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Interferon Induced Protein With Tetratricopeptide Repeats 2 Peptide 1

Antibody: CPTC-IFIT2-1
Background

Catalog Number:

CPTC-IFIT2-1

Target Antigen:

Interferon Induced Protein With Tetratricopeptide Repeats 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (478.9 KB)

CPTC-IFIT2-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of prostate cancer showing cytoplasmic and membranous staining using Antibody CPTC-IFIT2-1. Titer: 1:1000 Click image to enlarge

CPTC-IFIT2-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of prostate cancer showing cytoplasmic and membranous staining using Antibody CPTC-IFIT2-1. Titer: 1:1000


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (478.9 KB)

CPTC-IFIT2-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-IFIT2-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-IFIT2-1 as primary antibody against human interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) recombinant protein (lane 2). Expected molecular weight - 54.5 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-IFIT2-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-IFIT2-1 as primary antibody against human interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) recombinant protein (lane 2). Expected molecular weight - 54.5 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-IFIT2-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 54.6 kDa. Molecular weight standards are also included (lane 1). HeLa, Jurkat, and NCI-H226 cell lines are positive. All other cell lines are negative. Click image to enlarge

CPTC-IFIT2-1 Automated Western Blot Cell Lysate

Result: Positive

Automated western blot using CPTC-IFIT2-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 54.6 kDa. Molecular weight standards are also included (lane 1). HeLa, Jurkat, and NCI-H226 cell lines are positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-IFIT2-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 54.6 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-IFIT2-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-IFIT2-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 54.6 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Interferon Induced Protein With Tetratricopeptide Repeats 2 Peptide 1
Background

NCI Identification Number:

00437

Antigen Name:

Interferon Induced Protein With Tetratricopeptide Repeats 2 Peptide 1

CPTC Name:

CPTC-IFIT2 Peptide 1

Aliases:

Interferon Induced Protein With Tetratricopeptide Repeats 2; Interferon-Induced Protein With Tetratricopeptide Repeats 2; Interferon-Induced 54 KDa Protein; ISG-54 K; GARG-39; IFI-54K; ISG-54K; IFI-54; IFIT-2; G10P2; IFI54; ISG54; Cig42; P54; Interferon, Alpha-Inducible Protein (MW 54kD); Interferon-Induced Protein 54; CIG-42; IFIT2

Function:

IFIT2 (Interferon Induced Protein With Tetratricopeptide Repeats 2) is a Protein Coding gene. Diseases associated with IFIT2 include Microphthalmia With Limb Anomalies. Among its related pathways are Interferon gamma signaling and Innate Immune System. An important paralog of this gene is IFIT3.IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.

Chromosomal Localization:

10q23.31

Accession Number:

NP_001538.4

UniProt Accession Number:

P09913

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

AIHHFIEGVK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

09/29/2020

Links

Characterization Data

SOPs

No SOPs available.

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