Background
Catalog Number:
CPTC-IFIT3-1
Target Antigen:
Interferon Induced Protein With Tetratricopeptide Repeats 3 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/06/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length
Purchase
- DSHB CPTC-IFIT3-1
Characterization Data [Compare Characterization Data]
IHC Tissue
CPTC-IFIT3-1 IHC Tissue
Result: Presumed Positive
Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-IFIT3-1. Titer: 1:250
The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity.
Characterization SOP Files
NCI 60 Protein Array
CPTC-IFIT3-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-IFIT3-1 Western Blot Recombinant Protein
Result: Positive
Western blot using CPTC-IFIT3-1 as primary antibody against human interferon-induced protein with tetratricopeptide repeats 3
(IFIT3), transcript variant 2, recombinant protein (lane 2). Expected molecular weight - 55.8 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IFIT3-1 Automated Western Blot Cell Lysate
Result: Presumed Positive (with additional bands)
Automated western blot using CPTC-IFIT3-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 56.0 kDa. Molecular weight standards are also included (lane 1). Jurkat, A549, and NCI-H226 are presumed positive. All other cell lines are negative.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IFIT3-1 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-IFIT3-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 56.0 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IFIT3-1 Western Blot (Tissue Lysate)
Result: Negative
Western blot using CPTC-IFIT3-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 56.0 kDa. Vinculin was used as a loading control.
Characterization SOP Files
Background
NCI Identification Number:
00448
Antigen Name:
Interferon Induced Protein With Tetratricopeptide Repeats 3 Peptide 1
CPTC Name:
CPTC-IFIT3 Peptide 1
Aliases:
Interferon Induced Protein With Tetratricopeptide Repeats 3; Interferon-Induced Protein With Tetratricopeptide Repeats 4; Interferon-Induced Protein With Tetratricopeptide Repeats 3; CIG-49; IFI60; ISG60; RIG-G; Retinoic Acid-Induced Gene G Protein; Interferon-Induced 60 KDa Protein; GARG-49; IFI-60K; IFIT-3; IFIT-4; ISG-60; IFIT4; CIG49; IRG2; P60; Cig41; IFIT3
Function:
IFIT3 (Interferon Induced Protein With Tetratricopeptide Repeats 3) is a Protein Coding gene. Diseases associated with IFIT3 include Lupus Erythematosus and Systemic Lupus Erythematosus. Among its related pathways are Interferon gamma signaling and Innate Immune System. Gene Ontology (GO) annotations related to this gene include identical protein binding. An important paralog of this gene is IFIT2.IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.
Chromosomal Localization:
10q23.31
Accession Number:
NP_001026853.1
UniProt Accession Number:
O14879
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
QAEELIQQEHADQAEIR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1870
Last Updated:
09/30/2020
Links
- EnsEMBL - EnsEMBL IFIT3
- Entrez - Entrez IFIT3
- Uniprot - Uniprot O14879
Characterization Data
SOPs
No SOPs available.
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