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Heat Shock 60kDa Protein 1 (Chaperonin)

Antibody: CPTC-HSPD1-1
Background

Catalog Number:

CPTC-HSPD1-1

RRID:

AB_2617272

Target Antigen:

Heat Shock 60kDa Protein 1 (Chaperonin)

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

07/28/2021

Antigen Recognition(s):

Recombinant Full-length

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External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (84.5 KB)

CPTC-HSPD1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-HSPD1-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-HSPD1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-HSPD1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-HSPD1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-HSPD1-1 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-HSPD1-1 Western blot

Result: Positive

Western Blot using CPTC-HSPD1-1 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-HSPD1-2
Background

Catalog Number:

CPTC-HSPD1-2

RRID:

AB_2617273

Target Antigen:

Heat Shock 60kDa Protein 1 (Chaperonin)

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-HSPD1-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-HSPD1-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-HSPD1-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-HSPD1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-HSPD1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-HSPD1-2 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-HSPD1-2 Western blot

Result: Positive

Western Blot using CPTC-HSPD1-2 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-HSPD1-3
Background

Catalog Number:

CPTC-HSPD1-3

RRID:

AB_2617274

Target Antigen:

Heat Shock 60kDa Protein 1 (Chaperonin)

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-HSPD1-3 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-HSPD1-3 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-HSPD1-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-HSPD1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-HSPD1-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-HSPD1-3 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-HSPD1-3 Western blot

Result: Positive

Western Blot using CPTC-HSPD1-3 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Heat Shock 60kDa Protein 1 (Chaperonin)
Background

NCI Identification Number:

10793

Antigen Name:

Heat Shock 60kDa Protein 1 (Chaperonin)

CPTC Name:

CPTC-HSPD1

Aliases:

Heat shock 60kDa protein 1 (chaperonin); HSP60; GROEL; Mitochondrial matrix protein P1; Chaperonin 60; HuCHA60; P60 lymphocyte protein; HSP-60; 60 kDa chaperonin; CPN60; SPG13; HLD4; heat shock 60kD protein 1; spastic paraplegia 13; 60 kDa heat shock protein, mitochondrial; short heat shock protein 60 Hsp60s1; HSP65; heat shock protein 65; Heat shock protein 60; Hsp60

Function:

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of
imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Chromosomal Localization:

2q33.1

Expression System:

E. Coli

Accession Number:

NP_002147

UniProt Accession Number:

P10809

DNA Source:

HsCD00042457

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG19

Extinction Coefficient:

15993

Buffers:

PBS without Ca++ and Mg++

Expressed Sequence:

SNAMLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLL
ADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKL
VQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLA
VDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKG
VITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLS
EKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQV
VAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGE
VIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKL
SDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRC
IPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQS
SSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVT
EIPKEEKDPGMGAMGGMGGGMGGGMF

Native Sequence:

MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADA
VAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQD
VANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDA
VIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVIT
VKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKK
ISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAV
KAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIV
TKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDG
VAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPA
LDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSE
VGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIP
KEEKDPGMGAMGGMGGGMGGGMF

Calculated Isoelectric Point:

5.7

Molecular Weight:

61327

Last Updated:

08/22/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of HSPD1 (rAg 10793) with molecular weight standards in lane 1
Click image to enlarge

CPTC-HSPD1 PAGE

PAGE of HSPD1 (rAg 10793) with molecular weight standards in lane 1

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SOPs

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