PRINT

Indoleamine 2,3-Dioxygenase 1 Peptide 1

Antibody: CPTC-IDO1-3
Background

Catalog Number:

CPTC-IDO1-3

Target Antigen:

Indoleamine 2,3-Dioxygenase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image The affinity and binding kinetics of CPTC-IDO1-3 and human indoleamine 2,3-dioxygenase 1 (IDO1) recombinant protein were measured using surface plasmon resonance. Buffer exchanged IDO1 recombinant protein was immobilized onto a Series S CM5 sensor chip. CPTC-IDO1-3 antibody at 1024 nM, 256 nM, 64 nM, 16 nM and 4 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a 1:1 fitting model. Click image to enlarge

CPTC-IDO1-3 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Positive

The affinity and binding kinetics of CPTC-IDO1-3 and human indoleamine 2,3-dioxygenase 1 (IDO1) recombinant protein were measured using surface plasmon resonance. Buffer exchanged IDO1 recombinant protein was immobilized onto a Series S CM5 sensor chip. CPTC-IDO1-3 antibody at 1024 nM, 256 nM, 64 nM, 16 nM and 4 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a 1:1 fitting model.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (330.8 KB)

CPTC-IDO1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence

CPTC-IDO1-3 Immunofluorescence

Result: Negative

Immunofluorescence staining of HeLa cells using CPTC-IDO1-3 antibody. IDO1 protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (330.8 KB)

CPTC-IDO1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  NCI 60 Protein Array

CTPC-IDO1-3 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500 Click image to enlarge

CPTC-IDO1-3 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-IDO1-3 as primary antibody against human indoleamine 2,3-dioxygenase 1 (IDO1) recombinant protein (lane 2). Expected molecular weight - 47.7 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-IDO1-3 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-IDO1-3 as primary antibody against human indoleamine 2,3-dioxygenase 1 (IDO1) recombinant protein (lane 2). Expected molecular weight - 47.7 kDa.
Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-IDO1-3 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 48 kDa. Molecular weight standards are also included (lane 1). Data is positive for buffy coat, presumed positive for A549 and NCI-H226. Data is negative/inconclusive for remaining cell lines. Click image to enlarge

CPTC-IDO1-3 Automated Western Blot (Cell Lysate)

Result: Presumed Positive (with additional bands)

Automated western blot using CPTC-IDO1-3 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 48 kDa. Molecular weight standards are also included (lane 1). Data is positive for buffy coat, presumed positive for A549 and NCI-H226. Data is negative/inconclusive for remaining cell lines.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-IDO1-3 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Molecular weight standards are also included (lane 1). Expected molecular weight - 45.3 kDa. A549 is presumed positive. All the other cell lines are negative. Click image to enlarge

CPTC-IDO1-3 Western Blot Cell Lysate

Result: Presumed Positive (with additional bands)

Western blot using CPTC-IDO1-3 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Molecular weight standards are also included (lane 1). Expected molecular weight - 45.3 kDa. A549 is presumed positive. All the other cell lines are negative.


Characterization SOP Files

[top]

Antigen: Indoleamine 2,3-Dioxygenase 1 Peptide 1
Background

NCI Identification Number:

00373

Antigen Name:

Indoleamine 2,3-Dioxygenase 1 Peptide 1

CPTC Name:

CPTC-IDO1 Peptide 1

Aliases:

Indoleamine 2,3-Dioxygenase 1; Indoleamine-Pyrrole 2,3-Dioxygenase; IDO-1; INDO; IDO; Indoleamine-Pyrrole 2,3 Dioxygenase; Indolamine 2,3 Dioxygenase; Indole 2,3-Dioxygenase; EC 1.13.11.52; IDO1

Function:

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.IDO1 (Indoleamine 2,3-Dioxygenase 1) is a Protein Coding gene. Diseases associated with IDO1 include Listeriosis and Trypanosomiasis. Among its related pathways are Histidine, lysine, phenylalanine, tyrosine, proline and tryptophan catabolism and superpathway of tryptophan utilization. Gene Ontology (GO) annotations related to this gene include heme binding and tryptophan 2,3-dioxygenase activity.Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).

Chromosomal Localization:

8p11.21

Accession Number:

NP_002155.1

UniProt Accession Number:

P14902

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

HLPDLIESGQLR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]