Background
Catalog Number:
CPTC-HAVCR2-1
Target Antigen:
Hepatitis A Virus Cellular Receptor 2 Peptide 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
Immuno-Oncology (IO)
External Links
- CPTAC Assay Portal CPTAC-5984
Publications
Characterization Data [Compare Characterization Data]
Immuno-MRM
CPTC-HAVCR2-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-HAVCR2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5984)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.
Characterization SOP Files
NCI 60 Protein Array
CPTC-HAVCR2-1
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-HAVCR2-1 Western Blot Recombinant Protein
Result: Negative
Western blot using CPTC-HAVCR2-1 as primary antibody against human T-cell immunoglobulin mucin receptor 3 (TIM3), recombinant protein (lane 2). Expected molecular weight - 23 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-HAVCR2-1 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-HAVCR2-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 33.4 kDa and 16.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-HAVCR2-1 Automated Western Blot Cell Lysate
Result: Presumed Positive (with additional bands)
Automated western blot using CPTC-HAVCR2-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 33.4 kDa and 16.1 kDa. Molecular weight standards are also included (lane 1). A549 and MCF7 are presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.
Characterization SOP Files
Background
Catalog Number:
CPTC-HAVCR2-2
Target Antigen:
Hepatitis A Virus Cellular Receptor 2 Peptide 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
10/18/2023
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-HAVCR2-3
Target Antigen:
Hepatitis A Virus Cellular Receptor 2 Peptide 1
Isotype:
IgG
Species:
Mouse Monoclonal Antibody
Last Updated:
10/28/2021
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
Background
NCI Identification Number:
00474
Antigen Name:
Hepatitis A Virus Cellular Receptor 2 Peptide 1
CPTC Name:
CPTC-HAVCR2 Peptide 1
Aliases:
Hepatitis A Virus Cellular Receptor 2; TIMD3; TIM3; T-Cell Immunoglobulin And Mucin Domain-Containing Protein 3; T-Cell Immunoglobulin Mucin Family Member 3; T-Cell Immunoglobulin Mucin Receptor 3; T-Cell Membrane Protein 3; HAVcr-2; TIMD-3; CD366; Tim-3; T Cell Immunoglobulin Mucin 3; Kidney Injury Molecule-3; CD366 Antigen; FLJ14428; HAVCR2; KIM-3; SPTCL; TIM-3
Function:
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. HAVCR2 (Hepatitis A Virus Cellular Receptor 2) is a Protein Coding gene. Diseases associated with HAVCR2 include T-Cell Lymphoma, Subcutaneous Panniculitis-Like and Panniculitis. Among its related pathways are RET signaling and Innate Immune System. An important paralog of this gene is HAVCR1.Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:24825777). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556005). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse (PubMed:24337741, PubMed:26492563). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (By similarity). Expressed on Treg cells can inhibit Th17 cell responses (PubMed:24838857). Receptor for LGALS9 (PubMed:16286920, PubMed:24337741). Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6. Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (By similarity). However, the function as receptor for LGALS9 has been challenged (PubMed:23555261). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (By similarity). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent. May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells. Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells. Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha. In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (By similarity). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9 (PubMed:22323453). In contrast, shown to suppress NK cell-mediated cytotoxicity (PubMed:22383801). Negatively regulates NK cell function in LPS-induced endotoxic shock (By similarity).
Chromosomal Localization:
5q33.3
Accession Number:
NP_116171.3
UniProt Accession Number:
Q8TDQ0
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
GACPVFECGNVVLR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1540
Last Updated:
12/17/2020
Links
- EnsEMBL - EnsEMBL HAVCR2
- Entrez - Entrez HAVCR2
- Uniprot - Uniprot Q8TDQ0
Characterization Data
SOPs
No SOPs available.
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