IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders. The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Chromosomal Localization:
11q22.2 - q22.3
Expression System:
E. Coli
Accession Number:
NM_001562
UniProt Accession Number:
Q14116
DNA Source:
HIP:HsCD00000423
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
7575
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSV
IRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMA
VTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDN
KMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
Native Sequence:
MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRN
LNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTI
SVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQ
FESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
Calculated Isoelectric Point:
4.54
Molecular Weight:
22598
Last Updated:
09/01/2008
Links
- EnsEMBL - EnsEMBL IL18
- Uniprot - Uniprot Q14116
Characterization Data
Gel
Click image to enlarge
Ag 10190
PAGE of Ag 10190 with molecular weight standards
Characterization SOP Files
SOPs
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