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Indoleamine 2,3-Dioxygenase 1 Peptide 3

Antibody: CPTC-IDO1-5
Background

Catalog Number:

CPTC-IDO1-5

Target Antigen:

Indoleamine 2,3-Dioxygenase 1 Peptide 3

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

05/04/2023

Antigen Recognition(s):

Peptide, Endogenous

Characterization Data [Compare Characterization Data]
  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing membranous staining using Antibody CPTC-IDO1-5. Titer: 1:50 Click image to enlarge

CPTC-IDO1-5 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing membranous staining using Antibody CPTC-IDO1-5. Titer: 1:50


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM using CPTC-IDO1-5 as capture antibody against the synthetic peptide NFLCSLESNPSVR. Antibody CPTC-IDO1-5 captures the synthetic peptide. Click image to enlarge

CPTC-IDO1-5 iMRM (synthetic peptide)

Result: Positive

Immuno-MRM using CPTC-IDO1-5 as capture antibody against the synthetic peptide NFLCSLESNPSVR. Antibody CPTC-IDO1-5 captures the synthetic peptide.


Characterization SOP Files

  Immunofluorescence

CPTC-IDO1-5 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-IDO1-5 as primary antibody against MCF7 cells shows no localization of IDO1 protein.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-IDO1-5 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-IDO1-5 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-IDO1-5 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-IDO1-5 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-IDO1-5 as primary antibody against human indoleamine 2,3-dioxygenase 1 (IDO1) recombinant protein. The expected molecular weight is 45.1 kDa.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated western blot using CPTC-IDO1-5 as primary antibody against recombinant IDO1 protein. Protein molecular weight is about 45 KDa. The antibody cannot recognize recombinant IDO1. Click image to enlarge

CPTC-IDO1-5 Automated Western Blot (Recombinant Protein)

Result: Negative

Automated western blot using CPTC-IDO1-5 as primary antibody against recombinant IDO1 protein. Protein molecular weight is about 45 KDa. The antibody cannot recognize recombinant IDO1.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-IDO1-5 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). The expected molecular weight is 45.3 kDa. CCRF-CEM, Jurkat, and MCF7 are presumed positive. HeLa is positive. K-562 is negative. Click image to enlarge

CPTC-IDO1-5 Western Blot (Cell Lysates)

Result: Positive

Western blot using CPTC-IDO1-5 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). The expected molecular weight is 45.3 kDa. CCRF-CEM, Jurkat, and MCF7 are presumed positive. HeLa is positive. K-562 is negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-IDO1-5 as primary antibody against whole lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 45 KDa. The antibody recognizes IDO1 in the HeLa and MCF7 lysates, presumably recognizes the target in CCRF-CEM and Jurkat, but not in K-562 lysates. Loading controls were run with anti-GAPDH antibody. Click image to enlarge

CPTC-IDO1-5 Automated Western Blot (Cell Lysates)

Result: Positive

Automated western blot using CPTC-IDO1-5 as primary antibody against whole lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 45 KDa. The antibody recognizes IDO1 in the HeLa and MCF7 lysates, presumably recognizes the target in CCRF-CEM and Jurkat, but not in K-562 lysates. Loading controls were run with anti-GAPDH antibody.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-IDO1-5 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-IDO1-5 as a primary antibody against MCF7 cell lysate. IDO1 protein expression was not detected.


Characterization SOP Files

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Antigen: Indoleamine 2,3-Dioxygenase 1 Peptide 3
Background

NCI Identification Number:

00523

Antigen Name:

Indoleamine 2,3-Dioxygenase 1 Peptide 3

CPTC Name:

CPTC-IDO1 Peptide 3

Aliases:

Indoleamine 2,3-Dioxygenase 1; INDO; IDO; Indoleamine-Pyrrole 2,3-Dioxygenase; IDO-1; Indoleamine-Pyrrole 2,3 Dioxygenase; Indolamine 2,3 Dioxygenase; Indole 2,3-Dioxygenase; EC 1.13.11.52

Function:

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.IDO1 (Indoleamine 2,3-Dioxygenase 1) is a Protein Coding gene. Diseases associated with IDO1 include Listeriosis and Trypanosomiasis. Among its related pathways are Histidine, lysine, phenylalanine, tyrosine, proline and tryptophan catabolism and superpathway of tryptophan utilization. Gene Ontology (GO) annotations related to this gene include heme binding and tryptophan 2,3-dioxygenase activity.Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).

Chromosomal Localization:

8p11.21

Accession Number:

NP_002155.1

UniProt Accession Number:

P14902

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NFLCSLESNPSVR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

06/09/2022

Links

Characterization Data

SOPs

No SOPs available.

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