Background
Catalog Number:
CPTC-IFNGR1-1
Target Antigen:
Interferon Gamma Receptor 1 Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/10/2022
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-IFNGR1-2
Target Antigen:
Interferon Gamma Receptor 1 Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/10/2022
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-IFNGR1-3
Target Antigen:
Interferon Gamma Receptor 1 Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
07/03/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
External Links
- CPTAC Assay Portal CPTC-IFNGR1-3
Purchase
- DSHB CPTC-IFNGR1-3
Characterization Data [Compare Characterization Data]
IHC Tissue
CPTC-IFNGR1-3 IHC Tissue
Result: Positive
Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and membranous staining using Antibody CPTC-IFNGR1-3. Titer: 1:1000
Characterization SOP Files
Immuno-MRM
CPTC-IFNGR1-3 iMRM (Frozen Tissue)
Result: Positive
Immuno-MRM chromatogram of CPTC-IFNGR1-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6243)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue
Characterization SOP Files
NCI 60 Protein Array
CPTC-IFNGR1-3 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-IFNGR1-3 Western Blot Recombinant Protein
Result: Presumed Positive (with additional bands)
Western blot using CPTC-IFNGR1-3 as primary antibody against human interferon gamma receptor 1 (IFNGR1) recombinant protein (lane 2). Expected molecular weight - 52.5 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IFNGR1-3 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-IFNGR1-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 54.4 kDa and 21.5 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IFNGR1-3 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-IFNGR1-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 54.4 kDa and 21.5 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IFNGR1-3 Western Blot (Tissue Lysate)
Result: Negative
Western blot using CPTC-IFNGR1-3 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 54.4 kDa and 21.5 kDa. Vinculin was used as a loading control.
Characterization SOP Files
Background
NCI Identification Number:
00476
Antigen Name:
Interferon Gamma Receptor 1 Peptide 1
CPTC Name:
CPTC-IFNGR1 Peptide 1
Aliases:
Interferon Gamma Receptor 1; IFN-Gamma Receptor 1; IFN-Gamma-R-Alpha; CD119 Antigen; IFN-Gamma-R1; CDw119; CD119; Interferon-Gamma Receptor Alpha Chain; Interferon Gamma Receptor Alpha-Chain; Immune Interferon Receptor 1; Antiviral Protein, Type 2; AVP, Type 2; IMD27A; IMD27B; IFNGR1; IFNGR
Function:
This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.IFNGR1 (Interferon Gamma Receptor 1) is a Protein Coding gene. Diseases associated with IFNGR1 include Immunodeficiency 27A and Immunodeficiency 27B. Among its related pathways are all-trans-Retinoic Acid Mediated Apoptosis and Tuberculosis. Gene Ontology (GO) annotations related to this gene include cytokine receptor activity and interferon-gamma receptor activity. An important paralog of this gene is IL20RA.Associates with IFNGR2 to form a receptor for the cytokine interferon gamma (IFNG) (PubMed:7615558, PubMed:2971451, PubMed:7617032, PubMed:10986460). Ligand binding stimulates activation of the JAK/STAT signaling pathway (PubMed:7673114). Plays an essential role in the IFN-gamma pathway that is required for the cellular response to infectious agents.
Chromosomal Localization:
6q23.3
Accession Number:
NP_000407.1
UniProt Accession Number:
P15260
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
YVSLITSYQPFSLEK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1650
Last Updated:
12/17/2020
Links
- EnsEMBL - EnsEMBL IFNGR1
- Entrez - Entrez IFNGR1
- Uniprot - Uniprot P15260
Characterization Data
SOPs
No SOPs available.
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