Heat shock 60kDa protein 1 (chaperonin)


Background

Catalog Number:

CPTC-HSPD1-1

Target Antigen:

Heat shock 60kDa protein 1 (chaperonin)

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

06/13/2017

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-HSPD1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

  Western Blot
Click to enlarge image Western Blot using CPTC-HSPD1-1 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-HSPD1-1 Western blot

Western Blot using CPTC-HSPD1-1 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  NCI 60 Protein Array

CPTC-HSPD1-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC Tissue

CPTC-HSPD1-1 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

  IHC NCI60

CPTC-HSPD1-1 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Background

Catalog Number:

CPTC-HSPD1-2

Target Antigen:

Heat shock 60kDa protein 1 (chaperonin)

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

04/29/2014

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-HSPD1-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

  Western Blot
Click to enlarge image Western Blot using CPTC-HSPD1-2 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-HSPD1-2 Western blot

Western Blot using CPTC-HSPD1-2 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  NCI 60 Protein Array

CPTC-HSPD1-2 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC Tissue

CPTC-HSPD1-2 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

  IHC NCI60

CPTC-HSPD1-2 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Background

Catalog Number:

CPTC-HSPD1-3

Target Antigen:

Heat shock 60kDa protein 1 (chaperonin)

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

03/06/2014

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-HSPD1-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

  Western Blot
Click to enlarge image Western Blot using CPTC-HSPD1-3 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-HSPD1-3 Western blot

Western Blot using CPTC-HSPD1-3 as primary Ab against HSPD1 (rAg 10793) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  NCI 60 Protein Array

CPTC-HSPD1-3 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC Tissue

CPTC-HSPD1-3 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

  IHC NCI60

CPTC-HSPD1-3 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Background

NCI Identification Number:

10793

Antigen Name:

Heat shock 60kDa protein 1 (chaperonin)

CPTC Name:

CPTC-HSPD1

Keywords:

Heat shock 60kDa protein 1 (chaperonin); HSP60; GROEL; Mitochondrial matrix protein P1; Chaperonin 60; HuCHA60; P60 lymphocyte protein; HSP-60; 60 kDa chaperonin; CPN60; SPG13; HLD4; heat shock 60kD protein 1; spastic paraplegia 13; 60 kDa heat shock protein, mitochondrial; short heat shock protein 60 Hsp60s1; HSP65; heat shock protein 65; Heat shock protein 60; Hsp60

Function:

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of
imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Chromosomal Localization:

2q33.1

Expression System:

E. Coli

NCBI Accession Number:

NP_002147

Swiss Prot Accession Number:

P10809

DNA Source:

HsCD00042457

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG19

Extinction Coefficient:

15993

Buffers:

PBS without Ca++ and Mg++

Expressed Sequence:

SNAMLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLL
ADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKL
VQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLA
VDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKG
VITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLS
EKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQV
VAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGE
VIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKL
SDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRC
IPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQS
SSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVT
EIPKEEKDPGMGAMGGMGGGMGGGMF

Native Sequence:

MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADA
VAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQD
VANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDA
VIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVIT
VKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKK
ISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAV
KAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIV
TKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDG
VAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPA
LDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSE
VGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIP
KEEKDPGMGAMGGMGGGMGGGMF

Calculated Isoelectric Point:

5.7

Molecular Weight:

61327

Last Updated:

07/18/2019

Links

Characterization Data

Gel

Click to enlarge image PAGE of HSPD1 (rAg 10793) with molecular weight standards in lane 1
Click image to enlarge

CPTC-HSPD1 PAGE

PAGE of HSPD1 (rAg 10793) with molecular weight standards in lane 1

SOPs

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