Background
Catalog Number:
CPTC-IFIT1-1
Target Antigen:
Interferon Induced Protein With Tetratricopeptide Repeats 1 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/06/2024
Antigen Recognition(s):
Peptide, Endogenous
Purchase
- DSHB CPTC-IFIT1-1
External Links
- Human Protein Atlas CPTC-IFIT1-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (542.6 KB)
CPTC-IFIT1-1 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (542.6 KB)
CPTC-IFIT1-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
NCI 60 Protein Array
CPTC-IFIT1-1 NCI 60 Protein Array
Result: Positive
Protein Array in which CPTC-IFIT1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-IFIT1-1 Western Blot Recombinant Protein
Result: Positive
Western blot using CPTC-IFIT1-1 as primary antibody against human interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), transcript variant 2, recombinant protein (lane 2). Expected molecular weight - 55.2 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IFIT-1 Automated Western Blot Cell Lysate
Result: Presumed Positive (with additional bands)
Automated western blot using CPTC-IFIT-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 55.4 kDa and 51.7 kDa. Molecular weight standards are also included (lane 1). All cell lines are presumed positive except for PBMC.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IFIT1-1 Western Blot Cell Lysate
Result: Presumed Positive (with additional bands)
Western blot using CPTC-IFIT1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 55.4 kDa and 51.7 kDa. Molecular weight standards are also included (lane 1). HeLa is presumed positive. All other cell lines are negative.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IFIT1-1 Western Blot (Tissue Lysate)
Result: Negative
Western blot using CPTC-IFIT1-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 55.4 kDa and 51.7 kDa. Vinculin was used as a loading control.
Characterization SOP Files
Background
NCI Identification Number:
00447
Antigen Name:
Interferon Induced Protein With Tetratricopeptide Repeats 1 Peptide 1
CPTC Name:
CPTC-IFIT1 Peptide 1
Aliases:
Interferon Induced Protein With Tetratricopeptide Repeats 1; Interferon-Induced Protein With Tetratricopeptide Repeats 1; Interferon-Induced 56 KDa Protein; IFI-56K; IFIT-1; IFNAI1; G10P1; IFI56; ISG56; P56; Interferon, Alpha-Inducible Protein (MW 56kD); Interferon-Inducible MRNA 561; GARG-16; IFI-56; RNM561; IFIT1; C56
Function:
This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed.IFIT1 (Interferon Induced Protein With Tetratricopeptide Repeats 1) is a Protein Coding gene. Diseases associated with IFIT1 include Hepatitis C and Hepatitis. Among its related pathways are Interferon gamma signaling and Innate Immune System. Gene Ontology (GO) annotations related to this gene include RNA binding. An important paralog of this gene is IFIT1B.Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.
Chromosomal Localization:
10q23.31
Accession Number:
NP_001539.3
UniProt Accession Number:
P09914
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
VLDQIEFLDTK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1210
Last Updated:
09/30/2020
Links
- EnsEMBL - EnsEMBL IFIT1
- Entrez - Entrez IFIT1
- Uniprot - Uniprot P09914
Characterization Data
SOPs
No SOPs available.
Don't have Adobe Reader™?
Get it for free at Adobe.com