Interferon Induced Protein With Tetratricopeptide Repeats 3 Peptide 1


Background

Catalog Number:

CPTC-IFIT3-1

Target Antigen:

Interferon Induced Protein With Tetratricopeptide Repeats 3 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

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Characterization Data [Compare Characterization Data]
  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer  showing cytoplasmic and membranous staining using Antibody CPTC-IFIT3-1. Titer: 1:250
The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity. Click image to enlarge

CPTC-IFIT3-1 IHC Tissue

Result: Presumed Positive

Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-IFIT3-1. Titer: 1:250
The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity.


  NCI 60 Protein Array

CPTC-IFIT3-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-IFIT3-1 as primary antibody against human interferon-induced protein with tetratricopeptide repeats 3
(IFIT3), transcript variant 2, recombinant protein (lane 2).  Expected molecular weight - 55.8 kDa.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-IFIT3-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-IFIT3-1 as primary antibody against human interferon-induced protein with tetratricopeptide repeats 3
(IFIT3), transcript variant 2, recombinant protein (lane 2). Expected molecular weight - 55.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-IFIT3-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates.  Expected molecular weight - 56.0 kDa.  Molecular weight standards are also included (lane 1).  Jurkat, A549, and NCI-H226 are presumed positive. All other cell lines are negative. Click image to enlarge

CPTC-IFIT3-1 Automated Western Blot Cell Lysate

Result: Presumed Positive (with additional bands)

Automated western blot using CPTC-IFIT3-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 56.0 kDa. Molecular weight standards are also included (lane 1). Jurkat, A549, and NCI-H226 are presumed positive. All other cell lines are negative.


  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-IFIT3-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates.  Expected molecular weight - 56.0 kDa.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-IFIT3-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-IFIT3-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 56.0 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-IFIT3-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 56.0 kDa. Vinculin was used as a loading control. Click image to enlarge

CPTC-IFIT3-1 Western Blot (Tissue Lysate)

Result: Negative

Western blot using CPTC-IFIT3-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 56.0 kDa. Vinculin was used as a loading control.


Characterization SOP Files

Background

NCI Identification Number:

00448

Antigen Name:

Interferon Induced Protein With Tetratricopeptide Repeats 3 Peptide 1

CPTC Name:

CPTC-IFIT3 Peptide 1

Aliases:

Interferon Induced Protein With Tetratricopeptide Repeats 3; Interferon-Induced Protein With Tetratricopeptide Repeats 4; Interferon-Induced Protein With Tetratricopeptide Repeats 3; CIG-49; IFI60; ISG60; RIG-G; Retinoic Acid-Induced Gene G Protein; Interferon-Induced 60 KDa Protein; GARG-49; IFI-60K; IFIT-3; IFIT-4; ISG-60; IFIT4; CIG49; IRG2; P60; Cig41; IFIT3

Function:

IFIT3 (Interferon Induced Protein With Tetratricopeptide Repeats 3) is a Protein Coding gene. Diseases associated with IFIT3 include Lupus Erythematosus and Systemic Lupus Erythematosus. Among its related pathways are Interferon gamma signaling and Innate Immune System. Gene Ontology (GO) annotations related to this gene include identical protein binding. An important paralog of this gene is IFIT2.IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.

Chromosomal Localization:

10q23.31

Accession Number:

NP_001026853.1

UniProt Accession Number:

O14879

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

QAEELIQQEHADQAEIR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1870

Last Updated:

09/30/2020

Links

Characterization Data

SOPs

No SOPs available.

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